Published: Jan. 1, 2024
Language: Английский
Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 1, 2024
Heat stress (HS) has become a major factor limiting crop yields worldwide. HS inhibits plant growth by ROS accumulation, and NADPH oxidases (Rbohs) are producers in plants. Here, we show that CRISPR/Cas knockout of the OsRbohB (OsRbohB-KO) significantly increased rice tolerance to imposed at various different stages. We produced OsRbohB-KO OsRbohB-overexpression (OsRbohB-OE) lines japonica cultivar, Nipponbare. Compared with nontransgenic wild-type (WT) plants, showed significant increase chlorophyll contents (5.2%-58.0%), (48.2%-65.6%) grain yield (8.9%-20.5%), while reducing HS-induced accumulation seeds (21.3%-33.0%), seedlings (13.0%-30.4%), anthers (13.1%-20.3%) grains (9.7%-22.1%), under conditions. Analysis components revealed plants was due starch synthetase activity, spikelets per panicle (2.0%-9.3%), filled (4.8%-15.5%), percentage (2.4%-6.8%) 1000-grain weight (2.9%-7.4%) conditions during reproductive stage. Grain milling appearance quality, content were also mature Furthermore, upregulated expression levels heat shock-related genes, OsHSP23.7, OsHSP17.7, OsHSF7 OsHsfA2a, long-term Conversely, OsRbohB-OE resulted phenotypes opposite most cases. Our results suggest suppression provides an effective approach for alleviating damage improving quality
Language: Английский
Citations
4
Applied Surface Science, Journal Year: 2025, Volume and Issue: unknown, P. 162572 - 162572
Published: Feb. 1, 2025
Language: Английский
Citations
0
Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 291, P. 117889 - 117889
Published: Feb. 1, 2025
The hormesis effect has gradually become a research focus in environmental science and toxicology due to its unique phenomenon of low-concentration stimulation high-concentration inhibition. combined toxicity antibiotics their regulatory effects on microorganisms are areas that warrant significant attention. In this study, doxycycline hydrochloride (DXC), sulfamethoxazole (SMX) sulfamethazine (SMZ) were used as the objects, Scenedesmus obliquus was indicator organism study action laws at different exposure times (4d 8d). toxic antibiotic mixtures with comprehensively evaluated from aspects growth inhibition, photosynthesis index antioxidant enzyme activity. results showed 11 rays produced effect, value 8th day greater than 4th day. Through prediction toxicity, it found type intensity affected by mixture composition, time concentration. Hormesis is caused regulation photosynthesis, activation defense system, gene expression, change cell membrane permeability. This provides theoretical basis for understanding effect.
Language: Английский
Citations
0
Journal of the Science of Food and Agriculture, Journal Year: 2025, Volume and Issue: unknown
Published: April 6, 2025
Abstract Zinc (Zn) is a vital micronutrient required for optimal plant growth and soil fertility. Its use in the form of nanoparticles (NPs) has gained significant attention agricultural applications. Green synthesized Zn‐based NPs offer an eco‐friendly solution to several conventional problems agriculture. Several plants, bacteria, fungi yeast have shown potential fabricating Zn that can provide environmentally friendly solutions agriculture approach aligned with sustainable practices, reducing dependency on harmful agrochemicals. act as promoters, enhance crop yield, promote resilience abiotic stressors are efficient protection agents. Their role smart delivery system, enabling targeted controlled release agrochemicals, further signifies their Because requires repeated applications hence, toxicological aspects cannot be ignored. reported cause phytotoxicity, including root damage, physiological biochemical disturbances, genotoxic effects. Furthermore, exposure poses risks microbiota, aquatic terrestrial organisms potentially impacting ecosystem. The green synthesis promising aspect advancing by agrochemical improving productivity. diverse protectants systems emphasize potential. However, essential ensure standardization doses safe effective use. Further research would help address such concerns developing viable modern © 2025 Society Chemical Industry.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 6877 - 6877
Published: June 22, 2024
