Bioactive Compounds Protect Mammalian Reproductive Cells from Xenobiotics and Heat Stress-Induced Oxidative Distress via Nrf2 Signaling Activation: A Narrative Review

Antioxidants, Journal Year: 2024, Volume and Issue: 13(5), P. 597 - 597

Published: May 13, 2024

Oxidative stress occurs when there is an imbalance between the production of reactive oxygen species (ROS) and body’s antioxidant defenses. It poses a significant threat to physiological function reproductive cells. Factors such as xenobiotics heat can worsen this stress, leading cellular damage apoptosis, ultimately decreasing efficiency. The nuclear factor erythroid 2–related 2 (Nrf2) signaling pathway plays crucial role in defending against oxidative protecting cells via enhancing responses. Dysregulation Nrf2 has been associated with infertility suboptimal performance mammals. Recent advancements therapeutic interventions have underscored critical mitigating restoring functional integrity In narrative review, we delineate harmful effects xenobiotic-induced on explain how provides protection these challenges. studies shown that activating using various bioactive compounds ameliorate distress apoptosis mammalian By comprehensively analyzing existing literature, propose key target for caused by exposure xenobiotic stress. Additionally, based synthesis findings, discuss potential therapies focused improve

Language: Английский

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In silico and experimental potentials of 6-shogaol and meglumine antimoniate on Leishmania major: multiple synergistic combinations through modulation of biological properties

Immunologic Research, Journal Year: 2024, Volume and Issue: 72(6), P. 1313 - 1326

Published: Aug. 18, 2024

Language: Английский

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Targeting Nrf2/KEAP1 signaling pathway using bioactive compounds to combat mastitis

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 7, 2025

Mastitis is a common inflammation of mammary glands that has significantly impact on dairy production and animal health, causing considerable economic burdens worldwide. Elevated reactive oxygen species (ROS) followed by oxidative stress, apoptosis, inflammatory changes suppressed immunity are considered the key biomarkers observed during mastitis. The Nrf2/KEAP1 signaling pathway plays critical role in regulating antioxidant responses cellular defense mechanisms. When activated bioactive compound treatment, Nrf2 translocates to nucleus induces expression its target genes exert responses. This reduces pathogen-induced stress inhibiting NF-kB glands, one prominent pro-inflammatory pathway. Here, we summarize recent studies highlight therapeutic potential prevention treatment Collectively this review article aims explore compounds mitigating mastitis targeting

Language: Английский

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Long-term consumption of hydrogen-rich water provides hepatoprotection by improving mitochondrial biology and quality control in chronically stressed mice

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0317080 - e0317080

Published: Feb. 14, 2025

Background Chronic stress has emerged as a prevalent facet of contemporary existence, significantly jeopardizing overall bodily health. The liver, pivotal organ responsible for metabolic equilibrium, is particularly vulnerable to its adverse effects. This study delves into the hepatoprotective properties extended consumption HRW in mice subjected chronic stress. Methods Mice via CUMS and administration seven months underwent liver pathological examination. Key function indicators (AST, ALT), oxidative markers (SOD, CAT, GSH), related lipid peroxidation ferroptosis (MDA, Fe) were measured using standard kits. ELISA determined corticosterone 4-HNE levels. Immunofluorescence evaluated ROS, Nrf2, apoptosis tissues. Western blotting analyzed (GPX4, SLC7A11, HO-1, Nrf2), (Bax, Bcl-2, Cytc, Caspase-3, Caspase-8), mitochondrial biogenesis (Nrf1, PGC-1α, Tfam), quality control (Drp1, Fis1, Mfn1, Mfn2, OPA1, PINK1, Parkin, LC3 I/II). Results findings indicate noteworthy improvement health among exposed HRW, evidenced by histological analysis. Furthermore, exhibited hepatoprotection, normalization AST ALT Mechanistically, our results that elevates levels SOD, GSH, while effectively clearing ROS within mitochondria. It was observed led regulation expression proteins, consequently improving increasing ATP production. decreased Bax, Caspase-8 levels, Bcl-2. Additionally, reduced MDA alleviating through Nrf2/HO-1 pathway, upregulating GPX4 SLC7A11. By mitigating hepatocyte death aforementioned mechanisms, fulfills crucial role safeguarding Conclusions reveals long-term hydrogen-rich water (HRW) provides significant hepatoprotection under normalizes enzyme enhances antioxidant capacity, reduces ferroptosis. improves biogenesis, function, production, attenuates modulating proteins. Behavioral tests show alleviates stress-induced anxiety exploratory behavior. These suggest promising non-invasive intervention preventing treating stress-related disorders targeting dysfunction.

