The hydrogen sulfide donor sodium thiosulfate limits inflammation but aggravate smooth muscle cells apoptosis and aneurysm progression in a mouse model of abdominal aortic aneurysm DOI Creative Commons

Clémence Bechelli,

Diane Macabrey,

Florian Caloz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 17, 2023

Abstract Intro The prevalence of abdominal aortic aneurysm (AAA) is constantly progressing with the aging global population. AAA rupture has a devastating 80% mortality rate and there no treatment to slow-down progression. Hydrogen sulfide (H 2 S) ubiquitous redox-modifying gasotransmitter produced in cardiovascular system via reverse trans-sulfuration pathway by cystathionine γ-lyase (CSE). H S protective properties on system, including anti-inflammatory antioxidant effects. Here, we hypothesized that sodium thiosulfate (STS), clinically relevant source S, would limit growth. Methods 8-12 weeks old male WT or Cse -/- mice C57BL/6J genetic background were submitted model topical elastase application aorta β-aminopropionitrile fumarate drinking water for post-op. Sodium (STS) was given post-op until collection. In vitro experiments conducted assess effect STS pro-inflammatory cytokines interleukin-1 β 6 tumor necrosis factor α primary human vascular smooth muscle cell (VSMC). Results Surprisingly, increased elastin degradation, size rupture, despite reducing infiltration macrophages, antigen-presenting cells lymphocytes mice. Conversely, impaired production developed smaller than immune cells. reduced VSMC coverage, possibly lowered proliferation, promoted loss extracellular matrix (ECM) breakdown. vitro, aggravated cytokine-induced VSMCs apoptosis. Conclusion paradoxical growth, inflammation while simultaneously impeding favorable remodeling, resulting bigger periadventitial elastase. This study identifies negative this environment, highlighting complex role deleterious progression significant, especially growing use clinical settings various indications.

Language: Английский

Effect of Rab8a on proliferation and migration of esophageal squamous cell carcinoma cells and its molecular mechanism DOI Creative Commons
Rong Liu,

Zhanting Kang,

Ting Gan

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 27, 2024

Abstract Purpose To explore the expression and function of Rab8a in esophageal squamous cell carcinoma (ESCC). Methods The study first used TIMER, GEPIA UALCAN to analyze a variety clinically common malignancies including ESCC, followed by real-time PCR (quantitative PCR, qPCR), Western blot, immunohistochemical (IHC) tests, series vitro biological experiments. Results is highly expressed cancer cells tissues, its significantly correlated with size depth invasion carcinoma. overexpression can promote proliferation migration ESCC while knockdown inhibit indicating positive correlation NDUFA1 CYC 1 through GEO database analysis. Therefore, may progression activating mitochondrial respiration. Conclusions This demonstrated that upregulated provides rationale for clinical diagnosis screening new therapeutic targets ESCC.

Language: Английский

Citations

0
The biological consequences of knockout of genes involved in the synthesis and metabolism of H2S inDrosophila melanogaster DOI Open Access
Victoria Shilova, Lyubov N. Chuvakova, D. G. Garbuz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

Abstract Here, we describe the effect of knockout (KO) two key genes ( cbs , cse ) responsible for H 2 S synthesis in transsulfuration pathway as well inactivation sulfurtransferase gene dtst1 encoding single domain rhodanese on Drosophila melanogaster genome expression and several life-history traits. The analysis production KO flies showed its minimal level strain with double CBS-/-; CSE-/- while triple CBS -/-; CSE dTST1 -/-) exhibited higher improvements lifespan. transcriptomic fly bodies from maximum disturbances fitness components revealed a profound increase involved functioning excretory system. Besides, drastic changes Malpighian tubules’ appearance. Inactivation related to metabolism resulted altered numerous loci mitochondrial function. While did not affect expression, more whole-body transcriptome than flies. Reduced fecundity knockouts correlates pronounced ovarian data. Surprisingly, affected flies’ memory.

Language: Английский

Citations

0
Indices of carbohydrate metabolism and antioxidant system state during germination of aged wheat and triticale seeds treated with H(2)S donor DOI Creative Commons
Т. О. Yastreb, А. И. Кокорев,

A. I. Dyachenko

et al.

The Ukrainian Biochemical Journal, Journal Year: 2024, Volume and Issue: 96(5), P. 79 - 95

Published: Oct. 23, 2024

Hydrogen sulfide is a gasotransmitter molecule involved in the realization of many functions plant organism, including seed germination. Aging seeds shown to be accompanied by oxidative stress and reduced The effect exogenous hydrogen on germination aged cereal has not been studied. aim work was estimate priming with NaHS as an H2S donor wheat triticale previously subjected natural aging. Seeds winter (Triticum aestivum) ×Triticosecale were stored indoors for 4 years at fluctuating temperature humidity. Aged treated 0.2-5 mM solution 3 h germinated Petri dishes days. hydropriming­ treatment used control. Amylase activity grains, biomass shoots roots, content total sugars, H2O2, lipid peroxidation products anthocyanin, antioxidant enzymes seedlings determined. It that after donor, activities catalase guaiacol peroxidase, well anthocyanins increased only seedlings. Nevertheless, both species followed enhanced growth increase amylase superoxide dismutase activities, decrease H2O2 MDA contents, elevated accumulation sugars shoots. concluded under influence caused mobilization reserve carbohydrates modulation system activity. Such can considered effective tool improve seedling growth. Keywords: amylase, system, sulfide, stress, aging, sugar, Triticosecale, Triticum aestivum

Language: Английский

Citations

0
Intoxications par l’hydrogène sulfuré DOI

F. Testud

EMC - Pathologie professionnelle et de l environnement, Journal Year: 2024, Volume and Issue: 43(3), P. 1 - 9

Published: Sept. 1, 2024

Citations

0
The hydrogen sulfide donor sodium thiosulfate limits inflammation but aggravate smooth muscle cells apoptosis and aneurysm progression in a mouse model of abdominal aortic aneurysm DOI Creative Commons

Clémence Bechelli,

Diane Macabrey,

Florian Caloz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 17, 2023

Abstract Intro The prevalence of abdominal aortic aneurysm (AAA) is constantly progressing with the aging global population. AAA rupture has a devastating 80% mortality rate and there no treatment to slow-down progression. Hydrogen sulfide (H 2 S) ubiquitous redox-modifying gasotransmitter produced in cardiovascular system via reverse trans-sulfuration pathway by cystathionine γ-lyase (CSE). H S protective properties on system, including anti-inflammatory antioxidant effects. Here, we hypothesized that sodium thiosulfate (STS), clinically relevant source S, would limit growth. Methods 8-12 weeks old male WT or Cse -/- mice C57BL/6J genetic background were submitted model topical elastase application aorta β-aminopropionitrile fumarate drinking water for post-op. Sodium (STS) was given post-op until collection. In vitro experiments conducted assess effect STS pro-inflammatory cytokines interleukin-1 β 6 tumor necrosis factor α primary human vascular smooth muscle cell (VSMC). Results Surprisingly, increased elastin degradation, size rupture, despite reducing infiltration macrophages, antigen-presenting cells lymphocytes mice. Conversely, impaired production developed smaller than immune cells. reduced VSMC coverage, possibly lowered proliferation, promoted loss extracellular matrix (ECM) breakdown. vitro, aggravated cytokine-induced VSMCs apoptosis. Conclusion paradoxical growth, inflammation while simultaneously impeding favorable remodeling, resulting bigger periadventitial elastase. This study identifies negative this environment, highlighting complex role deleterious progression significant, especially growing use clinical settings various indications.

Language: Английский

Citations

0