Oxidative Stress and the NLRP3 Inflammasome: Focus on Female Fertility and Reproductive Health

Cells, Journal Year: 2025, Volume and Issue: 14(1), P. 36 - 36

Published: Jan. 2, 2025

Chronic inflammation is increasingly recognized as a critical factor in female reproductive health; influencing natural conception and the outcomes of assisted technologies such vitro fertilization (IVF). An essential component innate immunity, NLR family pyrin domain-containing 3 (NLRP3) inflammasome one major mediators inflammatory responses, its activation closely linked to oxidative stress. This interaction contributes decline oocyte quality, reduced potential, impaired embryo development. In ovarian milieu, stress NLRP3 interact intricately, their combined effects on competence are significant. The aims this review examine these molecular mechanisms explore therapeutic strategies targeting activity, with goal enhancing fertility improving clinical health.

Language: Английский

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Impact of Reductive Stress on Human Infertility: Underlying Mechanisms and Perspectives

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11802 - 11802

Published: Nov. 2, 2024

Antioxidants have a well-established effect on general health and are essential in preventing oxidative damage to cells by scavenging free radicals. Free radicals thought be neutralized these substances, which include polyphenols, β-carotene, vitamins C E, reducing cellular damage. On the other hand, recent data indicates that consuming excessive amounts of antioxidants may side effects. Apoptosis cell signaling two beneficial physiological processes affected supplementation. Other negative effects paradoxical enhancement stress unbalanced redox potential. Overdosing particular has been associated with increased medication interactions, cancer progression, fatality risks. Additionally, complex impacts they fertility might both useful adverse, depending quantity duration usage. This review delves into dual role emphasizes importance employing moderation. Antioxidant overconsumption disrupt balance necessary for normal sperm oocyte function, is one potential males females also investigated. Although modest usage generally safe useful, high levels can upset hormonal balance, impair motility, negatively impact outcomes assisted reproductive technologies (ART). The findings emphasize need use antioxidant supplements balanced way, further research optimize their treatments, supporting avoid adverse

Language: Английский

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Histological, immunohistochemical and serological investigations of the ovary during follicular phase of estrous cycle in Saidi sheep

BMC Veterinary Research, Journal Year: 2024, Volume and Issue: 20(1)

Published: March 9, 2024

Abstract Background Saidi sheep are the most abundant ruminant livestock species in Upper Egypt, especially Assiut governorate. Sheep one of animals raised for food Egypt. They can convert low-quality roughages into meat and milk addition to producing fiber hides therefore; great opportunity exists enhance their reproduction. breed is poorly known terms So this work was done give more information on some hormonal, oxidative, blood metabolites parameters histological, histochemical immunohistochemical investigations ovary during follicular phase estrous cycle. The present study conducted 25 healthy ewes serum analysis 10 histological assessment aged 2 5 years weighted (38.5 ± 2.03 kg). Results cycle characterized by presence ovarian follicles different stages development atresia regressed corpus luteum. Interestingly, apoptosis tissue oxidative markers play a crucial role luteum regression. prominent features were mature antral (Graafian) preovulatory as well increased level markers. Here we new schematic sequence describing types. We also clarified that these pictures influenced factors characterizes sheep. Conclusion This helps understanding reproduction which assist improving reproductive outcome These findings increasingly important implementation genetic improvement program utilizing advanced techniques synchronization, artificial insemination embryo transfer.

Language: Английский

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The effects of melatonin on follicular oxidative stress and art outcomes in women with diminished ovarian reserve: a randomized controlled trial

Journal of Ovarian Research, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 8, 2025

To investigate the impact of Melatonin on follicular oxidative stress and assisted reproductive technology (ART) outcomes in women with diminished ovarian reserve (DOR). We put 68 DOR who were going through ART into a randomized controlled trial. Starting fifth day their menstrual cycle, we gave them either 3 mg or placebo every before stimulating ovaries. obtained fluid during oocyte retrieval, assessed it for indicators, documented outcomes. administration markedly enhanced quantity oocytes retrieved, fertilization rates, embryo quality. In addition, changed markers stress, specifically levels reduced glutathione (rGSH) total antioxidant capacity (TAC). The group exhibited significantly elevated biochemical pregnancy rates. may improve quality help low reserves, possibly by lowering follicles.

