Pleurotus ostreatus: Nutritional Enhancement and Antioxidant Activity Improvement Through Cultivation on Spent Mushroom Substrate and Roots of Leafy Vegetables

Fermentation, Journal Year: 2025, Volume and Issue: 11(1), P. 20 - 20

Published: Jan. 7, 2025

Agricultural residues, including Pleurotus ostreatus spent mushroom substrate (SMS) and roots of (hydroponic) leafy vegetables (RLV), were tested in various proportions as substrates new P. cultivations, where wheat straw was the control. The impact SMS RLV first evaluated by mycelial growth rate (Kr, mm/day). Afterwards, cultivation conducted bags, production characteristics like earliness (the time from inoculation to harvest) biological efficiency (BE %, ratio fresh weight dry × 100) examined. study also quality, nutritional composition bioactive content. highest intra-cellular polysaccharide (IPS) value (50.93%, w/w) observed containing 90%, while combining with resulted higher IPS values compared mixtures that included bran (WB) soybean flour (SF). Furthermore, use reduced fat content control substrate, except case 50%–RLV 40% produced mushrooms (4.68% w/w). Additionally, protein increased RLV. triterpene found 90%–RLV 10% (11.52 ursolic acid mg/g d.w.). However, exhibited fastest Kr (6.5 mm/d), well BE (87.8%) total phenolic compound (30.31 mg GAE/g Significant antioxidant activity all extracts, flavonoid low. Glucose dominant monosaccharide (over 51.5%, w/w), linoleic (18:2; over 57.05%, primary fatty across mushrooms. This enhances our knowledge which influence NFE2L2/HMOX-1 molecular pathway, thereby affecting specific antioxidant-related genes. These effects through carbohydrate extracts on LPS-challenged THP-1-derived macrophages. A positive gene expression HMOX1, CAT NFE2L2 during incubation aforementioned samples observed. findings support sustainable agricultural by-products cultivation, offering an environmentally friendly approach producing valuable products

Language: Английский

---

Exploring alternative sources of protein in food: Trends in nutrient and functional features

Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 116224 - 116224

Published: March 1, 2025

Language: Английский

---

Chemical Composition of Trametes versicolor, Hericium erinaceus, and Pleurotus ostreatus Mushroom Biomass: Deciphering Their Rich α-glucan Composition and Nutritional Value

Journal of Food Composition and Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 107612 - 107612

Published: April 1, 2025

Language: Английский

---

Impact of Spent Mushroom Substrate Combined with Hydroponic Leafy Vegetable Roots on Pleurotus citrinopileatus Productivity and Fruit Bodies Biological Properties

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1807 - 1807

Published: Sept. 1, 2024

Agricultural activities produce large quantities of organic byproducts and waste rich in lignocellulosic materials, which are not sufficiently utilized. In this study, alternative agricultural products, namely, spent mushroom substrate (SMS) from the cultivation edible Pleurotus ostreatus mushrooms roots leafy vegetables hydroponic (HRL), were evaluated for their potential to be used as substrates citrinopileatus effects on quality, nutritional value, chemical properties (lipid, protein, carbohydrate, ash, fatty acid carbohydrate composition) bioactive content (total phenolic compounds antioxidant activity) produced mushrooms. SMS HRL (in different ratios with without additives) wheat straw additives (WS—control) used. During incubation, linear growth rate mycelium (Kr, mm/day) was measured screening. Mushroom took place bags, where several characteristics examined: earliness (duration between day inoculation first harvest) biological efficiency (B.E. %, ratio weight fresh per dry × 100). Furthermore, study aimed investigate effect protein extract (PE) (CE) P. after vitro digestion (fraction less than 3kDa: PE-DP-3; digestate fraction: CE-D, respectively) expression antioxidant-related genes THP-1 cell line. The results showed that grown 50%-HRL 40% had fastest (6.1 mm/d) highest lipid contents (34.7% d.w.; 5.1% d.w.). B.E. (73.5%), total (65.7%) compound (60.2 mg GAE/g d.w.) values recorded control substrate. Antioxidant activity observed all extracts; flavonoid low samples, maximum triterpene value detected 80%-HRL 20% (9.8 UA/g mushrooms, linoleic (C18:2) main (above 60%), fructose dominant individual saccharide. investigation regulation pathway, NFE2L2 gene upregulated only 60%-HRL intervention during incubation CE-D samples. Additionally, transcription levels genes, SOD1, CAT, HMOX1 GSR, increased 60–30% intervention. Compared WS, trigger a pathway concerning CE may resist oxidative stress. This supports utilization through sustainable environmentally friendly practices while simultaneously producing high-value-added products such Therefore, substrates, particularly those containing HRL, could serve natural sources potential.

Language: Английский

---

Bio-Valorization of Spent Coffee Grounds and Potato Peel as Substrates for Pleurotus ostreatus Growth

Foods, Journal Year: 2024, Volume and Issue: 13(23), P. 3774 - 3774

Published: Nov. 25, 2024

Pleurotus ostreatus, due to its saprophytic nature, can extract nutrients and bioactive compounds from the substrate on which it is grown. This study aimed assess effect of adding spent coffee grounds (SCG) potato peel (PPW) in wheat straw formulation grow over production indicators, physicochemical, techno-functional, total chemical compounds, antioxidant properties. Treatments were described as follows: T1, at 100%; T2, 80% + 10% SCG PPW; T3, 70% 15% T4, 60% 20% PPW. After P. ostreatus growth, non-differences found indicators for T1–T4, including biological efficiency, rate, yield. With respect dried powders, T1–T4 showed pH values near neutrality concerning soy protein (SP), color samples beige. Also, T2 T3 exert higher water-holding (WHC) values, while oil-holding (OHC) emulsifying capacity (EC) SP, dependence growth substrate. lower swelling (SC) T1–T3 gelling (GC) values. Regarding properties extracts, solvent extraction have an metabolite content antiradical reducing power The multivariate analysis revealed that water extracts highest tannin (TTC) protocatechuic acid contents (PAC), well (RCSA) (RPA) In conclusion, this demonstrated using PPW a partial substitute (what straw) enhances activity ostreatus.

Language: Английский

---