Chemical Characterization of Honeysuckle Polyphenols and Their Alleviating Function on Ultraviolet B-Damaged HaCaT Cells by Modulating the Nrf2/NF-κB Signaling Pathways

Antioxidants, Journal Year: 2024, Volume and Issue: 13(3), P. 294 - 294

Published: Feb. 28, 2024

Scientific evidence attests that the epidermis receives excessive ultraviolet B (UVB) radiation, triggering generation of substantial quantities reactive oxygen species (ROS), which disrupted delicate equilibrium oxidation–reduction, leading to oxidative stress and inflammation. The historical use honeysuckle polyphenols (HPs) has garnered our attention due their efficacy in inhibiting damage. In this study, HPs were prepared from flowers employing an ultrasonic-assisted extraction method quantitatively analyzed by a LC–MS/MS, mechanisms underlying HPs’ antioxidative anti-inflammatory effects on UVB-irradiated HaCaT cell model systematically investigated. results showed had significant cellular repair effect cells (p < 0.001). mechanism action indicated could allow Nrf2 enter nucleus regulating dissociation Keap1, further increases activity downstream proteases (SOD CAT), ROS scavenging, reduces intracellular malondialdehyde (MDA) level. addition, down-regulate Toll-like receptor 4 (TLR4) inhibit NF-κB (P65) dissociating IκBα, resulting decrease entry into inflammatory factors (TNF-α, IL-6, IL-1β). four key compounds HPs, including chlorogenic acid, quercetin, isorhamnetin, luteolin, selected verify repairing UVB damage using molecular docking techniques. experiment suggested active effectively occupy Kelch homologue (Kelch) structural domain competitively bind with Nrf2, facilitate promotion binding, ultimately enhancing translocation nucleus. interact through hydrogen bonding, van der Waals forces, electrostatic forces its summary, can radiation be used develop health cosmetic products for treatment UV radiation-induced diseases.

Language: Английский

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Medicinal Plant Extracts Targeting UV-Induced Skin Damage: Molecular Mechanisms and Therapeutic Potential

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2278 - 2278

Published: March 4, 2025

The depletion of the ozone layer has intensified ultraviolet (UV) radiation exposure, leading to oxidative stress, DNA damage, inflammation, photoaging, and skin cancer. Medicinal plants, widely used in Traditional Herbal Medicine (THM), particularly Chinese (TCM), have demonstrated significant therapeutic potential due their well-characterized active compounds established photoprotective effects. This review systematically evaluates 18 medicinal plants selected based on traditional use skin-related conditions emerging evidence supporting efficacy against UV-induced damage. Their bioactive components exert antioxidant, anti-inflammatory, repair, depigmentation effects by modulating key signaling pathways, including Nrf2/ARE-, MAPK/AP-1-, PI3K/Akt-, MITF/TYR-related melanogenesis pathways. Moreover, novel drug delivery systems, such as exosomes, hydrogels, nanoemulsions, significantly enhanced stability, bioavailability, penetration these compounds. However, challenges remain standardizing plant-derived formulations, elucidating complex synergistic mechanisms, translating preclinical findings into clinical applications. Future interdisciplinary research technological advancements will be essential harness full for damage prevention treatment.

Language: Английский

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Evaluating the impact of ecological factors on the quality and habitat distribution of Lonicera japonica Flos using HPLC and the MaxEnt model

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 6, 2024

The quality of traditional Chinese medicine is based on the content their secondary metabolites, which vary with habitat adaptation and ecological factors. This study focuses

Language: Английский

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Pharmacological Activities of Lonicerae japonicae flos and Its Derivative—“Chrysoeriol” in Skin Diseases

Molecules, Journal Year: 2024, Volume and Issue: 29(9), P. 1972 - 1972

Published: April 25, 2024

Chrysoeriol is an active ingredient derived from the Chinese medicinal herb (CMH) “Lonicerae japonicae flos” in dried flower bud or bloomed of Lonicera japonica Thunberg. Dermatoses are most common diseases humans, including eczema, acne, psoriasis, moles, and fungal infections, which temporary permanent may be painless painful. Topical corticosteroids widely used Western medicine, but there some side effects when it continuously regularly utilized a large dosage. natural ingredient, nontoxic, without any adverse reactions treatment dermatological conditions. Methods: Nine electronic databases were searched, WanFang Data, PubMed, Science Direct, Scopus, Web Science, Springer Link, SciFinder, China National Knowledge Infrastructure (CNKI), regard to language constraints. The pharmacological activities chrysoeriol Lonicerae flos fight against skin explained evaluated through literature review either vitro vivo studies. Results: decreased mRNA levels proinflammatory cytokines IL-6, IL-1β, TNF-α. These transcriptionally regulated by NF-κB STAT3 combat inflammation. It also showed promising actions treating many ailments wound healing, depigmentation, photoprotection, antiaging. Conclusion: cutaneous route best delivery approach across barrier. However, toxicity, dosage, safety assessments formulation nanochrysoeriol on human epidermis for application must further investigated.

