Pyrroloquinoline Quinone Improved Boar Sperm Quality via Maintaining Mitochondrial Function During Cryopreservation

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 102 - 102

Published: Jan. 16, 2025

Due to oxidative damage and mitochondrial dysfunction, boar semen cryopreservation remains a significant challenge. This study investigates the effects of pyrroloquinoline quinone (PQQ), mitochondrial-targeted antioxidant, on post-thaw sperm quality during cryopreservation. Boar was diluted in freezing extender containing different concentrations PQQ (0, 10, 100, 1000, 10,000 nM). After freezing-thawing, motility, viability, acrosome integrity, activity, adenosine triphosphate (ATP) levels, DNA malondialdehyde (MDA) reactive oxygen species (ROS) superoxide dismutase (SOD) transcription proteins fertilization capacity were assessed. The results show that 1000 nM supplementation significantly enhanced integrity compared control (p < 0.05). Additionally, increased membrane potential (MMP) ATP while decreasing MDA ROS reducing Furthermore, levels mitochondrial-encoded elevated group Interestingly, showed higher binding rate oviductal epithelial cells zona pellucida (ZP), indicating potential. These findings suggest use mitochondria-target PQQ, can improve via its reduce stress protect function.

Language: Английский

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Polydopamine-based nano-protectant for prolonged boar semen preservation by eliminating ROS and regulating protein phosphorylation via D2DR-mediated cAMP/PKA signaling pathway

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 27, 2025

Language: Английский

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BGP-15 improves quality of goat sperm by mitigating oxidative stress during cryopreservation

Cryobiology, Journal Year: 2025, Volume and Issue: 119, P. 105232 - 105232

Published: March 14, 2025

Language: Английский

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Ellagic acid maintains post-thaw goat sperm quality via protecting mitochondrial function from ROS damage

Cryobiology, Journal Year: 2025, Volume and Issue: 119, P. 105231 - 105231

Published: March 24, 2025

Language: Английский

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Current study on Pyrroloquinoline quinone (PQQ) therapeutic role in neurodegenerative diseases

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: April 15, 2025

Language: Английский

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Pyrroroquinoline Quinone (PQQ) Improves the Quality of Holstein Bull Semen during Cryopreservation

Animals, Journal Year: 2024, Volume and Issue: 14(20), P. 2940 - 2940

Published: Oct. 11, 2024

Cryopreserved semen is extensively utilized in the artificial insemination (AI) of domestic animals; however, suboptimal conception rates due to oxidative damage following AI continue pose a challenge. The present study investigated effects Pyrroroquinoline Quinone (PQQ), novel antioxidant, on quality Holstein bulls during cryopreservation, as well its potential molecular mechanisms. Semen samples were diluted with varying concentrations PQQ (0, 50 μmol/L, 100 150 μmol/L) prior cryopreservation. Following freeze-thaw process, comprehensive evaluation was conducted assess sperm motility, plasma membrane integrity, acrosome and levels reactive oxygen species (ROS), malondialdehyde (MDA), adenosine triphosphate (ATP). Western blot analysis employed examine proteins including PGAM2, CAPZB, CAT, SOD1, GPX1. Notably, inclusion μmol/L significantly enhanced integrity post freeze-thawing (

Language: Английский

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Culture media with antioxidants improved fertilization, preimplantation embryo development and clinical outcomes of advanced age patients

Reproductive BioMedicine Online, Journal Year: 2024, Volume and Issue: 50(1), P. 104415 - 104415

Published: Aug. 23, 2024

Language: Английский

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