Optimization of Extraction Process, Structural Characterization, and Antioxidant and Hypoglycemic Activity Evaluation of Polysaccharides From the Medicinal and Edible Plant: Cistanche deserticola Ma

Phytochemical Analysis, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

ABSTRACT Introduction Cistanche deserticola Ma (CD), an edible and medicinal plant native to Xinjiang, Inner Mongolia, Gansu in China, is rich bioactive polysaccharides known for their health‐promoting properties. The of C. (CDPs) have been shown possess a range beneficial activities, including immunomodulatory, anti‐aging, antioxidant, anti‐osteoporosis effects. Objective This study seeks identify the optimal conditions extracting CDPs using hot water. Additionally, it aims evaluate chemical properties, antioxidant activity, hypoglycemic effects, cytotoxicity. findings will provide theoretical foundation potential use functional foods pharmaceuticals. Methodology employed response surface methodology optimize water extraction CDPs. extracted were characterized chemical, spectroscopic, instrumental analyses. Furthermore, cytotoxicity evaluated through relevant assays assess health benefits. Results Under conditions, yield was 45.85% ± 1.91%. identified as acidic heteropolysaccharides with wide molecular weight distribution, ranging from 0.3 128.2 kDa. They composed primarily glucose (51.21%), arabinose (32.86%), galactose (17.88%), smaller amounts galacturonic acid (4.66%), rhamnose (1.85%), mannose (1.32%), glucosamine hydrochloride (1.08%), xylose (0.56%). Antioxidant demonstrated that exhibited significant free radical scavenging metal ion chelation, reducing power. inhibited α‐glucosidase α‐amylase vitro mixed‐type mechanism, well static fluorescence quenching. Cytotoxicity showed nontoxic L02 AML12 cells. Conclusion offers pharmaceuticals provides valuable insights development new agents natural sources.

Language: Английский

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Plant Sources Responsible for the Chemical Composition and Main Bioactive Properties of Poplar-Type Propolis

Plants, Journal Year: 2020, Volume and Issue: 10(1), P. 22 - 22

Published: Dec. 24, 2020

Propolis is a resinous mixture, made by the honeybees from substances collected tree or other plant buds, exudates, resins found in stem, branches, leaves of different plants. The geographical origin propolis given sources respective areas. Different studies have classified this bee product according to vegetal material same Poplar-type has widest spread world, temperate zones Europe, Asia, North America. name main source where bees are collecting resins, although present mentioned Pinus spp., Prunus Acacia spp. and also Betula pendula, Aesculus hippocastanum, Salix alba important for “poplar-type” propolis. aim review identify material’s chemical composition activities balms used produce poplar-type compare it with final similar regions. relevance find similarities between properties latest determination methods bioactive compounds plants reviewed.

Language: Английский

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Active packaging coating based on Lepidium sativum seed mucilage and propolis extract: Preparation, characterization, application and modeling the preservation of buffalo meat

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(10), P. e0311802 - e0311802

Published: Oct. 9, 2024

Buffalo meat is naturally perishable, making it susceptible to spoilage due its high moisture content and vulnerability microbial contamination. Edible coatings have attracted attention as a packaging method that can prolong the shelf life of meat. The study aimed examine impact combination Lepidium sativum mucilage (LS) coating propolis extract (PE) on prolonging buffalo chemical characteristics (chemical compounds, total phenol (TPC), flavonoid (TFC), antioxidant activity, cytotoxicity) antimicrobial activity PE (disk diffusion agar, well minimum inhibitory concentration, bactericidal concentration) were investigated. effect cell wall pathogenic bacteria was examined using scanning electron microscope. Biological properties LS (TPC, TFC, (pour plate method)) Different concentrations (0, 0.5, 1.5, 2.5%) added mixture containing LS, their effects extending samples stored at 4°C for 9 days assessed. included gallic acid, benzoic syringic 4-3 dimethoxy cinnamic p-coumaric myricetin, caffeic luteolin, chlorogenic apigenin. determined TPC 36.67 ± 0.57 mg GAE/g TFC 48.02 0.65 QE/g. extract's radical scavenging ranged from 0 76.22% DPPH radicals 50.31% ABTS radicals. viability C115 HeLa observed be 94.14 μg/mL. exhibited strong against bacteria. 15.23 0.43 11.51± 0.61 429.65 1.28 μg/mL 403.59 1.46 microbiological analysis revealed LS+2.5%PE treatment most effective in inhibiting growth viable count (6.23 vs. 8.00 log CFU/g), psychrotrophic (3.71 4.73 coliforms (2.78 3.70 fungi (2.39 3.93 CFU/g) compared control sample. addition edible also demonstrated concentration-dependent preserving moisture, pH, color, hardness Sensory evaluation results suggested incorporating into extended by three days. In second stage this paper, investigation employed two distinct forecasting methodologies: Radial Basis Function (RBF) Support Vector Machine (SVM), predict range quality indicators coated products. Upon comparison, RBF model higher level accuracy, showcasing exceptional capacity closely match experimental outcomes. Therefore, type food coating, renowned properties, has potential effectively package preserve perishable delicate items, such

