Olfactory mucosal mesenchymal stem cell-derived exosome Lnc A2M-AS1 ameliorates oxidative stress by regulating TP53INP1-mediated mitochondrial autophagy through interacting with IGF2BP1 in Parkinson’s diseases DOI Creative Commons
Jiangshan Zhang, Chuang Wang, Guoshuai Yang

et al.

Cell Biology and Toxicology, Journal Year: 2025, Volume and Issue: 41(1)

Published: March 20, 2025

Abstract Background Exosome Lnc A2M-AS1 from olfactory mucosa mesenchymal stem cells (OM-MSCs) can ameliorate oxidative stress by improving mitophagy in cardiomuscular cells; however, it remains unclear whether this effect exists the brain tissues of patients with Parkinson’s disease (PD). Methods OM-MSC–Exosomes were isolated and verified based on morphology specific biomarkers. The effects OM-MSC-Exo mitochondrial autophagy, stress, lncRNA detected MPP + -treated HT22 cells. OM-MSC-Exos autophagy an MPTP-induced Parkinson's (PD) model C57BL/6 mice. interaction between IGF2BP1, A2M-AS1, TP53INP1 was assessed via RNA pull-down/RNA Immunoprecipitation stability assays. lnc IGF2BP1/TP53INP1-mediated PD mouse models. Results Exosomes found to be rich A2M-AS1. proved able induced regulates enhancing In addition, through mediated expression binding IGF2BP1. Furthermore, treatment improved symptoms ameliorated Conclusion Collectively, OM-MSC-derived exosomes targeting IGF2BP1 induce stress. could potentially serve as promising candidates for new methods PD. Graphical

Language: Английский

The role of the brain renin-angiotensin system in Parkinson´s disease DOI Creative Commons
José L. Labandeira‐García, Carmen M. Labandeira, María J. Guerra

et al.

Translational Neurodegeneration, Journal Year: 2024, Volume and Issue: 13(1)

Published: April 15, 2024

Abstract The renin-angiotensin system (RAS) was classically considered a circulating hormonal that regulates blood pressure. However, different tissues and organs, including the brain, have local paracrine RAS. Mutual regulation between dopaminergic RAS has been observed in several tissues. Dysregulation of these interactions leads to renal cardiovascular diseases, as well progression neuron degeneration major brain center dopamine/angiotensin interaction such nigrostriatal system. A decrease function induces upregulation angiotensin type-1 (AT1) receptor activity, leading recovery dopamine levels. AT1 overactivity neurons microglial cells upregulates cellular NADPH-oxidase-superoxide axis Ca 2+ release, which mediate key events oxidative stress, neuroinflammation, α-synuclein aggregation, involved Parkinson's disease (PD) pathogenesis. An intraneuronal antioxidative/anti-inflammatory counteracts effects pro-oxidative overactivity. Consistent with this, an imbalance activity towards pro-oxidative/pro-inflammatory substantia nigra striatum animal models high vulnerability degeneration. Interestingly, autoantibodies against angiotensin-converting enzyme 2 receptors are increased PD patients contribute blood–brain barrier (BBB) dysregulation pro-inflammatory upregulation. Therapeutic strategies addressed modulation RAS, by blockers (ARBs) and/or activation antioxidative (AT2, Mas receptors), may be neuroprotective for individuals risk developing or prodromal stages reduce disease.

Language: Английский

Citations

7

The potential role of brain renin‐angiotensin system in the neuropathology of Parkinson disease: Friend, foe or turncoat? DOI Creative Commons
Zainah Al‐Qahtani, Hayder M. Al‐kuraishy, Ali I. Al‐Gareeb

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(12)

Published: June 1, 2024

Abstract Parkinson disease (PD) is one of the most common neurodegenerative diseases brain. Of note, brain renin‐angiotensin system (RAS) intricate in PD neuropathology through modulation oxidative stress, mitochondrial dysfunction and neuroinflammation. Therefore, RAS by angiotensin receptor blockers (ARBs) angiotensin‐converting enzyme inhibitors (ACEIs) may be effective reducing risk neuropathology. It has been shown that all components including peptides enzymes are present different areas. Brain plays a critical role regulation memory cognitive function, controlling central blood pressure. However, exaggerated implicated pathogenesis PD. Two well‐known pathways recognized including; classical pathway which mainly mediated AngII/AT1R detrimental effects. Conversely, non‐classical mostly ACE2/Ang1‐7/MASR AngII/AT2R beneficial effects against Exaggerated affects viability dopaminergic neurons. fundamental mechanism was not fully elucidated. Consequently, purpose this review to disclose mechanistic In addition, we try revise how ACEIs ARBs can developed for therapeutics

Language: Английский

Citations

7

Identification of key genes and signaling pathway in the pathogenesis of Huntington's disease via bioinformatics and next generation sequencing data analysis DOI Creative Commons
Basavaraj Vastrad, Chanabasayya Vastrad

