Leukocytes within Autologous Blood Concentrates Have No Impact on the Growth and Proliferation of Human Primary Osteoblasts: An In Vitro Study DOI Open Access
Carlos Fernando de Almeida Barros Mourão, Eva Dohle, Büşra Yaprak Bayrak

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4542 - 4542

Published: April 21, 2024

Platelet-rich fibrin (PRF) is a widely used autologous blood concentrate in regenerative medicine. This study aimed to characterize the cellular composition and distribution of different PRF matrices generated by high (710 g) low (44 relative centrifugal forces (RCFs) analyze their bioactivity on human primary osteoblasts (pOBs). was separated into upper layer (UL) buffy coat (BC) fractions, contents were assessed using histological immunohistochemical staining. The release platelet-derived growth factor (PDGF) transforming (TGF-β) quantified an ELISA. Indirect treatment pOBs performed evaluate cell viability morphology. A analysis revealed higher quantities leukocytes platelets low-RCF PRF. TGF-β significantly compared high-RCF All fractions promoted pOB proliferation regardless centrifugation protocol used. showed levels than These findings contribute understanding mechanisms provide insights optimizing protocols for bone regeneration, advancing medicine, improving patient outcomes.

Language: Английский

Temporomandibular Disorders Management—What’s New? A Scoping Review DOI Creative Commons
Giovanni Mauro,

Alessio Verdecchia,

Carlota Suárez-Fernández

et al.

Dentistry Journal, Journal Year: 2024, Volume and Issue: 12(6), P. 157 - 157

Published: May 23, 2024

Temporomandibular disorders include various conditions that impact the masticatory system, affecting its structure, function, or physiology. Clinicians face a complex array of therapeutic options when treating this group diseases, emphasizing importance evaluating current evidence to guide decisions in patient care. The main objective article is conduct scoping review on available treatment approaches manage temporomandibular (TMDs). An extensive search literature was performed Scopus, Pubmed, Embase, and Web Science. Systematic reviews published last 5 years were considered. Out 2183 publications identified, 109 studies included review. Among them, 39 articles focused non-invasive approach, while 120 delved into minimally invasive 15 explored surgical approach. Non-invasive conservative like cognitive-behavioral therapy, physical acupuncture offer effective pain management functional improvements TMDs. Emerging treatments promising alternatives for these disorders. Surgery should be reserved severe cases, with therapies used conjunction procedures optimal outcomes.

Language: Английский

Citations

7

Leukocytes within Autologous Blood Concentrates Have No Impact on the Growth and Proliferation of Human Primary Osteoblasts: An In Vitro Study DOI Open Access
Carlos Fernando de Almeida Barros Mourão, Eva Dohle, Büşra Yaprak Bayrak

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4542 - 4542

Published: April 21, 2024

Platelet-rich fibrin (PRF) is a widely used autologous blood concentrate in regenerative medicine. This study aimed to characterize the cellular composition and distribution of different PRF matrices generated by high (710 g) low (44 relative centrifugal forces (RCFs) analyze their bioactivity on human primary osteoblasts (pOBs). was separated into upper layer (UL) buffy coat (BC) fractions, contents were assessed using histological immunohistochemical staining. The release platelet-derived growth factor (PDGF) transforming (TGF-β) quantified an ELISA. Indirect treatment pOBs performed evaluate cell viability morphology. A analysis revealed higher quantities leukocytes platelets low-RCF PRF. TGF-β significantly compared high-RCF All fractions promoted pOB proliferation regardless centrifugation protocol used. showed levels than These findings contribute understanding mechanisms provide insights optimizing protocols for bone regeneration, advancing medicine, improving patient outcomes.

Language: Английский

Citations

2