Language: Английский

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Protective Effect of Honey and Propolis against Gentamicin‐Induced Oxidative Stress and Hepatorenal Damages

Oxidative Medicine and Cellular Longevity, Journal Year: 2021, Volume and Issue: 2021(1)

Published: Jan. 1, 2021

Bee products are a promising source of phenolic compounds with strong antioxidant activity. The present study was designed to explore the protective effect honey, propolis, and their combination on gentamicin-induced oxidative stress hepatorenal dysfunction. This conducted male Wistar rats by intraperitoneal injections gentamicin (120 mg/kg BW/day, i.p.) or normal saline (1 ml/kg for 10 consecutive days. Honey (2 g/kg BW), propolis (100 were given daily gavage groups. samples evaluated phytochemical composition capacity. in vitro investigations showed that especially extract have high power associated presence several such as flavonoids, flavan-3-ols, hydroxybenzoic acids, hydroxycinnamic stilbenes, while honey contains only acids acids. It also shown simultaneous treatment alone association prevented changes caused administration improved hepatic renal functions. Changes administration, observed vivo experiments, include significant elevation uric acid, urea, creatinine, enzyme levels (ALT, AST, ALP) kidney biochemical (an increase creatinine decrease albumin total protein) well remarkable liver markers (CAT, GPx, GSH) MDA levels. Overall, it can be concluded might useful management diseases induced xenobiotics.

Language: Английский

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Simultaneous Optimization of Extraction Yield, Phenolic Compounds and Antioxidant Activity of Moroccan Propolis Extracts: Improvement of Ultrasound-Assisted Technique Using Response Surface Methodology

Processes, Journal Year: 2022, Volume and Issue: 10(2), P. 297 - 297

Published: Feb. 2, 2022

Propolis has given rise to refreshing interest in recent years the field of conventional medicine. Its extraction represents an important process that requires optimal conditions, which strongly affect yield extraction, total polyphenols, flavonoid content, and radical scavenging capacity markers. The objective present study was optimize ultrasound-assisted conditions Moroccan propolis. studied responses were yield, contents (TPC, TFC), antioxidant activity extract evaluated by DPPH-IC50 FRAP-EC50 assays. response surface methodology (RSM) specifically Box–Behnken design (BBD) used, taking into account three variables: sonication time (min), solvent/propolis ratio (mL/g), ethanol concentration (%). After realization experiments data analysis, values 15.39%, 192 mg GAE/g propolis,45.15 QEq/g, 29.8 µg/mL, 128.3 µmol Fe2+/g for TPC, TFC, DPPH-IC50, FRAP-EC50, respectively. Besides, ultrasound 15 min time, 30 mL/g ratio, 40% concentration. All obtained experimental good agreement with predicted values, suggesting using optimization prudently chosen.

Language: Английский

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Egyptian propolis extract for functionalization of cellulose nanofiber/poly(vinyl alcohol) porous hydrogel along with characterization and biological applications

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: May 12, 2023

Bee propolis is one of the most common natural extracts and has gained significant interest in biomedicine due to its high content phenolic acids flavonoids, which are responsible for antioxidant activity products. The present study report that extract (PE) was produced by ethanol surrounding environment. obtained PE added at different concentrations cellulose nanofiber (CNF)/poly(vinyl alcohol) (PVA), subjected freezing thawing freeze drying methods develop porous bioactive matrices. Scanning electron microscope (SEM) observations displayed prepared samples had an interconnected structure with pore sizes range 10-100 μm. performance liquid chromatography (HPLC) results showed around 18 polyphenol compounds, highest amounts hesperetin (183.7 µg/mL), chlorogenic acid (96.9 µg/mL) caffeic (90.2 µg/mL). antibacterial indicated both PE-functionalized hydrogels exhibited a potential antimicrobial effects against Escherichia coli, Salmonella typhimurium, Streptococcus mutans, Candida albicans. vitro test cell culture experiments cells on greatest viability, adhesion, spreading cells. Altogether, these data highlight interesting effect bio-functionalization enhance biological features CNF/PVA hydrogel as functional matrix biomedical applications.

