Comparison of Conjugates Obtained Using DMSO and DMF as Solvents in the Production of Polyclonal Antibodies and ELISA Development: A Case Study on Bisphenol A DOI Creative Commons
Anna N. Berlina, Nadezhda S. Komova, Kseniya V. Serebrennikova

et al.

Antibodies, Journal Year: 2024, Volume and Issue: 13(4), P. 89 - 89

Published: Oct. 29, 2024

When developing immunochemical test systems, it is necessary to obtain specific antibodies. Their quality depends, among other things, on the immunogen used. preparing hapten–protein conjugates antibodies for low-molecular-weight compounds, key factors are structure of hapten itself, presence a spacer, size carrier protein and degree its modification by molecules. This work shows that one additional factor—the conditions obtaining conjugate—is overlooked. In this work, we have synthesized bisphenol A derivative 4,4-bis(hydroxyphenyl)valeric acid (BVA), soybean trypsin inhibitor (STI), bovine serum albumin (BSA) in reaction media combining water with two organic solvents: dimethylformamide (DMF) or dimethyl sulfoxide (DMSO). Namely, BSADMF–BVA, STIDMF–BVA, BSADMSO–BVA STIDMSO–BVA were obtained. Rabbit polyclonal against BSADMF–BVA conjugate demonstrated basically different interactions developed ELISA systems using either STIDMF–BVA conjugates. The use absence competition combination antisera obtained from an ELISA. competitive interaction was observed only conjugate. Under selected conditions, detection limit 8.3 ng/mL, working range determined concentrations 18.5–290.3 ng/mL. data demonstrate possibility achieving sensitive immunoassays simply varying conjugation, which could provide tool development compounds.

Language: Английский

A new ultrasonic triboelectric catalytic amplification strategy for SERS determination of trace nonylphenol in milk with dual nanosurface COF probes DOI
Zhiqiang Wang,

Chenguang Yi,

Guiqing Wen

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106712 - 106712

Published: May 1, 2025

Language: Английский

Citations

0
Immunodetection of Poorly Soluble Substances: Limitations and Their Overcoming DOI
Anna N. Berlina, Olga D. Hendrickson, Nadezhda S. Komova

et al.

Critical Reviews in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 26

Published: Oct. 3, 2024

Immunoassays based on the specific antigen-antibody interactions are efficient tools to detect various compounds and estimate their content. Usually, these assays implemented in water-saline media with composition close physiological conditions. However, many substances insoluble or cannot be molecularly dispersed such media, which objectively creates problems when interacting aquatic environments. Thus, obtaining immunoreactants implementing immunoassays of need special methodological solutions. Hydrophobicity antigens as well limited ability functionalization conjugation often overlooked developing for compounds. The main key finding is possibility influence behavior hydrophobic immunoassays, requires approaches summarized review. Using examples two groups compounds-surfactants (alkyl- bisphenols) fullerenes, we systematized existing knowledge experience development immunoassays. This review addresses challenges immunodetection poorly soluble proposes solutions use hydrotropes, other solubilization techniques, alternative receptors (aptamers imprinted polymers).

Language: Английский

Citations

0
Comparison of Conjugates Obtained Using DMSO and DMF as Solvents in the Production of Polyclonal Antibodies and ELISA Development: A Case Study on Bisphenol A DOI Creative Commons
Anna N. Berlina, Nadezhda S. Komova, Kseniya V. Serebrennikova

et al.

Antibodies, Journal Year: 2024, Volume and Issue: 13(4), P. 89 - 89

Published: Oct. 29, 2024

When developing immunochemical test systems, it is necessary to obtain specific antibodies. Their quality depends, among other things, on the immunogen used. preparing hapten–protein conjugates antibodies for low-molecular-weight compounds, key factors are structure of hapten itself, presence a spacer, size carrier protein and degree its modification by molecules. This work shows that one additional factor—the conditions obtaining conjugate—is overlooked. In this work, we have synthesized bisphenol A derivative 4,4-bis(hydroxyphenyl)valeric acid (BVA), soybean trypsin inhibitor (STI), bovine serum albumin (BSA) in reaction media combining water with two organic solvents: dimethylformamide (DMF) or dimethyl sulfoxide (DMSO). Namely, BSADMF–BVA, STIDMF–BVA, BSADMSO–BVA STIDMSO–BVA were obtained. Rabbit polyclonal against BSADMF–BVA conjugate demonstrated basically different interactions developed ELISA systems using either STIDMF–BVA conjugates. The use absence competition combination antisera obtained from an ELISA. competitive interaction was observed only conjugate. Under selected conditions, detection limit 8.3 ng/mL, working range determined concentrations 18.5–290.3 ng/mL. data demonstrate possibility achieving sensitive immunoassays simply varying conjugation, which could provide tool development compounds.

Language: Английский

Citations

0