Green-Synthesized Silver Nanoparticles Using Filipendula ulmaria (L.) Maxim. and Salvia verticillata L. Extracts Inhibit Migration and Modulate Redox Homeostasis in Human Breast Cancer Cells via Nrf-2 Signaling Pathway

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 469 - 469

Published: April 14, 2025

Breast cancer is a leading diagnosis for women around the world, with variable degree of curability. Conventional chemotherapeutic treatments often induce toxicity and damage to healthy tissues, as well development drug resistance, which why an increasing number new therapeutic regimens focus on use natural products various modifications their delivery target tissues. Silver nanoparticles possess unique physicochemical characteristics, notably increased surface area, suggesting that they hold significant potential biomedical applications. This research evaluates capacity silver green synthesized aqueous extracts Filipendula ulmaria (FUAgNPs) Salvia verticillata (SVAgNPs) alter migration redox homeostasis in human breast cell line MDA-MB-231. To determine values parameters, cells were treated five different concentrations (5, 10, 20, 50, 100 μg/mL) 24 h 72 h, while test migratory matrix metalloproteinase-9 (MMP-9) nuclear factor erythroid 2–related 2 (Nrf-2), at two (5 50 µg/mL) h. The obtained results indicate production superoxide anion radicals, malondialdehyde (MDA), nitrites after investigated treatment MDA-MB-231 cells. induced only slight elevation Nrf-2 levels, correlates weak de novo synthesis reduced glutathione (GSH), tested weaken inherent antioxidative systems was significantly reduced, MMP-9 concentration inhibited. Based demonstrated antitumor effect, confirmed by examined disrupted balance, these have additional investigation aim improving efficacy therapy. Also, FUAgNPs SVAgNPs be potentially promising novel agents against progression metastasis.

Language: Английский

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Antioxidants in cancer therapy mitigating lipid peroxidation without compromising treatment through nanotechnology

Discover Nano, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 24, 2025

Cancer treatments often exploit oxidative stress to selectively kill tumour cells by disrupting their lipid peroxidation membranes and inhibiting antioxidant enzymes. However, plays a dual role in cancer progression, acting as both promoter suppressor. Balancing through therapy remains challenge, excessive activity may compromise the efficacy of chemotherapy radiotherapy. This review explores antioxidants mitigating while maintaining treatment efficacy. It highlights recent advancements nanotechnology-based targeted delivery optimize therapeutic outcomes. A comprehensive literature was conducted using reputable databases, including PubMed, Scopus, Web Science, ScienceDirect. The search focused on publications from past five years (2020-2025), supplemented relevant studies earlier years. Keywords such "antioxidants," "lipid peroxidation," "nanotechnology therapy," "oxidative stress" were utilized. Relevant articles critically analysed, graphical illustrations created. Emerging evidence suggests that nanoparticles, liposomes, polymeric metal-organic frameworks, others, can effectively encapsulate control release minimizing systemic toxicity. Stimuli-responsive carriers with tumour-specific targeting mechanisms further enhance delivery. Studies indicate these strategies help preserve normal cells, mitigate stress-related damage, improve challenges bioavailability, stability, potential interactions standard therapies remain. Integrating nanotechnology antioxidant-based interventions presents promising approach for optimizing therapy. Future research should focus refining modulation strategies, assessing profiles during treatment, employing biomarkers determine optimal dosing. balanced use adverse effects.

Language: Английский

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ATM and p53 in aging and cancer: a double-edged sword in genomic integrity

Biogerontology, Journal Year: 2025, Volume and Issue: 26(3)

Published: May 5, 2025

Language: Английский

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Screening of oxidative stress components of cigarette smoke based on machine learning model integration

Toxicology and Applied Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 117387 - 117387

Published: May 1, 2025

Language: Английский

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Structural characterization and effect of activating autophagy and regulating oxidative stress of polysaccharide from fibrous roots of Atractylodes chinensis

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108609 - 108609

Published: May 1, 2025

Language: Английский

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Facts, Dogmas, and Unknowns About Mitochondrial Reactive Oxygen Species in Cancer

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1563 - 1563

Published: Dec. 19, 2024

Cancer metabolism is sustained both by enhanced aerobic glycolysis, characteristic of the Warburg phenotype, and oxidative metabolism. Cell survival proliferation depends on a dynamic equilibrium between mitochondrial function which heterogeneous tumors even within same tumor. During phosphorylation, electrons from NADH FADH2 originated in tricarboxylic acid cycle flow through complexes electron transport chain. Single leaks at specific chain generate reactive oxygen species (ROS). ROS are concentration-dependent double-edged sword that plays multifaceted roles cancer serve either as signaling molecules favoring cellular homeostasis or damage DNA, protein lipids, causing cell death. Several aspects biology still remain unsolved. Among unknowns actual levels become cytotoxic if toxicity it caused cumulative effect all them. In this review, we describe mechanisms production, detoxification, ROS-induced cytotoxicity, use antioxidants treatment. We also provide updated information about critical questions discuss dogmas lack adequate experimental demonstration. Overall, review brings comprehensive perspective drivers progression, inducers death, potential anticancer therapy.

