The Protective Effects of Lactobacillus reuteri Combined With Clostridium butyricum Miyairi 588 on Intestinal Barrier Function, Water Transport, and Oxidative Stress in a Rat Model of 5FU‐Induced Diarrhea DOI Creative Commons
Sheau‐Chung Tang,

Wen‐Chen Chuang,

Jiuan‐Miaw Liao

et al.

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(6)

Published: May 23, 2025

ABSTRACT 5‐Fluorouracil (5FU) is a commonly employed and highly effective chemotherapeutic agent in clinical oncology. Nevertheless, one of the most frequent debilitating adverse effects associated with 5FU treatment diarrhea. These gastrointestinal complications can affect patients' quality life adherence to regimens. Consequently, addressing mitigating diarrhea during therapy presents critical urgent challenge oncological care. This study investigated whether probiotic Lactobacillus reuteri combined Clostridium butyricum Miyairi 588 (LCs) alleviate 5FU‐induced potential mechanism. Wistar rats received (50 mg/kg, intraperitoneal injection) for 5 consecutive days establish colitis model. LCs were administered 15 before injection continued until day sacrifice. Tissue morphology, inflammatory oxidative stress markers, as well expression mRNA related intestinal barrier integrity, apoptosis, aquaporins (AQPs) evaluated colon tissue samples. analyses used hematoxylin eosin staining, enzyme‐linked immunosorbent assay (ELISA), quantitative real‐time reverse transcription‐polymerase chain reaction (qRT‐PCR) techniques. Additionally, concentrations short‐chain fatty acids (SCFAs) measured using gas chromatography‐flame ionization detection (GC‐FID) analysis. In this model, mitigated weight loss, diarrhea, bloody stool, shortened length, colonic histopathology. Treatment resulted reduced levels MDA, TNF‐α, IL‐1β, MPO activity, decreased IFN‐γ, AKT, NF‐κB, iNOS. significantly downregulated VCAM‐1, CXCL4, MAPK, caspase‐3, while upregulating tight junction protein occludin expression. also notably diminished AQP7, VIP, PKA. demonstrates that have therapeutic on colitis, primarily through their antioxidant properties, anti‐apoptotic effects, mucosal integrity maintenance, neutrophil infiltration reduction, cytokines aquaporin modulation.

Language: Английский

Role of Antioxidants in Modulating the Microbiota–Gut–Brain Axis and Their Impact on Neurodegenerative Diseases DOI Open Access
Natalia Kurhaluk, Piotr Kamiński, Rafał Bilski

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3658 - 3658

Published: April 12, 2025

This narrative review presents the role of antioxidants in regulating gut microbiota and impact on gut–brain axis, with a particular focus neurodegenerative diseases, such as Alzheimer’s (AD) Parkinson’s disease (PD). These diseases are characterised by cognitive decline, motor dysfunction, neuroinflammation, all which significantly exacerbated oxidative stress. elucidates contribution damage to progression explores potential mitigate these pathological processes through modulation associated pathways. Based recent studies retrieved from reputable databases, including PubMed, Web Science, Scopus, this article outlines mechanisms influence health exert neuroprotective effects. Specifically, it discusses how antioxidants, polyphenols, vitamins, flavonoids, contribute reduction reactive oxygen species (ROS) production thereby promoting neuronal survival minimising brain. In addition, modulating key molecular pathways involved stress NF-κB, Nrf2, MAPK, PI3K/AKT pathways, regulate ROS generation, inflammatory cytokine expression, antioxidant responses essential for maintaining cellular homeostasis both central nervous system. complex relationship between gut-derived metabolites, stress, highlighting dysbiosis—an imbalance microbiota—can exacerbate accelerating AD PD. The also examines short-chain fatty acids (SCFAs) produced beneficial bacteria attenuate neuroinflammation damage. Furthermore, therapeutic microbiota-targeted interventions, delivery probiotics prebiotics, innovative strategies restore microbial support brain health. By synthesising current knowledge interplay underlying neurodegeneration, highlights promise antioxidant-based interventions mitigating progression. It need further research into antioxidant-rich dietary microbiota-focused therapies promising avenues prevention treatment diseases.

Language: Английский

Citations

0
The Protective Effects of Lactobacillus reuteri Combined With Clostridium butyricum Miyairi 588 on Intestinal Barrier Function, Water Transport, and Oxidative Stress in a Rat Model of 5FU‐Induced Diarrhea DOI Creative Commons
Sheau‐Chung Tang,

Wen‐Chen Chuang,

Jiuan‐Miaw Liao

et al.

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(6)

Published: May 23, 2025

ABSTRACT 5‐Fluorouracil (5FU) is a commonly employed and highly effective chemotherapeutic agent in clinical oncology. Nevertheless, one of the most frequent debilitating adverse effects associated with 5FU treatment diarrhea. These gastrointestinal complications can affect patients' quality life adherence to regimens. Consequently, addressing mitigating diarrhea during therapy presents critical urgent challenge oncological care. This study investigated whether probiotic Lactobacillus reuteri combined Clostridium butyricum Miyairi 588 (LCs) alleviate 5FU‐induced potential mechanism. Wistar rats received (50 mg/kg, intraperitoneal injection) for 5 consecutive days establish colitis model. LCs were administered 15 before injection continued until day sacrifice. Tissue morphology, inflammatory oxidative stress markers, as well expression mRNA related intestinal barrier integrity, apoptosis, aquaporins (AQPs) evaluated colon tissue samples. analyses used hematoxylin eosin staining, enzyme‐linked immunosorbent assay (ELISA), quantitative real‐time reverse transcription‐polymerase chain reaction (qRT‐PCR) techniques. Additionally, concentrations short‐chain fatty acids (SCFAs) measured using gas chromatography‐flame ionization detection (GC‐FID) analysis. In this model, mitigated weight loss, diarrhea, bloody stool, shortened length, colonic histopathology. Treatment resulted reduced levels MDA, TNF‐α, IL‐1β, MPO activity, decreased IFN‐γ, AKT, NF‐κB, iNOS. significantly downregulated VCAM‐1, CXCL4, MAPK, caspase‐3, while upregulating tight junction protein occludin expression. also notably diminished AQP7, VIP, PKA. demonstrates that have therapeutic on colitis, primarily through their antioxidant properties, anti‐apoptotic effects, mucosal integrity maintenance, neutrophil infiltration reduction, cytokines aquaporin modulation.

Language: Английский

Citations

0