Ellagic Acid Modulates Necroptosis, Autophagy, Inflammations, and Stress to Ameliorate Nonalcoholic Liver Fatty Disease in a Rat Model

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 1, 2025

ABSTRACT Nonalcoholic fatty liver disease (NAFLD) is considered one of the most common metabolic disorders worldwide. Although pathoetiology NAFLD not fully elucidated, recent evidence suggests involvement stress, inflammation, and programmed death in onset progression disease. This investigation aimed to evaluate effects ellagic acid (EA), a known herbal antioxidant, on high‐fat diet (HFD)‐induced animal model by evaluating status lipid profile, necroptosis (RIPK1, RIPK3, MLKL), autophagy (LC3, ATG5, BECN1), inflammation (TNF‐α, IL‐6, IL‐4, IL‐10), stress (SOD, CAT, GR, GPx, MDA). In this regard, rats were randomly divided into 6 groups as follows: normal controls, HFD (supplemented with high caloric model), EA low dose (HFD 10 mg/kg/day EA), middle 25 50 Rosiglitazone Rosi). After treatment, levels markers related tissue well profiles, oxidative analyzed. It was shown that able improve weight gain profile when compared animals ( p ‐value < 0.001). Moreover, increased level LC3 ATG5 while decreasing BECN 1, RIPK1, MLKL HFD‐induced 0.05). TNF‐α IL‐6 decreased after administration, whereas IL‐4 IL‐10 Furthermore, increase activity SOD, GPx along decrease MDA indicated suppression treatment 0.0001). The current findings may suggest improves via modulation necroptosis, autophagy, stress.

Language: Английский

Pomegranate‐Derived Exosome‐Like Nanovesicles Containing Ellagic Acid Alleviate Gut Leakage and Liver Injury in MASLD

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(4)

Published: April 1, 2025

ABSTRACT Exosome‐like nanovesicles derived from plants (PENs) harbor a spectrum of bioactive compounds, including proteins, lipids, nucleic acids (such as miRNAs and mRNAs), offering therapeutic advantages for variety diseases. This investigation assesses the utility pomegranate‐derived exosome‐like (PNVs) in both preventing treating complications such liver damage increased intestinal permeability found Metabolic Dysfunction‐Associated Steatotic Liver Disease (MASLD). Utilizing Transmission Electron Microscopy (TEM) Nanoparticle Tracking Analysis (NTA), we successfully isolated PNVs characterized their structural properties. Upon administration live model, these were efficiently distributed to critical organs, intestines, demonstrating biocompatibility by avoiding toxic effects. Remarkably, vesicles enriched with ellagic acid, known its strong antioxidant capabilities. In controlled MASLD mouse study, treatment significantly lowered serum endotoxin levels, reinforced barrier functions, altered gut microbiota profile favorably. Furthermore, application reduced oxidative stress presence fibrosis markers normal levels. These results indicate that could be viable option addressing MASLD, enhancing integrity, reducing injury, diminishing through modulation gut‐liver axis.

Language: Английский

Differential effects of ellagic acid on non-alcoholic fatty liver disease in mice: grouped by urolithin A-producing capacity

Food & Function, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Ellagic acid (EA) exhibits protective effects on non-alcoholic fatty liver disease (NAFLD). However, the ability to produce urolithins and health benefits associated with EA consumption differ considerably among individuals. Therefore, different of high-fat high-fructose diet (HFFD)-induced NAFLD, considering variability in urolithin-producing ability, were explored. Our results showed that could effectively reduce body weight, lipid accumulation insulin resistance, improve oxidative stress inflammation NAFLD mice. The metabolomics analysis indicated metabolism disorder induced by HFFD was obviously improved mainly through regulation unsaturated biosynthesis amino metabolism. In particular, improvement effect mice high urolithin A production better than their low counterparts. Moreover, treatment reshaped gut microbiota imbalance caused HFFD. Specifically, compared model group, lower abundances Faecalibaculum (by 95.11%), Ruminococcus_torques_group 208.14%), Clostridium_sensu_stricto_1 449.37%), Ileibacterium 172.64%), while higher Verrucomicrobia Akkermansia 425.0%) observed high-UroA-producing group (p < 0.05). This study provided new insights into EA's anti-NAFLD effectiveness suggested response capacity greatly determined performance alleviating development NAFLD.

Language: Английский