Language: Английский

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Identification of genetic associations between acute myocardial infarction and non-small cell lung cancer

Frontiers in Molecular Biosciences, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 6, 2024

A growing body of evidence suggests a potential connection between myocardial infarction (MI) and lung cancer (LC). However, the underlying pathogenesis molecular mechanisms remain unclear. This research aims to identify common genes pathways MI LC through bioinformatics analysis.

Language: Английский

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Investigation of the Effects of Galectin-1 on Enzalutamide Sensitivity and Cellular Functions in Prostate Cancer Cells and Its Clinical Diagnostic Value

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Prostate cancer (PCa) is the most common malignancy in men worldwide, with an increasing incidence rate, and many patients are diagnosed at advanced stage metastasis, where bone metastasis frequent. Enzalutamide (ENZ) first-line treatment for castration-resistant prostate (CRPC) has shown efficacy prolonging survival; however, some develop drug resistance. Additionally, benign prostatic hyperplasia (BPH) a condition aging men, symptoms similar to PCa, highlighting need improved diagnostic therapeutic approaches. In present study, we integrated public databases, RNA sequencing (RNA-Seq), bioinformatics tools, utilizing CRISPR-Cas9 technology knock out galectin-1 (LGALS1) PCa cells comprehensively investigate its impact on ENZ sensitivity, cellular functions, underlying mechanisms. Clinical tissue samples were also analyzed assess clinical significance of LGALS family members. Our findings indicated that sensitivity depends AR expression, LGALS1 knockout AR-expressing enhances sensitivity. RNA-Seq revealed suppresses energy metabolism disrupts oxidative stress balance. high-expression reduced proliferation, altered cell cycle, decreased migration adhesion. Clinically, both overexpressed bone-metastatic suggesting their potential as targets biomarkers.

Language: Английский

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4-Pyridone-3-carboxamide-1-β-D-ribonucleoside Reduces Cyclophosphamide Effects and Induces Endothelial Inflammation in Murine Breast Cancer Model

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 35 - 35

Published: Dec. 24, 2024

4-pyridone-3-carboxamide-1-β-D-ribonucleoside (4PYR) is a nicotinamide derivative, considered new oncometabolite. 4PYR formation induced cytotoxic effect on the endothelium. Elevated blood concentration was observed in patients with cancer. Still, little known about metabolic and functional effects of this pathology. The study aimed to investigate whether toxic accumulation may affect activity anticancer therapy cyclophosphamide orthotropic model breast Female Balb/c mice were injected 4T1 cancer cells assigned into three groups: treated PBS (Control), cyclophosphamide-treated (+CP), 4PYR-treated (+4PYR), both CP(+4PYR+CP) for 28 days. Afterward, serum samples, liver, muscle, spleen, heart, lungs, aortas, tumor tissue collected analysis concentrations nucleotides, metabolites, its as well liver level cytochrome P450 enzymes. treatment caused elevation 4PYR, monophosphate adenine dinucleotide (NAD+) analog-4PYRAD. Blood 4PYRAD +4PYR+CP reduced comparison +4PYR. Tumor growth final mass significantly decreased +CP did not differ +4PYR Control. However, we substantial increase these parameters compared +CP. extracellular adenosine deamination rate measured assess vascular inflammation, it higher than Treatment CP highest ATP hydrolysis rate. administration significant CYP2C9 reduction CYP3A4 Control In additional experiments, healthy without cancer, (4PYR w/o cancer) (Control cancer), where an proinflammatory cytokine expression interferes induces pro-inflammatory shift endothelial enzymes, probably by affecting metabolism This observation have crucial implications various drugs metabolized P450.

Language: Английский

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Synergistic Effects of Omega-3 Fatty Acids and Physical Activity on Oxidative Stress Markers and Antioxidant Mechanisms in Aged Rats

Nutrients, Journal Year: 2024, Volume and Issue: 17(1), P. 96 - 96

Published: Dec. 29, 2024

Background: Aging induces degenerative processes in the body, contributing to onset of various age-associated diseases that affect population. Inadequate dietary habits and low physical activity are major contributors increased morbidity during aging. This study aimed investigate combined effects omega-3 fatty acid supplementation on markers oxidative stress antioxidant defense mechanisms aged male Wistar rats (23–24 months). Methods: The were randomly divided into four experimental groups: a sedentary control (placebo, no exercise), trained (placebo moderate-intensity graded aerobic exercise; Ex), two groups supplemented with (160 mg/kg body weight; O1 + Ex) high (320 O2 doses acids. biochemical functional parameters related sarcopenia measured blood gastrocnemius muscle. Results: results demonstrated dose-dependent, synergistic activity. higher dose weight) improved plasma capacity (TEAC, +21.01%, p < 0.01) GPx (+78.05%, 0.05) while reducing CAT erythrocytes (−19.92%, 0.05), likely as an adaptive response. Combined interventions also normalized cholesterol levels, (stride length, +14.82%, 0.001), enhanced protection rats. Conclusions: These findings highlight potential combining counteract aging-related changes. Further research is needed elucidate underlying evaluate long-term benefits these strategies aging populations.

Language: Английский

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