Comparative Analysis of Sustainable Extraction Methods and Green Solvents for Olive Leaf Extracts with Antioxidant and Antihyperglycemic Activities DOI Creative Commons
Nils Leander Huamán-Castilla, Luis Omar Mamani-Apaza, Franz Zirena Vilca

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1523 - 1523

Published: Dec. 12, 2024

Olive leaves are agro-industrial waste that pose an environmental management problem. However, they contain polyphenolic compounds with important bioactive properties beneficial to human. This study aimed evaluate the effectiveness of two extraction technologies (pressurized liquid and ultrasound-assisted extraction) combined green solvents (pure water, 15% ethanol, glycerol) at 50 °C 70 °C. The goal was obtain extracts both antioxidant capacity antihyperglycemic from olive leaves. Pressurized glycerol most effective method for obtaining rich in total polyphenols (19.46 mg GAE/g dw), 4.11 mg/mL (inhibition capacity: IC50) 500.26 µmol TE/g dw. For methods °C, more recovering phenolic acids, stilbenes secoiridoid; while ethanol flavonols phenylethanoids. Oleuropein polyphenol extracted pressurized extractions, concentrations 171.48 µg/g dw 246.70 dw, respectively. extract obtained exhibited significant inhibition (70%) α-glucosidase enzymes, similar reference drug acarbose. In contrast, these showed low inhibitory activity against α-amylase enzyme. These findings can be applied development functional foods nutraceutical supplements managing postprandial glycemic response, offering a natural alternative supporting type 2 diabetes management.

Language: Английский

From Waste to Value: Solubility and Dissolution Enhancement of Bioactive Extracts from Olive Leaves Using Poloxamers DOI Creative Commons
Muhammad Wasim, Maria Camilla Bergonzi

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 928 - 928

Published: Feb. 17, 2025

The European Union, producing over 2.5 billion tons of waste annually, has prompted the Parliament to implement legal measures and encourage shift towards a circular economy. Millions biowaste from olive plant leaves are generated resulting in environmental economic challenges. To address this, was valorized, extraction valuable components, triterpenes polyphenols, which hold potential pharmaceutical, food, or cosmetic applications. Our research involved formulation triterpene extract (TTP70, 70% triterpenes) as solid dispersion using Poloxamer-188 (P188) Poloxamer-407 (P407). dispersions were prepared kneading method various extract-to-polymer weight ratios, including 1:1, 1:2, 1:5. influence hydrophilic carriers on solubility, dissolution profile, vitro passive permeability TTP70 evaluated. Both all considered ratios significantly improved solubility hydrophobic triterpenes. PAMPA experiments demonstrated efficacy improving permeation Subsequently, physically mixed with polyphenol-enriched (OPA40, 49% polyphenols) also obtained leaves, they used fill hard gelatin capsules produce an oral dosage form. composite formulations both classes constituents.

Language: Английский

Citations

0
Consequences of Dietary Olive Leaf Powder Supplementation on Growth Performance, Carcass Traits, Blood Biochemical Parameters and Gut Microbiota in Broilers DOI Creative Commons

Mohamed H. Negm,

Ahmed Khalid Aldhalmi,

Elwy A. Ashour

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105087 - 105087

Published: March 1, 2025

This experiment examined the potential of olive leaf powder (OLP) as a natural growth enhancer in broiler feed and its influences on performance, carcass characteristics, blood parameters, intestinal bacterial count. A total 210 one-day-old Arbor Acres chicks were randomly allocated into three groups. The control group was given basal diet only, 2nd 3rd groups supplemented with 1 g OLP 2 OLP/kg diet, respectively. Each consisted seven replicates 10 unsexed each. experimental trial lasted for thirty-one days. Results showed that, OLP2 exhibited significant increase live body weight (LBW) at days 14 21 age, whereas OLP1 experienced considerable rise gain (BWG) between 29 31 age. Average daily intake (ADFI) considerably decreased during first 8-14 trial, but increased next 29-31 throughout entire duration (1-31 days). While, Feed conversion ratio (FCR) improved 15-31 trial. Carcass breast yields significantly supplementation, while abdominal fat content reduced. Blood analysis revealed enhances protein, albumin, globulin levels both groups, alanine transaminase (ALT), creatinine, cholesterol (TC), triglycerides (TG), very low-density lipoprotein (VLDL) Notably, immunity antioxidant markers improvement supplementation. Due to number beneficial bacteria such Lactobacillus rose all harmful (E. coli Clostridium) caecal samples declined. In conclusion, supplementation per kg demonstrated positive impact quality, lipid profile, immunity, status, raised contents chickens, making it promising promoter poultry production.

Language: Английский

Citations

0
Protective effect of oleuropein on the brain tissue in D-Galactose-induced aging in rat model DOI
Xiao Hu, Nan Zhao,

Elham Ranjbar

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 52(1)

Published: Dec. 20, 2024

Language: Английский

Citations

1
Comparative Analysis of Sustainable Extraction Methods and Green Solvents for Olive Leaf Extracts with Antioxidant and Antihyperglycemic Activities DOI Creative Commons
Nils Leander Huamán-Castilla, Luis Omar Mamani-Apaza, Franz Zirena Vilca

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1523 - 1523

Published: Dec. 12, 2024

Olive leaves are agro-industrial waste that pose an environmental management problem. However, they contain polyphenolic compounds with important bioactive properties beneficial to human. This study aimed evaluate the effectiveness of two extraction technologies (pressurized liquid and ultrasound-assisted extraction) combined green solvents (pure water, 15% ethanol, glycerol) at 50 °C 70 °C. The goal was obtain extracts both antioxidant capacity antihyperglycemic from olive leaves. Pressurized glycerol most effective method for obtaining rich in total polyphenols (19.46 mg GAE/g dw), 4.11 mg/mL (inhibition capacity: IC50) 500.26 µmol TE/g dw. For methods °C, more recovering phenolic acids, stilbenes secoiridoid; while ethanol flavonols phenylethanoids. Oleuropein polyphenol extracted pressurized extractions, concentrations 171.48 µg/g dw 246.70 dw, respectively. extract obtained exhibited significant inhibition (70%) α-glucosidase enzymes, similar reference drug acarbose. In contrast, these showed low inhibitory activity against α-amylase enzyme. These findings can be applied development functional foods nutraceutical supplements managing postprandial glycemic response, offering a natural alternative supporting type 2 diabetes management.

Language: Английский

Citations

0