A pilot-scale aerobiology chamber to investigate bioaerosol control agents: Factors affecting longevity of aerosolized pathogenic and nonpathogenic bacteria DOI Creative Commons

David R. Kasler,

Mohamed M. Ali, Banothu Ramji

et al.

Aerosol Science and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 19

Published: Dec. 13, 2024

Driven by the need for pilot-scale aerobiology chambers, we constructed and tested a free-standing 0.5-m3 cuboid chamber, complete with accessories needed to control experimental variables, measure treatment parameters, sample treated untreated air. Parameters affecting bioaerosol longevity were tested; these included target bacterium species their initial population counts, relative humidity, presence of hot spots within chamber. When 2 mL (108–109 CFU/mL) cell suspensions bacteria aerosolized, airborne concentration in chamber averaged 4.3 (± 0.28 SD) log10 CFU/L-air immediately after nebulization, indicating acceptable transferability cells from liquid medium into aerosolized state. A sizable portion this retained viability chamber; average decay during 40-min aerosol holding was 0.836 0.498 CFU/L-air. The likely attributed both biological physical losses. Aerosols Staphylococcus aureus ATCC 6583 Pseudomonas aeruginosa 10145 significantly (p < 0.05) more stable than those Escherichia coli K12 Klebsiella pneumoniae 556. compared other species, S. produced most aerosols 40-minute hold. Longevity increased increasing nebulizer high humidity. Chamber air temperature did not affect longevity, but introduction (>200 °C) inside greatly affected recovery viable cells. In conclusion, developed midsize successfully used investigate stability, it is potentially useful studying agents.

Language: Английский

Impact of Cultivating Different Ocimum Species on Bioaerosol Bacterial Communities and Functional Genome at an Agricultural Site DOI
Anisha Yadav,

Ranu Yadav,

Puja Khare

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 356, P. 124289 - 124289

Published: May 31, 2024

Language: Английский

Citations

2
A pilot-scale aerobiology chamber to investigate bioaerosol control agents: Factors affecting longevity of aerosolized pathogenic and nonpathogenic bacteria DOI Creative Commons

David R. Kasler,

Mohamed M. Ali, Banothu Ramji

et al.

Aerosol Science and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 19

Published: Dec. 13, 2024

Driven by the need for pilot-scale aerobiology chambers, we constructed and tested a free-standing 0.5-m3 cuboid chamber, complete with accessories needed to control experimental variables, measure treatment parameters, sample treated untreated air. Parameters affecting bioaerosol longevity were tested; these included target bacterium species their initial population counts, relative humidity, presence of hot spots within chamber. When 2 mL (108–109 CFU/mL) cell suspensions bacteria aerosolized, airborne concentration in chamber averaged 4.3 (± 0.28 SD) log10 CFU/L-air immediately after nebulization, indicating acceptable transferability cells from liquid medium into aerosolized state. A sizable portion this retained viability chamber; average decay during 40-min aerosol holding was 0.836 0.498 CFU/L-air. The likely attributed both biological physical losses. Aerosols Staphylococcus aureus ATCC 6583 Pseudomonas aeruginosa 10145 significantly (p < 0.05) more stable than those Escherichia coli K12 Klebsiella pneumoniae 556. compared other species, S. produced most aerosols 40-minute hold. Longevity increased increasing nebulizer high humidity. Chamber air temperature did not affect longevity, but introduction (>200 °C) inside greatly affected recovery viable cells. In conclusion, developed midsize successfully used investigate stability, it is potentially useful studying agents.

Language: Английский

Citations

0