The critical role of X-linked inhibitor of apoptosis protein (XIAP) in tumor development

APOPTOSIS, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Language: Английский

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Oxidative Stress and Redox Imbalance: Common Mechanisms in Cancer Stem Cells and Neurodegenerative Diseases

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 511 - 511

Published: March 29, 2025

Oxidative stress (OS) is an established hallmark of cancer and neurodegenerative disorders (NDDs), which contributes to genomic instability neuronal loss. This review explores the contrasting role OS in stem cells (CSCs) NDDs. Elevated levels reactive oxygen species (ROS) contribute promote tumor initiation progression CSCs, while NDDs such as Alzheimer’s Parkinson’s disease, accelerates death impairs cellular repair mechanisms. Both scenarios involve disruption delicate balance between pro-oxidant antioxidant systems, leads chronic oxidative stress. Notably, CSCs neurons display alterations redox-sensitive signaling pathways, including Nrf2 NF-κB, influence cell survival, proliferation, differentiation. Mitochondrial dynamics further illustrate these differences: enhanced function supports adaptability whereas impairments heighten vulnerability. Understanding common mechanisms OS-induced redox imbalance may provide insights for developing interventions, addressing aging hallmarks, potentially mitigating or preventing both

Language: Английский

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Widely Targeted Metabolomics Reveals the Bioactive Metabolites and Antioxidant Activities of Chinese Yam (Dioscorea opposita Thunb.) Peel

Biomedical Chromatography, Journal Year: 2025, Volume and Issue: 39(5)

Published: April 10, 2025

ABSTRACT Chinese yam ( Dioscorea opposita Thunb. cv. Tiegun) has been utilized in traditional medicine and as a food source for centuries. However, the metabolite profiles antioxidant activities of by‐product peel have not studied sufficiently. Thus, to effectively identify active metabolites peel, we employed UHPLC–MS/MS‐based widely targeted metabolomics on from loessial soil (LPCY) sandy (SPCY). A total 1054 were identified, comprising 379 primary metabolites, 528 secondary 147 other compounds. Notably, multivariate analyses revealed presence 143 differentially accumulated (DAMs) between SPCY LPCY. Linoleic acid metabolism, phenylpropanoid biosynthesis, plant hormone signal transduction, pyruvate sphingolipid metabolism main regulated pathways. The DPPH, ABTS, FRAP assays demonstrated that LPCY significantly higher than those SPCY. Correlation analysis most DAMs, including phenolic acids, lipids, organic amino exhibited significant positive correlations with r ≥ 0.7, p < 0.05). These results indicate promotes accumulation antioxidant‐active Overall, this study suggests peels hold potential rich natural bioactive substances.

Language: Английский

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Optimization of the Antibacterial Activity of a Three-Component Essential Oil Mixture from Moroccan Thymus satureioides, Lavandula angustifolia, and Origanum majorana Using a Simplex–Centroid Design

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(1), P. 57 - 57

Published: Jan. 7, 2025

The rise of antibiotic-resistant pathogens has become a global health crisis, necessitating the development alternative antimicrobial strategies. This study aimed to optimize antibacterial effects essential oils (EOs) from Thymus satureioides, Lavandula angustifolia, and Origanum majorana, enhancing their efficacy through optimized mixtures. utilized simplex-centroid design mixture ratios EOs for maximal antioxidant effectiveness. chemical profiles were analyzed using gas chromatography-mass spectrometry (GC-MS). activity was assessed against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa minimum inhibitory concentration (MIC) tests, while evaluated DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assays. oil mixtures demonstrated potent activity, with MIC values 0.097% (v/v) E. 0.058% S. 0.250% P. aeruginosa. achieving these results included 76% T. 24% O. majorana varying proportions other strains. Additionally, L. angustifolia exhibited strongest IC50 84.36 µg/mL (DPPH), 139.61 (ABTS), surpassing both standard antioxidants like BHT ascorbic acid in assay. successfully demonstrates that can serve as effective natural agents. findings highlight novel approach enhance applications oils, suggesting potential use food preservation biopharmaceutical formulations. optimization strategy offers promising avenue combat antibiotic resistance safety products.

Language: Английский

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Equol Alleviates the In Vitro Aging‐Induced Disruption of Porcine Oocytes

Reproduction in Domestic Animals, Journal Year: 2025, Volume and Issue: 60(1)

Published: Jan. 1, 2025

ABSTRACT Oocyte quality is crucial for determining the subsequent embryo developmental capacity and reproductive outcomes. However, aging detrimental to oocyte quality. Previous studies have demonstrated that soy isoflavones positive effects on performance of female pigs. Equol, primary metabolite isoflavones, renowned its antioxidant properties ability scavenge reactive oxygen species (ROS). potential role equol in reversing aging‐mediated decline has not yet been elucidated. In this study, we treated porcine oocytes with different concentrations (2.5, 5 10 μM) during prolonged vitro culture. Our findings showed led decreased embryonic capacity, indicating We further found disrupted spindle assembly chromosome arrangement, impaired actin polymerisation reduced mitochondrial activity function. Moreover, increased ROS levels; thereafter, DNA damage apoptosis was induced oocytes. Interestingly, treatment 2.5 μM process significantly mitigated above‐mentioned defective parameters finally improved development rates. Collectively, these results imply benefits attenuating defects

Language: Английский

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Recent Issues in the Development and Application of Targeted Therapies with Respect to Individual Animal Variability

