Specific Antimicrobial Activities Revealed by Comparative Evaluation of Selected Gemmotherapy Extracts

Antibiotics, Journal Year: 2024, Volume and Issue: 13(2), P. 181 - 181

Published: Feb. 13, 2024

Nowadays, unprecedented health challenges are urging novel solutions to address antimicrobial resistance as multidrug-resistant strains of bacteria, yeasts and moulds emerging. Such microorganisms can cause food feed spoilage, poisoning even more severe diseases, resulting in human death. In order overcome this phenomenon, it is essential identify antimicrobials that naturally occurring, biologically effective increasingly safe for use. The development gemmotherapy extracts (GTEs) using plant parts such buds young shoots has emerged a approach treat/prevent conditions due their associated antidiabetic, anti-inflammatory and/or properties all require careful evaluations. Seven GTEs obtained from species like the olive (Olea europaea L.), almond (Prunus amygdalus black mulberry (Morus nigra walnut (Juglans regia blackberry (Rubus fruticosus blackcurrant (Ribes nigrum L.) bilberry (Vaccinium myrtillus were tested efficiency via agar diffusion microbroth dilution methods. activity was assessed eight bacterial (Bacillus cereus, Staphylococcus aureus, Salmonella enterica subsp. enterica, Proteus vulgaris, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa Listeria monocytogenes), five (Aspergillus flavus, Aspergillus niger, ochraceus, Penicillium citrinum, expansum) one yeast strain (Saccharomyces cerevisiae). method revealed GTE most since inhibited growth three bacterial, four species, having considered total number affected microorganism species. Next blackberry, appeared be second efficient, suppressing but no or yeasts. minimum inhibitory concentration (MIC) bactericidal (MBC) then determined each tested. Noticeably, feature strongest bacteriostatic outcome, displaying specificity S. E. faecalis L. monocytogenes. other GTEs, blueberry, walnut, (the list indicates relative strength), at microbial than inducing However, some specificities also evident, while had significant activity. Having seen analysed especially these could envisioned potential might enhance antibiotic therapies efficiency, would act an antifungal agent. Some mixtures have shown interesting synergies, effects observed argue extending studies include pathological microorganisms.

Language: Английский

Hepatoprotective Activity and Oxidative Stress Reduction in an Arctium tomentosum Mill. Root Extract in Mice with Experimentally Induced Hepatotoxicity

Livers, Journal Year: 2024, Volume and Issue: 4(4), P. 696 - 710

Published: Dec. 17, 2024

Background: The use of natural hepatoprotective remedies represents an important path in modern phytotherapy. Objectives: In this context, our research aims to evaluate the phytochemical composition and oxidative stress reduction potential Arctium tomentosum Mill. root extract. Methods: phenolic profile tested extract, prepared by subcritical fluid-assisted method were qualitatively quantitatively analyzed spectrophotometrical HPLC/DAD/ESI methods. vitro antioxidant capacity was assessed using DPPH FRAP assays. Hepatoprotective activity extract on a model CCl4 experimentally induced hepatotoxicity mice. Results: Phytochemical assays revealed presence polyphenols, such as chlorogenic acid (17.20 ± 0.65 μg/mL) acacetin 7-O-glucoside (56.80 1.66 μg/mL). vitro, exhibited significant capacity, while vivo it showed dose-dependent effect indicated improvement plasma proteins down-regulation transaminase (ALAT, ASAT, GGT). liver tissue, partially restored GPx, CAT, SOD attenuated lipid peroxidation. protective A. supported alleviation histological injuries (centrilobular necrosis, granulocytic infiltrate, fibrosis). Conclusions: proved be able provide mainly through mechanism.

Language: Английский