Language: Английский

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A melissopalynological and chemical characterization of Anatolian propolis and an assessment of its antioxidant potential

European Food Research and Technology, Journal Year: 2023, Volume and Issue: 249(5), P. 1213 - 1233

Published: Feb. 23, 2023

Language: Английский

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Discrimination of Green Coffee (Coffea arabica and Coffea canephora) of Different Geographical Origin Based on Antioxidant Activity, High-Throughput Metabolomics, and DNA RFLP Fingerprinting

Antioxidants, Journal Year: 2023, Volume and Issue: 12(5), P. 1135 - 1135

Published: May 21, 2023

The genus Coffea is known for the two species C. arabica (CA) and canephora (CC), which are used to prepare beverage coffee. Proper identification of green beans coffee varieties based on phenotypic phytochemical/molecular characteristics. In this work, a combination chemical (UV/Vis, HPLC-DAD-MS/MS, GC-MS, GC-FID) molecular (PCR-RFLP) fingerprinting was discriminate commercial accessions from different geographical origin. highest content polyphenols flavonoids always found in CC accessions, whereas CA showed lower values. ABTS FRAP assays significant correlation between phenolic antioxidant activity most accessions. We identified 32 compounds, including 28 four N-containing compounds. contents caffeine melatonin were detected levels quercetin kaempferol derivatives Fatty acids characterized by low linoleic cis octadecenoic acid high amounts elaidic myristic acid. Discrimination according their origin achieved using high-throughput data analysis, combining all measured parameters. Lastly, PCR-RFLP analysis instrumental recognition markers majority Using restriction enzyme AluI trnL-trnF region, we clearly discriminated arabica, cleavage performed enzymes MseI XholI 5S-rRNA-NTS region produced specific discrimination patterns useful correct This work extends our previous studies provides new information complete flavonoid profile, with DNA assess

Language: Английский

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Preliminary Insights into the Cyto/Genoprotective Properties of Propolis and Its Constituent Galangin In Vitro

Toxics, Journal Year: 2025, Volume and Issue: 13(3), P. 194 - 194

Published: March 8, 2025

Propolis has been well known for centuries as a natural preventive and therapeutic agent. Its numerous health benefits are mainly attributed to its high content of phenolic compounds that have remarkable antioxidant activity. Since phenolics may exert dual nature (pro-oxidant antioxidant) the aim this study was investigate safety profile ethanolic extract propolis related flavonoid galangin their ability protect lymphocytes from irinotecan-induced cyto/genotoxicity in vitro. Isolated human peripheral blood were exposed 3 h three concentrations corresponding average daily dose 0.25 mL [propolis 70% ethanol (3:7, w/w)], five- ten-fold higher concentration. Cyto- genoprotective effects tested using cytokinesis-block micronucleus cytome assay. Treatment with selected exerted biocompatibility diminished level cytogenetic damage caused by irinotecan. at same concentration offered stronger protective effect than single galangin. Also, apoptosis prevailing mechanism cell death our experimental conditions. These preliminary results speak favour future investigations other available methods models.

Language: Английский

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Red Propolis and Its Dyslipidemic Regulator Formononetin: Evaluation of Antioxidant Activity and Gastroprotective Effects in Rat Model of Gastric Ulcer

Nutrients, Journal Year: 2020, Volume and Issue: 12(10), P. 2951 - 2951

Published: Sept. 26, 2020

Propolis has various pharmacological properties of clinical interest, and is also considered a functional food. In particular, hydroalcoholic extracts red propolis (HERP), together with its isoflavonoid formononetin, have recognized antioxidant anti-inflammatory properties, known added value against dyslipidemia. this study, we report the gastroprotective effects HERP (50–500 mg/kg, p.o.) formononetin (10 in ethanol non-steroidal drug-induced models rat ulcer. The volume, pH, total acidity were evaluated gastric secretion parameters using pylorus ligature model, assessment mucus contents. anti-Helicobacter pylori activities agar-well diffusion method. our experiments, (250 500 mg/kg) reduced (p < 0.001) lesion areas ethanol-induced ulcer 0.05) indices indomethacin-induced model. Administration to significantly decreased 0.01) volumes increased production. We shown HERP. obtained results indicate that are acute models, suggesting prominent role

Language: Английский

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