Metaproteomic Analysis of the Anaerobic Community Involved in the Co-Digestion of Residues from Sugarcane Ethanol Production for Biogas Generation DOI Creative Commons
Maria Paula Cardeal Volpi,

Larissa O. Magalhães,

Flávia Vischi Winck

et al.

Biomass, Journal Year: 2022, Volume and Issue: 2(4), P. 358 - 373

Published: Dec. 5, 2022

The proteomics analysis could contribute to better understand about metabolic pathways in anaerobic digestion community because it still as a “black-box” process. This study aimed analyze the proteins of co-digestion performed reactors containing residues from first and second generation ethanol production. Metaproteomics was carried out for three types samples: sludge without substrate (SI), semi-continuous stirred reactor (s-CSTR) with filter cake, vinasse, deacetylation liquor (R-CoAD) s-CSTR these aforementioned adding Fe3O4 nanoparticles (R-NP). R-CoAD achieved 234 NmLCH4 gVS−1 65% CH4 biogas, while R-NP reached 2800 80% CH4. main found were enolase, xylose isomerase, pyruvate phosphate dikinase, different proportion each sample, indicating some change pathways. However, according those identified proteins, routes involved syntrophic acetate oxidation coupled hydrogenotrophic methanogenesis, production occurring preferentially via CO2 reduction. These findings contributed unravel at micromolecular level, may select more appropriate inoculum biogas that residue, reducing reaction time increasing productivity.

Language: Английский

Metaproteomic Analysis of the Anaerobic Community Involved in the Co-Digestion of Residues from Sugarcane Ethanol Production for Biogas Generation DOI Creative Commons
Maria Paula Cardeal Volpi,

Larissa O. Magalhães,

Flávia Vischi Winck

et al.

Biomass, Journal Year: 2022, Volume and Issue: 2(4), P. 358 - 373

Published: Dec. 5, 2022

The proteomics analysis could contribute to better understand about metabolic pathways in anaerobic digestion community because it still as a “black-box” process. This study aimed analyze the proteins of co-digestion performed reactors containing residues from first and second generation ethanol production. Metaproteomics was carried out for three types samples: sludge without substrate (SI), semi-continuous stirred reactor (s-CSTR) with filter cake, vinasse, deacetylation liquor (R-CoAD) s-CSTR these aforementioned adding Fe3O4 nanoparticles (R-NP). R-CoAD achieved 234 NmLCH4 gVS−1 65% CH4 biogas, while R-NP reached 2800 80% CH4. main found were enolase, xylose isomerase, pyruvate phosphate dikinase, different proportion each sample, indicating some change pathways. However, according those identified proteins, routes involved syntrophic acetate oxidation coupled hydrogenotrophic methanogenesis, production occurring preferentially via CO2 reduction. These findings contributed unravel at micromolecular level, may select more appropriate inoculum biogas that residue, reducing reaction time increasing productivity.

Language: Английский

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