Scientia Horticulturae,
Journal Year:
2023,
Volume and Issue:
322, P. 112388 - 112388
Published: Aug. 8, 2023
Ginsenosides,
as
the
main
pharmacological
activity
ingredients
of
ginseng,
can
be
used
to
treat
various
diseases.
Different
cultivars
ginseng
have
been
developed
through
domestication
and
artificial
selection.
However,
it
is
still
unclear
whether
there
are
differences
in
content
ginsenosides
regulatory
mechanisms
involved
among
cultivars.
In
this
study,
roots,
stems,
leaves
different
were
analyzed
for
transcriptome
data.
The
results
showed
that
total
was
higher
than
roots
moreover,
numerous
monomers
varied
significantly
Among
105
synthesis
genes,
most
UDP-glycosyltransferases
(UGTs)
highly
expressed
while
genes
from
2-C-methyl-d-erythritol-4-phosphate
(MEP)
pathway
overexpressed
leaves.
A
large
number
differentially
alternative
splicing
(DASGs)
found
comparison
groups,
overlapping
DEGs
DASGs
rare,
furthermore,
functions
various,
suggesting
(AS)
differential
expression
might
play
roles.
series
AS
events
Weighted
Gene
Co-Expression
Network
Analysis
(WGCNA)
constructed
analyze
gene-ginsenosides
association
gene
network.
Then,
extensive
transcription
factors
(TFs)
identified
correlated
modules,
including
AP2/ERF,
WRKY,
MYB,
bHLH
et
al.,
levels
these
TFs
with
genes.
Therefore,
influenced
structure
TFs,
respectively,
thereby
modulating
accumulation
ginsenosides.
provided
a
theoretical
basis
investigating
mechanism
ginsenoside
offered
new
perspective
development
natural
medicines.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Nov. 25, 2024
This
study
end
to
develop
nanoemulsions
of
Panax
ginseng
dry
extract
and
evaluate
the
potential
impact
these
versus
free
Vit.E
in
recovering
male
infertility
induced
rats.
Nanoemulsions
were
prepared
by
oil
water
method.
The
designed
samples
characterized
TEM,
zeta
sizer,
FTIR,
TGA.
vitro
included
DPPH
assay
estimate
radical
scavenging
activity
suggested
treatments.
vivo
100
adult
Wistar
rats
which
assigned
into
10
equal
groups;
five
groups
young
weighting
(150-200
g)
aged
(350-400
g).
Group
I,
negative
control.
II,
bisphenol-A
(BPA).
III,
BPA+
nanoemulsion.
IV,
extract.
V,
BPA
+Vit.E.
After
40
days,
serum
total
testosterone,
MDA,
8-OHdG
AGEs
estimated.
Besides,
histological
investigation
testicular
tissue
sections
was
performed.
TEM
imaging
indicated
spherical
shape
with
diameter
range
from
2
50
nm,
size
distribution
62
123
d.nm.
-32.8
-38.9
mV.
FTIR
spectra
revealed
common
active
nanoemulsions.
thermal
stability
up
207
ºC.
results
showed
%
inhibition
for
49.38%
(for
young-treated
group
Sample
A)
72.28%
aged-treated
B),
while
30.27%
C)
56.76%
D),
32.36%
E)
36.39%
F).Thus
exhibit
radicals
more
than
Vit.E.
findings
elucidated
that
successfully
revers
progressive
insult
on
fertility
significantly
enhance
testosterone
(2.87±0.318)
(1.63±0.033)
levels,
decrease
MDA
(2.77±0.018),
(6.76±0.174)
(92.60±1.701)
levels.
Interestingly,
most
promising
outcomes
recorded
upon
treatment
In
conclusion
developed
nanoemulsion
could
be
used
as
a
strategy
improving
defects
rescuing
sex
hormones,
neutralizing
oxidative
stress
retrieving
structural
organization
testes.
Applied Sciences,
Journal Year:
2023,
Volume and Issue:
13(24), P. 13171 - 13171
Published: Dec. 12, 2023
The
family
Bignoniaceae
includes
Handroanthus
impetiginosus
trees,
which
are
sparsely
distributed
in
the
northeast
of
Brazil.
Natural
products
play
a
vital
role
discovery
drugs
for
various
diseases.
Many
plants
have
been
used
as
sources
medicines
because
their
chemical
diversity
and
potent
bioactivity.
has
traditionally
to
cure
wide
range
illnesses,
such
cancer,
oxidative
stress,
inflammation.
