Limosilactobacillus fermentum MG4244 Protects Against Metabolic and Inflammatory Stress in Caenorhabditis elegans DOI Creative Commons

Yebin Kim,

Opeyemi Deji-Oloruntoba,

Young-Kyun Choe

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(11), P. 1995 - 1995

Published: June 5, 2025

In this study, we investigated the effects of MG4244 on intestinal permeability, oxidative stress, and lipid accumulation in Caenorhabditis elegans with metabolic inflammation induced by Pseudomonas aeruginosa (PA) a high-glucose diet (HGD). The worms infected PA exhibited increased permeability reactive oxygen species (ROS) production, which were improved upon treatment. Also, inhibited ROS an HGD. addition, MG4244-treated showed extended lifespans under various conditions. To elucidate mechanism effects, conducted further investigation using mutant strains knockdown genes associated AMP-activated protein kinase (AMPK) mitogen-activated (MAPK) pathways. results demonstrated that effect metabolism was primarily mediated through AMPK signaling pathway. Furthermore, enhanced pathogen resistance MAPK pathways, mitigating stress responses, maintaining integrity. studies, combined treatment HGD significantly lipid, levels, confirming their negative synergistic effects. However, co-treatment conditions effectively mitigated these health disruptions, suggesting protective role MG4244. This study provides vivo platform C. to evaluate probiotic efficacy related environment. our highlight therapeutic potential improving resilience gut-targeted mechanisms.

Language: Английский

Limosilactobacillus fermentum MG4244 Protects Against Metabolic and Inflammatory Stress in Caenorhabditis elegans DOI Creative Commons

Yebin Kim,

Opeyemi Deji-Oloruntoba,

Young-Kyun Choe

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(11), P. 1995 - 1995

Published: June 5, 2025

In this study, we investigated the effects of MG4244 on intestinal permeability, oxidative stress, and lipid accumulation in Caenorhabditis elegans with metabolic inflammation induced by Pseudomonas aeruginosa (PA) a high-glucose diet (HGD). The worms infected PA exhibited increased permeability reactive oxygen species (ROS) production, which were improved upon treatment. Also, inhibited ROS an HGD. addition, MG4244-treated showed extended lifespans under various conditions. To elucidate mechanism effects, conducted further investigation using mutant strains knockdown genes associated AMP-activated protein kinase (AMPK) mitogen-activated (MAPK) pathways. results demonstrated that effect metabolism was primarily mediated through AMPK signaling pathway. Furthermore, enhanced pathogen resistance MAPK pathways, mitigating stress responses, maintaining integrity. studies, combined treatment HGD significantly lipid, levels, confirming their negative synergistic effects. However, co-treatment conditions effectively mitigated these health disruptions, suggesting protective role MG4244. This study provides vivo platform C. to evaluate probiotic efficacy related environment. our highlight therapeutic potential improving resilience gut-targeted mechanisms.

Language: Английский

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