Enhanced Antioxidant and Protective Effects of Fermented Solanum melongena L. Peel Extracts Against Ultraviolet B-Induced Skin Damage

Nutrients, Journal Year: 2025, Volume and Issue: 17(5), P. 847 - 847

Published: Feb. 28, 2025

Background/Objectives: The skin, being the body's outermost organ, plays a vital role in protecting against various external stimuli. Ultraviolet generates reactive oxygen species (ROS), promoting secretion of matrix metalloproteinases (MMPs) and inducing collagen degradation. Many studies have been conducted to identify natural substances that can prevent or delay harmful effects UV. Methods: A wound healing assay, DCF-DA (ROS) JC-1 assay were performed assess bio-converted eggplant peels (BEPs) on human dermal fibroblasts (HDFs). Western blot analysis was also understand underlying mechanisms for their effects. Finally, hematoxylin-eosin staining immunohistochemistry animal studies. Results: Our study evaluated antioxidant efficacy BEPs fermented with Lactobacillus plantarum hydrogen peroxide (H2O2)-HDFs UVB-induced skin damage hairless mice. We demonstrated exhibited enhanced properties compared non-fermented (EPs). facilitated H2O2-damaged HDFs, reduced ROS levels, restored mitochondrial membrane potential. suppressed phosphorylation ERK, p38, JNK as mechanism. further dietary supplementation downregulated metalloproteinase 1 (MMP1) expression upregulated I (COL1) UVB-damaged mice, indicating more effective EPs. Conclusions: suggest hold significant potential protective agent mitigating health.

Language: Английский

Pinitol Improves Lipopolysaccharide-Induced Cellular Damage in Human Dermal Microvascular Endothelial Cells

Molecules, Journal Year: 2025, Volume and Issue: 30(7), P. 1513 - 1513

Published: March 28, 2025

3-O-Methyl-D-chiro-inositol (pinitol) has been reported to possess insulin-like effects and is known as one of the anti-diabetic agents for improving muscle liver function. However, beneficial pinitol on human dermal microvascular endothelial cells (HDMECs) are not well understood. In this study, we investigated whether could protect HDMECs from damage induced by lipopolysaccharides (LPSs), which cause various cell defects. We observed that enhanced wound healing LPS-damaged HDMECs. found significantly downregulated LPS-induced upregulation reactive oxygen species (ROS). Pinitol also restored mitochondrial membrane potential in these cells. Immunofluorescence analysis revealed notably reduced nuclear localization NF-κB Furthermore, demonstrated decreased phosphorylation levels MAPK family Interestingly, improved tube formation Taken together, suggest exerts several may be a promising therapeutic agent vascular-related skin diseases.

Language: Английский