Antioxidant Mechanisms of the Protective Action of Selenase in Experimental Chronic Generalized Periodontitis

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(3), P. 186 - 186

Published: March 12, 2025

Inflammatory periodontal diseases, despite all the efforts of modern dentistry, remain an important predictor tooth loss worldwide. Oxidative stress plays a crucial role in pathogenesis periodontitis, making use antioxidants attractive option for its treatment. Our attention was drawn to selenium compound Selenase as antioxidant therapeutic agent. In this study, we modeled calcium-deficient prooxidant chronic generalized periodontitis (CGP) model white non-linear rats. Then, after 14 days, (50 μg/kg) and Mexidol (250 mg/kg) were administered intragastrically. Blood samples from animals analyzed using ELISA biochemical methods determine Cu-Zn SOD, nitrotyrosine, GPX-4, iNOS, NOx, GSH, GSSG levels. The CGP led typical clinical signs including hyperemia, edema, gingival pocket formation, bleeding, mobility, well increase molecular–biochemical markers nitrosative reduction endogenous blood. resulted decrease manifestations CGP, reduced NOx levels, SOD GPX-4 compared control group (p < 0.05). had less pronounced effect on these

Language: Английский

HSP70 Modulators for the Correction of Cognitive, Mnemonic, and Behavioral Disorders After Prenatal Hypoxia

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 982 - 982

Published: April 17, 2025

Background/Objectives: Prenatal hypoxia (PH) is a leading cause of nervous system disorders in early childhood and subsequently leads to decline the cognitive mnemonic functions central (such as memory impairment, reduced learning ability, information processing). It also increases anxiety risk brain adulthood. Compensatory–adaptive mechanisms mother–placenta–fetus system, which enhance fetus’s CNS resilience, are known, including activation endogenous neuroprotection response hypoxic injury through pharmacological modulation HSP70. Methods: To evaluate effect HSP70 modulators—Cerebrocurin, Angiolin, Tamoxifen, Glutaredoxin, Thiotriazoline, HSF-1 (heat shock factor 1 protein), well Mildronate Mexidol—on motor skills, exploratory behaviors, psycho-emotional activities, learning, memories offspring after PH. Experimental PH was induced by daily intraperitoneal injections sodium nitrite solution into pregnant female rats from 16th 21st day pregnancy at dose 50 mg/kg. The newborns received Angiolin (50 mg/kg), Thiotriazoline Mexidol (100 Cerebrocurin (150 µL/kg), L-arginine (200 Glutaredoxin µg/kg), or mg/kg) for 30 days. At month, were tested open field test, 2 months, they trained working spatial radial maze. Results: Modeling led persistent impairments activity, behavior, decrease cognitive–mnestic CNS. found that had most pronounced effects on indicators activity status 1-month-old animals They exhibited significant cognitive-enhancing memory-supporting during training evaluation skill retention maze 2-month-old Conclusions: first time, we obtained experimental data modulators following intrauterine hypoxia. Based results this study, identified agents promising neuroprotective perinatal

Language: Английский

Similar normalizing effect of HSP70 and YB-1 stress proteins on the brain transcription of a mouse model of Alzheimer’s disease

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Previously, we demonstrated therapeutic effects of intranasal administration human HSP70 and YB-1 proteins in various models AD including olfactory bulbectomized (OBX) mice. Herein, investigated the effect these two stress on transcription hippocampus cortex OBX In spite different structure both frequently caused pronounced normalizing changes same genes. Thus, genes that normalized their expression due to action recHSP70 or recYB-1 coincide by 61%. More than 60% common target exhibited complete restoration a pattern control sham-operated Notably, number with altered after was twice as high cortex. brain mice application led normalization many related pathogenesis participated metabolism Aβ tau proteins. Importantly, also include loci involved ribosome biogenesis, well responsible for neurotransmitter transport, cognition, apoptosis, mitochondria functions protection from oxidative stress. A significant positive all six cell types studied most observed astrocytes. Besides AD-related genes, several implicated development major depression other neurodegenerative diseases.

Language: Английский