Assessment of in vitro skin permeation and accumulation of phenolic acids from honey and honey-based pharmaceutical formulations
BMC Complementary Medicine and Therapies,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: Feb. 4, 2025
Honey
has
been
successfully
used
in
wound
care
and
cosmetics
because
of
its
effective
biological
properties,
including
antibacterial,
antioxidant,
anti-inflammatory
activities.
Polyphenols,
particularly
phenolic
acids,
are
key
honey
components
responsible
for
these
beneficial
effects.
In
recent
years,
there
a
growing
demand
natural,
ecologically
friendly,
biodegradable
products
the
modern
market.
This
study
aimed
to
identify
quantify
acids
four
Polish
samples
different
botanical
origins
(heather,
buckwheat,
linden
rapeseed)
assess
first
time
permeation
identified
through
skin
their
accumulation
after
application
pure
samples,
as
well
honey-based
hydrogel
emulsion
formulations.
The
samples'
antioxidant
activity
total
content
were
determined
using
DPPH
ABTS
assays
Folin-Ciocalteu
method,
respectively.
Phenolic
volatile
compounds
quantified
HPLC-UV
GC-MS
biocompatibility
was
evaluated
murine
fibroblast
cell
line
(L929).
A
Franz-type
vertical
diffusion
with
porcine
acid's
from
pharmaceutical
biodegradability
prepared
formulations
also
characterised.
Gallic
acid,
3,4-dihydroxybenzoic
2,5-dihydroxybenzoic
coumaric
3-hydroxybenzoic
acid
samples.
Heather
exhibited
significantly
higher
polyphenol
than
other
Heather,
buckwheat
decreased
viability
at
concentrations
5%
2.5%,
while
rapeseed
sample
markedly
reduced
only
5%.
Among
tested
-
honey,
hydrogel,
rates
observed
hydrogels
honeys
emulsions.
Additionally,
classified
partially
biodegradable.
obtained
results
confirmed
effectiveness
two
form
or
containing
applied
topically.
inclusion
vehicle,
particular
increased
penetration
skin.
Language: Английский
Chemical Composition, Antioxidant Potential, and Antimicrobial Activity of Novel Antiseptic Lotion from the Leaves of Betula pendula Roth
Applied Sciences,
Journal Year:
2025,
Volume and Issue:
15(7), P. 3658 - 3658
Published: March 26, 2025
Plants
of
the
genus
Betula
pendula
(Betulaceae)
have
been
used
as
antiseptics
in
treatment
skin
diseases.
While
bark
this
plant
has
so
far
a
medicinal
raw
material,
our
research
revealed
that
leaves
are
an
extremely
valuable
source
health-promoting
bioactive
compounds,
especially
polyphenolic
compounds.
The
antioxidant
activity
novel
lotion
from
leaf
and
its
phenolic
content
was
determined
using
DPPH
FRAP
assays
Folin–Ciocalteu
method,
qualitative
analysis
conducted
GC-MS
method.
antiseptic
preparation
also
subjected
to
HPLC
for
assessment
acids
(gallic,
3,4-dihydroxybenzoic,
2,5-dihydroxybenzoic,
chlorogenic,
3-hydroxybenzoic
acids).
shake
flask
method
determine
partition
coefficient
assess
lipophilicity.
A
new
ethanol-reduced
containing
antimicrobial
ingredients
evaluated
standard
killing
methods
(EN
13697:2019
EN
13697:2015)
at
60
±
10
s
300
against
six
microorganisms.
aim
these
tests
whether
formulation
exhibited
performance
like
traditional
ethanol.
In
addition,
hand
disinfection
were
carried
out
obtained
B.
accordance
with
1500:2013,
Escherichia
coli
strain
K12
NCTC
10538.
According
results,
high
polyphenol
contents
(52
2
mg
GAE/g
dry
material)
other
biologically
active
substances
identified
by
(phytol,
alpha-tocospiro
B,
sitosterol,
or
dilauryl
thiodipropionate)
can
be
thought
parameters
responsible
effective
derived
birch
leaves.
Gallic
acid
3,4-dihydroxybenzoic
had
greatest
concentrations
among
examined.
mean
kill
time
>99.9
Staphylococcus
aureus
ATCC
6538
(at
concentration
30
g/100
mL)
Pseudomonas
aeruginosa
15442
40
mL),
while
ethanol
showed
same
reduction
microorganisms
80
mL.
Language: Английский