Higher-fungi xylotrophic basidiomycetes are known to be the reservoirs of bioactive metabolites. Currently, a great deal attention has been paid exploitation mycelial fungi products as an innovative alternative in crop protection. No data exist on mechanisms behind interaction between mushrooms’ glycopolymeric substances and plants. In this study, effects basidiomycete metabolites morphophysiological biochemical variables wheat plants have explored. Wheat (Triticum aestivum L. cv. Saratovskaya 29) seedlings were treated with extracellular polysaccharides (EPSs) isolated from submerged cultures twenty strains assigned 13 species 8 genera. The EPS solutions at final concentrations 15, 40, 80 mg/L applied followed by their growth for 10 days. plant samples, biomass, length coleoptile, shoot root, root number, rate lipid peroxidation malondialdehyde concentration, content hydrogen peroxide, total phenols measured. peroxidase superoxide dismutase activity defined. Most preparations improved biomass yields, well morphological parameters examined. application enhanced activities antioxidant enzymes decreased oxidative damage lipids. Judging its overall effect indices redox system plants, concentration 40 shown most beneficial compared other concentrations. This study proves that novel bioformulations based mushroom EPSs can developed effective antioxidative response. Phytostimulating properties found give grounds consider produced active component capable inducing responses stress.
Language: Английский
Citations
2
ACS Food Science & Technology, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 8, 2024
Many natural products (NPs) exhibit strong antioxidant activity, making them valuable for various applications. In this context, the emergence of deep eutectic solvents (DESs) is expected to help address growing demand innovative, efficient, and more sustainable methods NPs extraction. DESs are designer that provide higher yields enhance activity extracted phytochemicals compared with conventional volatile organic solvents. Antioxidant naturally occurring molecules can also form DESs, either as hydrogen bond donors (HBD) or acceptors (HBA), creating DES (NADES) inherent activity. Thus, containing exert synergistic effects, entire system a promising active ingredient food industry The synergy arises from enhanced solubilization stabilization in through bonding, ionic interactions, van der Waals forces. Collectively, enable effectively neutralize oxidants (such ROS free radicals) regulate their reactions other molecules, achieving up 3-fold increase extracts regular vitro. This Account overviews wide range resources using DESs. activities HBA, HBD, along advantageous coupling extracts, thoroughly discussed. Finally, we envisage future directions potential applications harnessing collective guide advances chemistry.
Language: Английский
Citations
2
Agricultural Research, Journal Year: 2024, Volume and Issue: 13(4), P. 660 - 675
Published: June 28, 2024
Language: Английский
Citations
1
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Aug. 22, 2024
Abstract The present study focused on the impact of infection with tobacco mosaic virus (TMV). Specifically, changes in phytochemicals and gene activity related to pathogenesis-related phenylpropanoid pathway genes tomato plants ( Solanum lycopersicum L.) during a period 2–14 days post-inoculation (dpi). According TEM investigation coat protein sequence analysis, purified TMV Egyptian AM isolate (PP133743) has rod-shaped structure diameter around 110 nm. RT-qPCR analysis revealed that PR-1 showed an initial increase after infection, as seen time-course analysis. In contrast, PR-2 was consistently elevated throughout suggesting stronger reaction suppressing PAL expression at 6 14 dpi. levels HQT CHS transcripts exhibited alternating patterns up-regulation down-regulation different time intervals. HPLC GC–MS control- TMV-infected extracts phenolic, flavonoid, fatty acid compounds were increased (such naringenin, rutin, flavone, ferulic acid, pyrogallol) or significantly decreased salicylic chlorogenic acid) infection. ability inhibit most polyphenolic could potentially accelerate viral life cycle. Consequently, focusing enhancing such suppressed may be critical for developing plant management strategies.
Language: Английский
Citations
1
Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 423, P. 136858 - 136858
Published: Oct. 30, 2024
Language: Английский
Citations
1
Journal of soil science and plant nutrition, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 19, 2024
Language: Английский
Citations
1