Language: Английский

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Research progress on the biosynthesis, metabolic engineering, and pharmacology of bioactive compounds from the <i>Lonicera</i> genus

Medicinal Plant Biology, Journal Year: 2024, Volume and Issue: 3(1), P. 0 - 0

Published: Jan. 1, 2024

The Lonicera genus with medicinal and edible characteristics is a rich source of bioactive compounds that together account for numerous health benefits. Plants within this have been used traditionally in China to treat heat-related illnesses, viral respiratory infections, skin diseases, inflammation thousands years. main include chlorogenic acids, flavonoids, iridoids, triterpene saponins. Aside from the role as valuable resource, constituent members also food cosmetic industries. Due huge demand plants propensity overexploitation wild resources Lonicera, there need meet via alternative efficient strategies such metabolic engineering green production. Here, we provide comprehensive review relevant scientific literature covering structure, pharmacology, multi-omics analyses, phylogenetic analysis, biosynthesis, constituents genus. Finally, proffer suggestions on prospects fully exploiting utilizing useful plant resources.

Language: Английский

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A Low-Modulus Phosphatidylserine-Exposing Microvesicle Alleviates Skin Inflammation via Persistent Blockade of M1 Macrophage Polarization

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 394 - 394

Published: Jan. 4, 2025

Inflammatory skin diseases comprise a group of conditions characterized by damage to function due overactive immune responses. These disorders not only impair the barrier but also deteriorate quality life and increase risk psychiatric issues. Here, low-modulus phosphatidylserine-exposing microvesicle (deformed PSV, D-PSV) was produced, characterized, evaluated for its potential therapeutic against diseases. Compared conventional PSVs (C-PSVs), D-PSVs exhibited more robust longer-lasting inhibitory effect on inflammatory response triggered lipopolysaccharides interferon-γ in primary bone marrow-derived macrophage model. Transcriptome analysis indicated that mainly achieved modulating inflammation-related signaling pathways, leading reduction expressions pro-inflammatory genes. In an imiquimod-induced psoriatic dermatitis mouse model, topical application effectively mitigated inflammation microenvironment reduced lesion severity. improvements were attributed superior permeability persistent adhesion macrophages compared with C-PSVs. summary, this macrophage-targeted offers promising non-invasive approach managing persistently inhibiting M1 polarization restoring balance.

Language: Английский

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Genome-wide identification and expression profiling of the NCED gene family in salt and drought stress response of honeysuckle (Lonicera japonica thunb.)

Scientia Horticulturae, Journal Year: 2025, Volume and Issue: 341, P. 113980 - 113980

Published: Jan. 18, 2025

Language: Английский

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Activation of the phenylpropanoid pathway and enhanced powdery mildew resistance in honeysuckle induced by inactivated Erysiphe lonicerae spores

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102739 - 102739

Published: May 1, 2025

Language: Английский

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Potential Antioxidant and Anti-Inflammatory Properties of Polyphenolic Compounds from Cirsium japonicum Extract

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 785 - 785

Published: Jan. 8, 2024

Cirsium japonicum is a medicinal plant that has been used due to its beneficial properties. However, extensive information regarding therapeutic potential scarce in the scientific literature. The antioxidant and anti-inflammatory of polyphenols derived from extracts (CJE) was systematically analyzed. High-performance liquid chromatography (HPLC) with mass spectrometry (MS) examine compounds CJE. A total six peaks polyphenol were identified extract, their MS data also confirmed. These bioactive subjected ultrafiltration LC analysis assess for targeting cyclooxygenase-2 (COX2) DPPH. outcomes showed which primary had highest affinity binding both COX2 This suggests components excellent ability DPPH can be considered significant active substances. Additionally, vitro CJE carried out macrophage cells after inducing inflammation lipopolysaccharide (LPS). As result, it downregulated expression two critical pro-inflammatory cytokines, inducible nitric oxide synthase (iNOS). In addition, we found solid through molecular docking selected inflammatory mediators. conclusion, polyphenolic extract confirmed effects. results may provide application food pharmaceutical industries further analysis.

Language: Английский

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Binding affinity screening of polyphenolic compounds in Stachys affinis extract (SAE) for their potential antioxidant and anti-inflammatory effects

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 5, 2024

Free radical is a marker in various inflammatory diseases. The antioxidant effect protects us from this damage, which also plays an essential role preventing inflammation. Inflammation the body biological stimuli, and pro-inflammatory mediators are negatively affected immune system. caused by LPS endotoxin found outer membrane of Gram-negative bacteria, induces cells to produce cytokines such as cyclooxygenase-2 (COX-2) inducible nitric oxide synthase. Based on this, anti-inflammatory effects plant extracts were investigated. First, main phenolic compounds for five peaks obtained Stachys affinis extract (SAE) identified. each compound was confirmed through HPLC analysis before after competitive binding reaction between DPPH extract. Afterward, COX2 analysis. Lastly, SAE vitro experiments terms structural molecular docking. This study that have potential effects, may provide information primary screening medicinal plants.

Language: Английский

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