Sterile inflammation induced by respirable micro and nano polystyrene particles in the pathogenesis of pulmonary diseases DOI
A. Laganà, Giuseppa Visalli, Alessio Facciolà

et al.

Toxicology Research, Journal Year: 2024, Volume and Issue: 13(5)

Published: Sept. 2, 2024

Abstract Sterile inflammation is involved in the lung pathogenesis induced by respirable particles, including micro- and nanoplastics. Their increasing amounts ambient indoor air pose a risk to human health. In two cell lines (A549 THP-1) we assessed proinflammatory behavior of polystyrene nanoplastics (nPS) microplastics (mPS) (Ø 0.1 1 μm). Reproducing environmental aging, addition virgin, cells were exposed oxidized nPS/mPS. To study response monocytes inflammatory signal transmitted A549 through release soluble factors (e.g. alarmins cytokines), THP-1 also supernatants previously nPS/mPS-treated A549. After dynamic-light-scattering (DLS) analysis protein measurements for assessment corona nPS/mPS, real-time PCR enzyme-linked-immunosorbent (ELISA) assays performed cells. The pro-inflammatory effects v- ox-nPS/mPS attested imbalance Bax/Bcl-2 ratio A549, which was able trigger cascade, inhibiting immunologically silent apoptosis. involvement NFkB confirmed overexpression p65 after exposure ox-nPS ox-mPS. fast higher levels IL-1β, only cells, underlined NLPR3 inflammasome activation.

Language: Английский

Paeoniflorin Attenuates APAP-Induced Liver Injury via Intervening the Crosstalk Between Hepatocyte Pyroptosis and NETs DOI Open Access
Yousong Zhu,

Yaqin Yang,

Dan‐dan Ruan

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1493 - 1493

Published: Feb. 11, 2025

(1) Liver injury caused by an overdose of acetaminophen (APAP) represents a major public health concern. Paeoniflorin (PF) has been reported to have anti-inflammatory and liver-protective effects, but the underlying mechanisms remain unclear. This study aimed investigate effect PF on crosstalk between pyroptosis NETs in AILI. (2) APAP-treated C57BL/6J mice were used demonstrate protective liver injury. HepG2 dHL-60 cells cultured effects hepatocyte neutrophil extracellular traps (NETs) vitro. Moreover, cell co-culture experiments performed, treated with NETs-depleting agent inhibitor improvement AILI induced through regulating NETs. (3) significantly alleviated Additionally, inhibited expression pyroptosis-related proteins, high-mobility group box 1 (HMGB1), NETs-associated proteins vitro vivo. The demonstrated that not only triggered pyroptosis, also suppressed In depleted neutrophils, level notably decreased, indicating diminished impact PF. Similarly, formation was reduced receiving compared APAP group. Compared DNase I alone, reduction combined serum ALT AST levels decreased from 46.857% 39.927% 44.347% 33.419%, respectively. DSF 45.347% 36.419%, (4) therapeutic Its mechanism involves regulation research substantiates pharmacological promise as intervention for acute

Language: Английский

Citations

0
Interleukin-6 in neuroimmunological disorders: Pathophysiology and therapeutic advances with satralizumab DOI
Xicheng Li, Chongbo Zhao

Autoimmunity Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 103826 - 103826

Published: May 1, 2025

Language: Английский

Citations

0
Sterile inflammation induced by respirable micro and nano polystyrene particles in the pathogenesis of pulmonary diseases DOI
A. Laganà, Giuseppa Visalli, Alessio Facciolà

et al.

Toxicology Research, Journal Year: 2024, Volume and Issue: 13(5)

Published: Sept. 2, 2024

Abstract Sterile inflammation is involved in the lung pathogenesis induced by respirable particles, including micro- and nanoplastics. Their increasing amounts ambient indoor air pose a risk to human health. In two cell lines (A549 THP-1) we assessed proinflammatory behavior of polystyrene nanoplastics (nPS) microplastics (mPS) (Ø 0.1 1 μm). Reproducing environmental aging, addition virgin, cells were exposed oxidized nPS/mPS. To study response monocytes inflammatory signal transmitted A549 through release soluble factors (e.g. alarmins cytokines), THP-1 also supernatants previously nPS/mPS-treated A549. After dynamic-light-scattering (DLS) analysis protein measurements for assessment corona nPS/mPS, real-time PCR enzyme-linked-immunosorbent (ELISA) assays performed cells. The pro-inflammatory effects v- ox-nPS/mPS attested imbalance Bax/Bcl-2 ratio A549, which was able trigger cascade, inhibiting immunologically silent apoptosis. involvement NFkB confirmed overexpression p65 after exposure ox-nPS ox-mPS. fast higher levels IL-1β, only cells, underlined NLPR3 inflammasome activation.

Language: Английский

Citations

3