Language: Английский

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A Retrospective Cohort Study Investigating the Effect of Intraovarian Platelet-Rich Plasma Therapy on the Oxidative State of Follicular Fluid in Women with Diminished Ovarian Reserve

Chonnam Medical Journal, Journal Year: 2025, Volume and Issue: 61(1), P. 46 - 46

Published: Jan. 1, 2025

This study hypothesized that oxidative stress in women with diminished ovarian reserve (DOR) can influence vitro fertilization (IVF) outcomes. Therefore, we investigated the effect of intraovarian platelet-rich plasma (PRP) administration on state follicular fluid DOR patients. Following follow-up a retrospective cohort study, was isolated from twenty-five The total antioxidant capacity (TAC), catalase (CAT), and superoxide dismutase (SOD) activities, oxidant status (TOS), malondialdehyde (MDA) levels were measured before after PRP using colorimetric methods. estradiol (E2), anti-mullerian hormone (AMH), luteinizing (LH), follicle-stimulating (FSH) at baseline two menstrual cycles. quantity quality oocytes embryos assessed both stages. injection, an increase TAC levels, elevated CAT SOD decline TOS MDA content observed. AMH E2 also increased post-treatment. Additionally, relative number observed single injection. Intraovarian injection improves potentially enhances IVF outcomes women.

Language: Английский

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Redox profiling of preovulatory follicular fluid in the donkey is species-specific, and contributes to modulate sperm function

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 23, 2025

Language: Английский

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The Potential Protective Role of Mitochondrial Haplogroup R in Ovarian Response: An Exploratory Study

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2513 - 2513

Published: March 11, 2025

An investigation of the mtDNA haplogroup in 96 Taiwanese women with diminished ovarian response (DOR) and normal (NOR) showed that only R is less likely to experience DOR than other haplogroups. When analyzing relationship between age mitochondria-related markers (mtDNA copy number, ROS levels, telomere length), it was observed levels length exhibited age-dependent changes, number retrieved oocytes decreased age. However, haplogroup, these remained stable did not show significant changes Additionally, decline age, suggesting a unique protective effect associated this haplogroup. Our study supports notion may serve as biomarker for infertility women.

Language: Английский

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Redox Balance and Inflammatory Response in Follicular Fluids of Women Recovered by SARS-CoV-2 Infection or Anti-COVID-19 Vaccinated: A Combined Metabolomics and Biochemical Study

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8400 - 8400

Published: Aug. 1, 2024

To date, not many studies have presented evidence of SARS-CoV-2 infecting the female reproductive system. Furthermore, so far, no effect administration anti-COVID 19 vaccines has been reported to affect quality oocytes retrieved from women who resorted assisted reproduction technology (ART). The FF metabolic profiles had infected by before IVF treatments or after COVID-19 vaccination were examined 1H NMR. Immunochemical characterization proteins and cytokines involved in redox inflammatory pathways was performed. increased expression SOD2 NQO1, lack alteration IL-6 CXCL10 levels, as well CD39, suggested that, both sharing similar molecular mechanisms proceeding along different routes, balance is controlled vaccinated recovered compared controls. lower amount metabolites known proinflammatory activity, i.e., TMAO lipids, further supported biochemical results, suggesting that microenvironment guarantee oocyte does compromise outcome ART. In terms number blastocysts obtained ICSI pregnancy rate, results are also comforting.

Language: Английский

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Supplementation with L-kynurenine during in vitro maturation improves bovine oocytes developmental competence through its antioxidative action

Theriogenology, Journal Year: 2024, Volume and Issue: 233, P. 53 - 63

Published: Nov. 20, 2024

Language: Английский

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Unravelling the Signature Follicular Fluid Metabolites in Dairy Cattle Follicles Growing Under Negative Energy Balance: An In Vitro Approach

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12629 - 12629

Published: Nov. 25, 2024

The astringent selection criteria for milk-oriented traits in dairy cattle have rendered these animals prone to various metabolic disorders. Postpartum lactational peak and reduced feed intake lead negative energy balance cattle. As a compensatory mechanism, start mobilizing fat reserves meet the demand vital body functions. Consequently, diminished glucose concentrations elevated ketone levels poor ovarian function. impaired follicular development subpar oocyte quality diminish conception rates, which poses significant economic repercussions. Follicular fluid is integral processes of growth development. Hence, present study was performed identify potential alterations metabolites under vitro culture conditions mimicking balance. Our results revealed nine distinct exhibiting differential expression differentially expressed were predominantly associated with pathways related amino acid metabolism, lipid signal transduction mechanisms, membrane transport, alongside other biological processes. identified signature may be further validated determine fitness subjected fertilization or embryo production from slaughterhouse source ovaries.

Language: Английский

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