Animals, Journal Year: 2025, Volume and Issue: 15(3), P. 444 - 444

Published: Feb. 6, 2025

This literature review explores the impact of molecular, genetic, and environmental factors on efficacy targeted therapies in veterinary medicine. Relevant studies were identified through systematic searches PubMed, Web Science, Scopus, ScienceDirect using keywords such as “species-specific treatment strategies”, “signalling pathways”, “epigenetic paragenetic influences”, “targeted therapies”, “veterinary medicine”, “genetic variation”, “free radicals oxidative stress”. Inclusion criteria included focusing species-specific therapeutic responses, genetic influences, stress. To ensure that only most recent relevant evidence was included, peer-reviewed publications from last two decades considered. Each study selected for analysis critically appraised, with a particular emphasis methodological quality, experimental design, scientific contribution to understanding how biological influence outcomes. A special placed used comparative, cross-species approach assess variability responses potential adverse effects. The synthesises role epigenetic highlights importance understand By highlighting variation, stress, individual species differences, argues personalised approaches. emphasises an would improve care inform future research aimed at optimising therapies, ultimately leading better animal health efficacy. key is its need more protocols take into account profiles factors; it also calls greater integration studies.

Language: Английский

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Selenium-Enriched Polysaccharides from Lentinula edodes Mycelium: Biosynthesis, Chemical Characterisation, and Assessment of Antioxidant Properties

Polymers, Journal Year: 2025, Volume and Issue: 17(6), P. 719 - 719

Published: March 9, 2025

Selenium–polysaccharides possess antioxidant properties, making them promising materials for functional foods, pharmaceuticals, and clinical applications. This study examines the incorporation of selenium into polysaccharides via mycelial biosynthesis its effects on structure activity. Polysaccharides obtained from Lentinula edodes-submerged cultures grown in Se-supplemented non-supplemented media were analysed Se content (RP-HPLC/FLD), (FT-IR, HPLC, HPGPC-ELSD), activity (DPPH scavenging, reducing power, Fe2+ chelation). Two low-molecular-weight Se–heteropolysaccharides (Se-FE-1.1 Se-FE-1.2) containing ~80 125 µg/g isolated, primarily composed glucose, mannose, galactose with β-glycosidic linkages. selectively enhanced their DPPH radical scavenging assay, minimal observed iron chelation power assays. Crude Se–polysaccharides displayed highest activity, suggesting an additional contribution protein components. Our findings demonstrate that is effectively incorporated polysaccharides, altering monosaccharide composition while preserving glycosidic The selective enhancement suggests plays a specific role influencing mechanisms rather than interactions metal ions. Further research needed to clarify incorporation, nature bonding within polysaccharide molecule, impact biological

Language: Английский

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Regulation and function of inosine monophosphate dehydrogenase 2 cytoophidia during mouse and human decidualization

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111795 - 111795

Published: April 1, 2025

Language: Английский

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Optimization of Fermentation and Transcriptomic Analysis: The Impact of Aspartic Acid on the Antioxidant Activity of Termitomyces

Fermentation, Journal Year: 2025, Volume and Issue: 11(4), P. 202 - 202

Published: April 9, 2025

Termitomyces, a rare edible fungus with both nutritional and medicinal value, has garnered significant attention for its antioxidant properties. This study aims to elucidate the effects of various components on activity Termitomyces. Through assays including FRAP, DPPH, ABTS, •OH scavenging activity, strain XNQL025, which exhibits high was identified. Subsequent optimization culture medium using single-factor experiments response surface methodology revealed that aspartic acid (Asp) significantly enhances capacity this strain. Transcriptomic analysis showed Asp activates key pathways, glycolysis/gluconeogenesis, propanoate metabolism, amino sugar nucleotide valine–leucine–isoleucine biosynthesis, tryptophan along modulating peroxisome mitogen-activated protein kinase (MAPK) signaling pathways. These regulatory actions promote synthesis compounds establish multi-layered defense system comprising enzymatic (catalase) non-enzymatic (leucine/chitooligosaccharides) components. The synergistic interaction between these systems strengthens is first molecular network by thereby providing foundation development as natural antioxidant.

Language: Английский

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From Adenoma to Carcinoma: Oxidative Stress and Lipidomic Profile in Colorectal Cancer Patients

Journal of Mind and Medical Sciences, Journal Year: 2025, Volume and Issue: 12(1), P. 16 - 16

Published: April 8, 2025

Research undertaken over the past few years has brought attention to role of oxidative stress in development neoplasms by damaging nucleic acids, lipids, and proteins, thereby altering their normal function. In general, levels antioxidant enzymes are low patients with neoplasms, biomarkers used quantify have increased levels. Elevated 8-hydroxy-deoxyguanosine (8-OHdG) malondialdehyde (MDA), as well decreased enzymes, been observed diagnosed colorectal cancer (CRC) at various stages evolution, but further research is needed on correlation between these disease progression. Inflammation enhances production reactive oxygen species plays an important CRC development. Studies field metabolomics suggested that changes serum metabolites might be indicators progression from adenoma carcinoma, particularly those resulting lipid metabolism. The lipidomics pathogenesis warrants investigation, combinations (metabolic fingerprints) may potential become clinically useful markers. this article, we review our current understanding interplay stress, inflammatory markers lipidomic products CRC.

Language: Английский

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