Metabolomics insights into the protective molecular mechanism of Vaccinium myrtillus against oxidative stress in intestinal cells DOI Creative Commons

Sara Novi,

Vicky Caponigro, Maria Rosaria Miranda

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 13, 2025

Abstract Blueberry ( Vaccinium myrtillus L. ) is a rich source of secondary metabolites known for their potent antioxidant, anti-inflammatory, and cytoprotective properties. These compounds are essential in neutralizing reactive oxygen species (ROS), which implicated oxidative stress-related diseases. In this study, we induced stress IEC-6 small intestine cells using hydrogen peroxide (H 2 O ), creating cellular model to investigate the biochemical response. The obtained results showed that blueberry extract (BLUBE) significantly exhibited strong antioxidant capacity, as evidenced by DPPH, FRAP ABTS vitro tests. Additionally, BLUBE effectively inhibited release enhanced response, indicated improved wound healing clonogenic potential reduction fibers rearrangement apoptosis. Metabolomic analysis, specifically High-Resolution Mass Spectrometry (HR-MS), was employed elucidate metabolic alterations associated with protective activity against cells. Chemometric approaches were applied preprocess data, explore variability, identify systematic biases, ensuring removal batch effects other experimental artifacts. A Partial Least Squares Discriminant Analysis classification confirmed clear group stratifications high accuracy (98.75 ± 2.31%), sensitivity, specificity, aiding identification significant pathway enrichment analysis. Key pathways, including sphingolipid metabolism, taurine hypotaurine glycerophospholipid cysteine methionine modulated, supporting basis BLUBE’s effects. fact, able partially reverse downregulation these reducing promoting cell survival. This study highlights power HR-MS-based metabolomics uncovering mechanisms nutraceuticals emphasizes agent It also underscores growing significance food pharmaceutical industries.

Language: Английский

Metabolomics insights into the protective molecular mechanism of Vaccinium myrtillus against oxidative stress in intestinal cells DOI Creative Commons

Sara Novi,

Vicky Caponigro, Maria Rosaria Miranda

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 13, 2025

Abstract Blueberry ( Vaccinium myrtillus L. ) is a rich source of secondary metabolites known for their potent antioxidant, anti-inflammatory, and cytoprotective properties. These compounds are essential in neutralizing reactive oxygen species (ROS), which implicated oxidative stress-related diseases. In this study, we induced stress IEC-6 small intestine cells using hydrogen peroxide (H 2 O ), creating cellular model to investigate the biochemical response. The obtained results showed that blueberry extract (BLUBE) significantly exhibited strong antioxidant capacity, as evidenced by DPPH, FRAP ABTS vitro tests. Additionally, BLUBE effectively inhibited release enhanced response, indicated improved wound healing clonogenic potential reduction fibers rearrangement apoptosis. Metabolomic analysis, specifically High-Resolution Mass Spectrometry (HR-MS), was employed elucidate metabolic alterations associated with protective activity against cells. Chemometric approaches were applied preprocess data, explore variability, identify systematic biases, ensuring removal batch effects other experimental artifacts. A Partial Least Squares Discriminant Analysis classification confirmed clear group stratifications high accuracy (98.75 ± 2.31%), sensitivity, specificity, aiding identification significant pathway enrichment analysis. Key pathways, including sphingolipid metabolism, taurine hypotaurine glycerophospholipid cysteine methionine modulated, supporting basis BLUBE’s effects. fact, able partially reverse downregulation these reducing promoting cell survival. This study highlights power HR-MS-based metabolomics uncovering mechanisms nutraceuticals emphasizes agent It also underscores growing significance food pharmaceutical industries.

Language: Английский

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