Egyptian Journal of Medical Human Genetics, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 4, 2025

Abstract Background Huntington's disease (HD) could cause progressive motor deficits, psychiatric symptoms, and cognitive impairment. With the increasing use of pharmacotherapies theoretically target neurotransmitters, incidence HD is still not decreasing. However, molecular pathogenesis have been illuminate. It momentous to further examine HD. Methods The next generation sequencing dataset GSE105041 was downloaded from Gene Expression Omnibus (GEO) database. Using DESeq2 in R bioconductor package screen differentially expressed genes (DEGs) between samples normal control samples. ontology (GO) term REACTOME pathway enrichment were performed on DEGs. Meanwhile, using Integrated Interactions Database (IID) database Cytoscape software construct protein–protein interaction (PPI) network module analysis, identify hub with highest value node degree, betweenness, stress closeness scores. miRNA-hub gene regulatory TF-hub constructed analyzed. Receiver operating characteristic curves analysis for diagnostic genes. Results We identified 958 DEGs, consisting 479 up regulated DEGs down GO terms analyses by g:Profiler online results revealed that mainly enriched multicellular organismal process, developmental signaling GPCR MHC class II antigen presentation. Network Analyzer plugin PPI network, LRRK2, MTUS2, HOXA1, IL7R, ERBB3, EGFR, TEX101, WDR76, NEDD4L COMT selected as Hsa-mir-1292-5p, hsa-mir-4521, ESRRB SREBF1 are potential biomarkers predicted be associated Conclusion This study investigated key pathways interactions its complications, which might help reveal correlation complications. current investigation captured prediction, follow-up biological experiments enforced validation.

Language: Английский

Citations

0

Therapeutic Efficacy of Small Extracellular Vesicles Loaded with ROCK Inhibitor in Parkinson’s Disease DOI Creative Commons

Candy Carbajal,

Myosotys Rodriguez, Florida Owens

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(3), P. 365 - 365

Published: March 13, 2025

Background/Objectives: Parkinson’s disease (PD) is a rapidly growing neurological disorder in the developed world, affecting millions over age of 60. The decline motor functions occurs due to progressive loss midbrain dopaminergic neurons, resulting lowered dopamine levels and impaired muscle function. Studies show defective mitochondrial autophagy (or “mitophagy”) links PD. Rho-associated coiled-coil containing protein kinases (ROCK) 1 ROCK2 are serine/threonine kinases, their inhibition can enhance neuroprotection PD by promoting mitophagy. Methods: We examine effects ROCK inhibitor SR3677, delivered via macrophage-derived small extracellular vesicles (sEVs) Parkin Q311X(A) mouse models. sEVs with administered intranasally, increased mitophagy gene expression, reduced inflammatory factors, elevated brain tissues. Results: expression decreased, showing drug’s inhibitory effect. sEV-SR3677 treatment was more effective than drug alone, although sham EVs showed lower effects. This suggests that EV-SR3677 not only activates processes but also promotes degradation damaged mitochondria through autophagy. Mitochondrial functional assays oxygen consumption ex vivo glial cultures revealed significantly improved respiration compared untreated or SR3677-only treated cells. Conclusion: demonstrated efficacy on function model, necessitating further studies explore design challenges mechanisms as mitochondria-targeted therapy for

Language: Английский

Citations

0

Olfactory mucosal mesenchymal stem cell-derived exosome Lnc A2M-AS1 ameliorates oxidative stress by regulating TP53INP1-mediated mitochondrial autophagy through interacting with IGF2BP1 in Parkinson’s diseases DOI Creative Commons
Jiangshan Zhang, Chuang Wang, Guoshuai Yang

et al.

Cell Biology and Toxicology, Journal Year: 2025, Volume and Issue: 41(1)

Published: March 20, 2025

Abstract Background Exosome Lnc A2M-AS1 from olfactory mucosa mesenchymal stem cells (OM-MSCs) can ameliorate oxidative stress by improving mitophagy in cardiomuscular cells; however, it remains unclear whether this effect exists the brain tissues of patients with Parkinson’s disease (PD). Methods OM-MSC–Exosomes were isolated and verified based on morphology specific biomarkers. The effects OM-MSC-Exo mitochondrial autophagy, stress, lncRNA detected MPP + -treated HT22 cells. OM-MSC-Exos autophagy an MPTP-induced Parkinson's (PD) model C57BL/6 mice. interaction between IGF2BP1, A2M-AS1, TP53INP1 was assessed via RNA pull-down/RNA Immunoprecipitation stability assays. lnc IGF2BP1/TP53INP1-mediated PD mouse models. Results Exosomes found to be rich A2M-AS1. proved able induced regulates enhancing In addition, through mediated expression binding IGF2BP1. Furthermore, treatment improved symptoms ameliorated Conclusion Collectively, OM-MSC-derived exosomes targeting IGF2BP1 induce stress. could potentially serve as promising candidates for new methods PD. Graphical

Language: Английский

Citations

0