Selection and Multiplexing of Reverse Transcription–Quantitative PCR Tests Targeting Relevant Honeybee Viral Pathogens
Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(6), P. 1105 - 1105
Published: May 29, 2024
Verifying
the
inclusivity
of
molecular
detection
methods
gives
indications
about
reliability
viral
infection
diagnosis
because
tendency
pathogens
to
undergo
sequence
variation.
This
study
was
aimed
at
selecting
inclusive
probes
based
on
reverse
transcription–quantitative
PCR
(RT-qPCR)
assays
for
most
widespread
and
detrimental
viruses
infecting
honeybees,
namely
acute
bee
paralysis
virus
(ABPV),
black
queen
cell
(BQCV),
chronic
(CBPV),
deformed
wing
variants
A
(DWVA)
B
(DWVB),
sacbrood
(SBV).
Therefore,
previously
described
were
re-evaluated
in
silico
their
specificity
inclusivity.
Based
this
evaluation,
selected
modified,
or
new
ones
designed
tested
duplex
RT-qPCR
reactions.
The
limits
(LODs),
effect
multiplexing
sensitivity
RNA
quantification
potential
bees
hive
debris
assessed.
made
available
diagnostic
able
detect
an
increased
number
compared
with
tests
two
a
single
reaction.
Language: Английский
Selection and Multiplexing of Reverse Transcription Quantitative PCR Tests Targeting Relevant Honeybee Viral Pathogens
Published: May 6, 2024
Verification
of
inclusivity
molecular
detection
methods
gives
indications
on
the
reliability
viral
infections
diagnosis
because
tendency
pathogens
to
undergo
sequence
variation.
This
study
was
aimed
select
inclusive
probe
based
reverse
transcription
quantitative
PCR
(RT-qPCR)
assays
for
most
widespread
and
detrimental
viruses
infecting
honeybees,
namely
acute
bee
paralysis
virus
(ABPV),
black
queen
cell
(BQCV),
chronic
(CBPV),
deformed
wing
variants
A
(DWVA)
B
(DWVB),
sacbrood
(SBV).
Therefore,
previously
described
were
re-evaluated
in
silico
specificity
inclusivity.
Based
this
evaluation,
selected
modified
or
new
ones
designed
tested
duplex
RT-qPCR
reactions.
The
limits
(LODs),
effect
multiplexing
sensitivity
RNA
quantification
potential
bees
hive
debris
assessed.
made
available
diagnostic
able
detect
an
increased
number
compared
tests
two
a
single
reaction.
Language: Английский
New ecological insights on wild pollinator Andrena hesperia
Journal of Pollination Ecology,
Journal Year:
2024,
Volume and Issue:
37, P. 303 - 325
Published: Dec. 13, 2024
A
nesting
aggregation
of
Andrena
hesperia
in
Bologna
(Italy)
was
studied
to
characterize
this
bee’s
ecology,
phenology
and
interactions
with
the
environment.
adults
emerged
between
end
March
middle
April,
displaying
univoltine,
protandrous
phenology.
The
average
resistance
heat
stupor
A.
females
42.53
±
13.77
minutes
at
40°C.
Parasites
Nomada
facilis
Bombylius
canescens
were
associated
nests.
DNA
barcode
sequences
(COI
gene)
N.
sequenced
deposited
GenBank.
gut
microbiota
newly
individuals
dominated
by
Bacillota
(Lactobacillus
Fructobacillus)
Pseudomonadota
(Snodgrassella
alvei
Gilliamella).
pollen
carried
identified
morphologically
as
belonging
for
most
part
Asteraceae
family.
pathogens
detected
on
showed
different
infection
loads
newly-emerged
foraging
adults.
This
is
first
time
that
comprehensive
information
reported,
it
will
hopefully
foster
further
studies
wild
bee.
Language: Английский