This
work
highlights
cytotoxicity,
cell
death,
routes
apoptosis
lung
cancer
cells
(A549)
anti-inflammatory
antioxidant
effects
roasted
(lapacho/taheebo)
normal
cells.
viability
assay
indicated
that
puffing
taheebo
is
nontoxic
line
up
500
µg/mL
but
significantly
toxic
A549
lapacho/taheebo
also
increases
reactive
oxygen
species
(ROS)
generation
cells,
cellular
via
mitochondrial
intrinsic
pathway
was
confirmed.
inhibited
both
colony
formation
migration
ability,
highlighting
its
potential
an
anticancer
agent.
Additionally,
this
study
demonstrates
enhanced
expression
genes
BAX
accumulation
decreased
Bcl-2
gene
through
p53
signaling
pathway.
Furthermore,
research
on
properties
revealed
strong
NO
inhibition
well
inflammatory
mediators
(TNF-α,
iNOS,
COX-2,
IL-6,
IL-8)
NF-κB
However,
H2O2-induced
HaCaT
extract
reduced
stress
by
upregulating
level
antioxidative
markers
(SOD,
CAT,
GPx,
GST)
at
50
μg/mL.
As
result,
justifies
investigation
animal
clinical
trials
possible
source
antioxidants,
substances,
anti-cancer
compounds.
Journal of Functional Foods,
Journal Year:
2024,
Volume and Issue:
117, P. 106240 - 106240
Published: May 20, 2024
Recent
studies
have
highlighted
the
potential
of
unsaturated
fatty
acids
in
natural
oils
to
influence
virus
replication.
This
study
extracted
ginseng
seed
oil
(GSO)
and
evaluated
its
physiochemical
parameters,
lipid
class,
oxidation
stability.
GSO
was
then
synthesized
into
an
oil/water
nanoemulsion
(NE)
through
optimized
formulation,
time,
temperature,
storage
conditions.
GSO-NE
showed
no
cytotoxicity
up
5
µg/mL
but
effectively
inhibited
HCoV-OC43
replication
suppressed
proinflammatory
biomarkers
infected
cells.
RNA
sequencing
revealed
significant
alterations
gene
expression
profile
by
treatment,
particularly
implicating
ABC
transporters
signaling
pathway.
Notably,
downregulated
ABCG
subfamily
membrane
proteins,
crucial
for
regulation
virus-infected
The
downregulation
these
genes
(ABCG1,
ABCG2,
ABCG4,
ABCG5/8)
reversed
GSO-NE,
as
demonstrated
qRT-PCR.
Furthermore,
ABCG1
ABCG5/8
confirmed
using
immunoblotting
techniques.
These
findings
suggest
a
promising
avenue
future
research
or
therapeutic
applications.
Scientia Horticulturae,
Journal Year:
2023,
Volume and Issue:
322, P. 112388 - 112388
Published: Aug. 8, 2023
Ginsenosides,
as
the
main
pharmacological
activity
ingredients
of
ginseng,
can
be
used
to
treat
various
diseases.
Different
cultivars
ginseng
have
been
developed
through
domestication
and
artificial
selection.
However,
it
is
still
unclear
whether
there
are
differences
in
content
ginsenosides
regulatory
mechanisms
involved
among
cultivars.
In
this
study,
roots,
stems,
leaves
different
were
analyzed
for
transcriptome
data.
The
results
showed
that
total
was
higher
than
roots
moreover,
numerous
monomers
varied
significantly
Among
105
synthesis
genes,
most
UDP-glycosyltransferases
(UGTs)
highly
expressed
while
genes
from
2-C-methyl-d-erythritol-4-phosphate
(MEP)
pathway
overexpressed
leaves.
A
large
number
differentially
alternative
splicing
(DASGs)
found
comparison
groups,
overlapping
DEGs
DASGs
rare,
furthermore,
functions
various,
suggesting
(AS)
differential
expression
might
play
roles.
series
AS
events
Weighted
Gene
Co-Expression
Network
Analysis
(WGCNA)
constructed
analyze
gene-ginsenosides
association
gene
network.
Then,
extensive
transcription
factors
(TFs)
identified
correlated
modules,
including
AP2/ERF,
WRKY,
MYB,
bHLH
et
al.,
levels
these
TFs
with
genes.
Therefore,
influenced
structure
TFs,
respectively,
thereby
modulating
accumulation
ginsenosides.
provided
a
theoretical
basis
investigating
mechanism
ginsenoside
offered
new
perspective
development
natural
medicines.