Language: Английский

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The Regulation of γ-Aminobutyric Acid on Antioxidative Defense Response of Pacific Oyster upon High-Temperature Stress

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 222 - 222

Published: Feb. 15, 2025

Recent studies have found that high temperatures cause oxidative stress and even mass mortality in Pacific oysters (Crassostrea gigas). The role of γ-aminobutyric acid (GABA) improving antioxidative defense aquatic animals is increasingly interest. In the present study, to high-temperature was examined, regulation GABA on further investigated. Following 6 h exposure 28 °C seawater, a significant increase mRNA expression levels nuclear factor-E2-related factor 2 (Nrf2), superoxide dismutase (SOD), catalase (CAT), as well activities SOD CAT, observed gill, compared those at 0 h. An glutamate decarboxylase (GAD), receptor (GABAAR-α GABABR-B) levels, contents were also detected after Furthermore, CAT significantly upregulated treatment, while decreased either GAD inhibitor or treatment under stress. Meanwhile, enhanced effects antioxidant enzyme reduced when Nrf2 inhibited by ML385, accompanied an MDA content. After stress, with group, GSK-3β treatment. elevation attenuated AKT Collectively, first activated receptors then increased content AKT/GSK-3β pathways protect against damage upon results offer new insights for understanding mechanisms neuroendocrine system molluscs.

Language: Английский

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Influence of probiotic strains on growth performance, carcass characteristics and seasonal changes of growing New Zealand white rabbits

Tropical Animal Health and Production, Journal Year: 2024, Volume and Issue: 56(8)

Published: Oct. 8, 2024

Language: Английский

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Transcriptome Analysis Reveals Sertoli Cells Adapting Through Redox and Metabolic Pathways Under Heat Stress in Goats

Genes, Journal Year: 2024, Volume and Issue: 15(12), P. 1582 - 1582

Published: Dec. 9, 2024

Background/Objectives: Climate change-induced temperature elevations pose significant challenges to livestock reproduction, particularly affecting testicular function in small ruminants. This study investigates the acute heat-stress response goat Sertoli cells (SCs), aiming elucidate molecular mechanisms underlying heat-induced damage male reproductive tissues. Methods: SCs were isolated from testes of 4-month-old black goats and exposed heat stress (44 °C for 2.5 h). We employed transcriptome sequencing, CCK-8 assay, electron microscopy, ROS measurement, autophagy detection, Western blot analysis, lactate concentration measurement. Bioinformatics analyses including Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway, protein–protein interaction network performed on data. Results: Heat significantly reduced SC viability, induced oxidative autophagy, altered gene expression profiles. identified 1231 differentially expressed genes, with enrichment membrane-related processes metabolic pathways. Metabolism-related PKLR, ACOT11, LPCT12, downregulated. Protein–protein analysis revealed ten hub genes potentially crucial response: HSP90AA1, HSPA5, BAG3, IGF1, HSPH1, IL1A, CCL2, CXCL10, ALB, CALML4. Conclusions: provides comprehensive insights into stress. The pathways, those related metabolism response, offer potential targets developing strategies mitigate effects reproduction. These findings contribute our understanding climate change impacts animal husbandry may inform development resistant lines.

Language: Английский

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Evaluating the Antioxidant and Anticancer Activity of <i>Saccharum spontaneum</i> Linn Flower Extract in Hepatocellular Carcinoma: An <i>In Vitro</i> and <i>In Vivo</i> Study

Journal of Natural Remedies, Journal Year: 2024, Volume and Issue: unknown, P. 2493 - 2503

Published: Dec. 5, 2024

Background: Advanced Hepatocellular Carcinoma (HCC) is a devastating disease with poor survival rate, emphasising the urgent need for novel and effective therapeutic agents. Saccharumspontaneum Linn (S. spon), member of Poaceae family, has garnered attention due to its diverse pharmacological benefits, including antibacterial, antioxidant anti-inflammatory properties. Aim: This study aims investigate phytochemical composition S. spon flower extract evaluate anticancer potential against HCC. Methods: The activity Methanolic Extract (ME) flowers was assessed using DPPH Ferric Reducing Antioxidant Power (FRAP) assays. efficacy evaluated MTT assay on HepG2 cell lines. An in vivo also conducted diethyl nitrosamine-induced hepatocellular carcinoma animal model. Results: results showed significant FRAP revealed dose- time-dependent decrease viability, an IC50 value 25.5 µM/mL. Furthermore, ME exhibited notable cytotoxic effects mitigated liver injury model, as evidenced by decreased enzyme levels (p < 0.05). Conclusion: demonstrates that possesses antitumor potential, making it promising candidate treatment hepatic cancer. findings suggest inhibits cancer growth induces programmed death, warranting further investigation applications.

Language: Английский

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Higher oxidative balance score is associated with lower female infertility: a cross-sectional study

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 4, 2024

An imbalance of the pro-oxidant and antioxidant profiles in body contributes to development diseases, including female infertility.

Language: Английский

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