Annals of Biomedical Engineering, Journal Year: 2024, Volume and Issue: 53(2), P. 284 - 317
Published: Oct. 14, 2024
Language: Английский
MedComm, Journal Year: 2025, Volume and Issue: 6(2)
Published: Jan. 26, 2025
Abstract Airway stenosis (AS) is a fibroinflammatory disease characterized by abnormal activation of fibroblasts and excessive synthesis extracellular matrix, which has puzzled many doctors despite its relatively low prevalence. Traditional treatment such as endoscopic surgery, open adjuvant therapy have disadvantages are limited in the patients with recurrent AS. Therefore, it urgent to reveal pathogenesis AS accelerate clinical transformation. Based on discovered pathogenesis, including fibrosis, inflammation, epithelial–mesenchymal transition, metabolic reprogramming, microbiome, genetic susceptibility, other mechanisms, researchers developed series treatments, drug therapy, gene stem cell growth factor protein photodynamic therapy. This review introduces classification AS, explores existing preclinical treatments based finally summarizes current management. In addition, prospect exploring interaction between different types cells microorganisms identify intersection multiple mechanisms single‐cell RNA sequencing, 16S rRNA sequencing shotgun metagenomic worth looking forward to.
Language: Английский
Citations
1
SLAS TECHNOLOGY, Journal Year: 2025, Volume and Issue: unknown, P. 100256 - 100256
Published: Feb. 1, 2025
The automation of biofabrication processes has the potential to increase both scale and reproducibility human tissue production for replacing animal usage in research ultimately clinical use. technology, Rotational Internal Flow Layer Engineering (RIFLE), produces layered tubular constructs with a resolution commensurate microscale strata observed many types. previously published RIFLE process required liquid phase cell-laden hydrogels be manually applied onto inner surface high-speed rotating mould. Here we describe improvement system by automating elements process, particular dispensing element, present use this two commonly used hydrogels; alginate collagen. Semi-automatically assembled cell layers matched viabilities those produced manually, automated collagen demonstrating highest (>91%) over 10 days measured, highlighting its advantages as material engineering applications. encapsulation labelled cells predefined layer patterns confirmed that semi-automated was able assemble separate populations cell-width (≈14µm). Semi-automated dosing reduced manual operations reducing workload on researchers minimising opportunity error. Further exists higher levels overall particularly needed preparation cell-hydrogel suspensions, common labour-intensive technologies.
Language: Английский
Citations
0
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 25, 2025
Engineering hollow fibers with precise surface microstructures is challenging; yet, essential for guiding cells alignment and ensuring proper vascular tissue function. Inspired by Euplectella sponges, a novel strategy to engineer biomimetic spiral developed. Using oxidized bacterial cellulose, polydopamine, "brick-and-mortar" scaffold created through shear control during microfluidic coaxial spinning. The mimics natural extracellular matrices, providing mechanical stability supporting cell growth. In vitro studies show successful co-culture of endothelial (ECs) smooth muscle (SMCs), SMCs aligning along ECs forming confluent inner layer. vivo implantation confirms biocompatibility, biodegradability, low immunogenicity. This Euplectella-inspired presents promising approach engineering regenerative medicine.
Language: Английский
Citations
0
Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13
Published: Feb. 28, 2025
Certain congenital or acquired diseases and defects such as tracheo-oesophageal fistula, tracheomalacia, tracheal stenosis, airway ischemia, infections, tumours can cause damage to the trachea. Treatments available do not offer any permanent solutions. Moreover, long-segment in trachea have no surgical treatments. Tissue engineering has gained popularity current regenerative medicine a promising approach bridge this gap. Among various tissue techniques, decellularization is widely used that removes cellular nuclear contents from while preserving native extracellular matrix components. The decellularized scaffolds exhibit significantly lower immunogenicity retain essential biomechanical proangiogenic properties of tissue, creating foundation for regeneration. present review provides an overview advancements, exploring how recellularization approaches be optimized by using stem cells tissue-specific restore scaffold’s structure function. We examine critical factors mechanical properties, revascularization, involved transplantation tissue-engineered grafts.
Language: Английский
Citations
0
Deleted Journal, Journal Year: 2025, Volume and Issue: 7(4)
Published: March 20, 2025
Language: Английский
Citations
0
Regenerative Therapy, Journal Year: 2025, Volume and Issue: 29, P. 364 - 380
Published: April 9, 2025
Language: Английский
Citations
0
Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown
Published: April 14, 2025
Abstract The study presents an elastic, rigid, and shapeable silk fibroin (SF)‐based scaffold with a dual‐crosslinked network for tracheal tissue engineering. Hemoglobin (Hb), biocompatible cost‐effective natural protein, is introduced as both catalyst crosslinker. in situ chemical crosslinking occurred via dityrosine reaction the presence of hydrogen peroxide. Subsequently, freeze‐drying induced formation β‐sheet crystals, further physically SF chains, resulting 3D porous Hb‐SF scaffold. Additionally, α‐helix subunits Hb acted “molecular springs”, enhancing scaffold's mechanical strength, elasticity, toughness. biomechanical properties are tunable by adjusting content, enabling precise shape fidelity, self‐recovery, durability. Notably, exhibited high fatigue resistance, enduring multiple compressive cycles without damage. In vitro tests confirmed its cytocompatibility potential cartilage regeneration. Furthermore, vivo implantation chondrocyte‐seeded scaffolds resulted biomimetic trachea structural closely resembling those native trachea. This Hb‐mediated, demonstrates enhanced rigidity, toughness, shapeability, presenting viable option regeneration applications.
Language: Английский
Citations
0
IGI Global eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 311 - 334
Published: April 25, 2025
Tissue engineering, stem cells, and cloning are just a few of the technological fields that falls under regenerative medicine. engineering techniques utilize cellular matrices, which rely on body's inherent capacity for regeneration to ensure new tissue growth is properly oriented directed, use matrices containing cells. Biomaterials give cells three-dimensional environment in develop into tissues with right structure function. They can also facilitate distribution bioactive substances, such as factors cell adhesion peptides, intended locations. Current regulations ethical considerations advise before novel medicine technique or instrument implemented standard clinical practice used by numerous patients, controlled randomized trials must be carried out. Clinical studies involving treatments have revealed some restrictions, difficulties, unknowns.
Language: Английский
Citations
0
Polymers, Journal Year: 2024, Volume and Issue: 16(17), P. 2459 - 2459
Published: Aug. 29, 2024
Nanomaterials are known as the most promising materials of 21st century, among which nanofibers have become a hot research and development topic in academia industry due to their high aspect ratio, specific surface area, molecular orientation, crystallinity, excellent mechanical properties, many other advantages. Electrospinning is important preparation method for thin membranes its controllability, versatility, low cost, simplicity. Adding nanofillers such ceramics, metals, carbon electrospinning polymer solutions prepare composites can further improve strength multi-functionality also provide possibilities widespread applications. Based on rapid field composite nanofibers, this review focuses polyurethane (PU)-based main representative reviews latest practical applications fields sound-absorbing materials, biomedical (including tissue engineering implants, drug delivery systems, wound dressings anti-bacterial health etc.), wearable sensing devices energy harvesters, adsorbent electromagnetic shielding reinforcement materials. Finally, summary performance-application relationship prospects given. This expected some experience theoretical guidance developments related fields.
Language: Английский
Citations
2
Annals of Biomedical Engineering, Journal Year: 2024, Volume and Issue: 53(2), P. 284 - 317
Published: Oct. 14, 2024
Language: Английский
Citations
0