Histomorphometric and immunohistochemical assessment of treated dentin matrix delivered by platelet-rich fibrin for socket preservation in rabbit model

BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 12, 2025

This study aimed to test treated dentine matrix (TDM) with platelet-rich fibrin (PRF) for socket preservation, following tooth extraction compared synthetic grafting material Nanobone (NB) PRF. Study was conducted on New Zealand rabbits (n = 40). Bilateral first lower premolar performed, one side left empty and the other filled in group 20). In 20), TDM/PRF while NB/ After three months, were euthanized, area examined using haematoxylin eosin (H&E) Goldner Masson trichrome stains. One-way ANOVA post-hoc tests used histomorphometric analysis. Immunohistochemical analysis of osteopontin carried out. Histological NB/PRF groups showed a higher level new bone formation comparison control PRF groups. Histomorphometric revealed significant increase after months (p 0.042 p < 0.001), respectively. There no differences percentages unmineralized between at both intervals 0.375 0.352, respectively). Regarding immunohistochemistry, highest immune expression followed by TDM/PRF. No detected 0.234 & 0.607 ability form sockets rabbits. can be as an alveolar preservation.

Language: Английский

---

Evaluation of the usefulness of platelet-rich fibrin (PRF) in mandibular third molar surgery with 3D facial swelling analysis: a split-mouth randomized clinical trial

Head & Face Medicine, Journal Year: 2025, Volume and Issue: 21(1)

Published: Feb. 22, 2025

Third molar surgery is associated with various postoperative complications (PC). Different strategies, including the application of platelet-rich fibrin (PRF), have been implemented to reduce PC. Digital technologies proven useful in objectively assessing facial swelling. This study aimed evaluate effect PRF on reducing swelling after lower third using a 3D face scanner. A randomized split-mouth clinical trial was set up and 32 patients (18 years), requiring extraction both mandibular molars, were recruited at Oral Surgery Clinic Magna Graecia University Catanzaro. The primary predictive variable or not plugs membranes post-extraction socket. Primary outcome recorded scanner preoperatively (T0), three (T1) seven (T2) days. Qualitative quantitative data analysis conducted following an automated standardized imaging workflow Slicer software. Secondary variables trismus, by measuring maximum buccal opening caliper, pain, visual analogue scale (VAS), duration surgery. Descriptive bivariate performed setting significance level $$\:\alpha\:$$ = 0.05. All exhibited significant increase T1, followed subsequent reduction from day 3 7, slight persistence edema observed seventh day. No emerged statistical conducted. Linear differences group reported improved values only T1-T2 T0-T2 phases analysis. Volumetric favored compared control all phases. VAS T2, group. Application sockets showed effectiveness Its advantages, accessibility, cost-effectiveness, absence adverse reactions, make it optimal treatment choice post-surgical sequelae.

Language: Английский

---

Efficacy of concentrated growth factor combined with coronally advanced flap in the treatment of gingival recession: a systematic review and meta-analysis

BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 9, 2025

This study aims to evaluate the efficacy of combining coronally advanced flap (CAF) technique with concentrated growth factor (CGF) in treatment gingival recession (GR), and compare this approach other alternative treatments. systematic review meta-analysis included randomized controlled trials (RCTs) comparing CAF combined CGF treatments for root coverage procedures. Included studies evaluated systemically healthy adults (> 18 years) Miller Class I/II or Cairo RT1 recessions. Primary outcomes were complete (CRC) mean (MRC); secondary changes keratinized tissue width (KTW), thickness (GT), clinical attachment level (CAL), (RW), depth (RD), probing (PD). A comprehensive search was conducted across multiple databases, including PubMed, Scopus, Cochrane Library, Web Science, Embase, up November 9, 2024. The protocol prospectively registered PROSPERO (CRD42024556815). Statistical analyses performed using Review Manager 5.4.1. Eight meta-analysis. Compared alone, combination significantly improved CRC (OR = 1.79, P 0.04), MRC (MD 10.38%, KTW 0.40 mm, 0.02), GT 0.26 < 0.00001), CAL 0.36 0.03). connective graft (CTG) showed superior compared + 0.25, 0.009). However, no significant differences found between CTG MRC, CAL, KTW, RD, RW, PD. Additionally, observed when PRF all parameters (all > 0.05). findings indicate that CAF/CGF improves treating GR may be a viable is not applicable. Further are needed validate GR. In cases where applicable, serve as class I II

Language: Английский

---

Does platelet‐rich fibrin enhance the outcomes of peri-implant soft tissues? A systematic review

BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 22, 2025

Language: Английский

---

First- vs. Second-Generation Autologous Platelet Concentrates and Their Implications for Wound Healing: Differences in Proteome and Secretome

Bioengineering, Journal Year: 2024, Volume and Issue: 11(11), P. 1171 - 1171

Published: Nov. 20, 2024

Differences in cell count and growth factor expression between first- second-generation autologous platelet concentrates (APCs) have been well described. The debate over which formula best supports wound healing various surgical procedures is still ongoing. This study aims to assess the whole proteome assembly, content, immunological potential pro-angiogenic of APC, Platelet-Rich Fibrin (PRF) vs. first-generation Plasma (PRP). global APCs was analyzed using nano-liquid chromatography mass spectrometry. Blood concentrations were determined by an automated counter. effect on macrophage polarization flow cytometry. A yolk sac membrane (YSM) assay used monitor neo-vessel formation capillary branching vivo. Cell analysis revealed a higher number/concentration leukocytes PRF PRP. Incubation macrophages with PRP or platelet-free plasma (PFP) did not induce significant pro-inflammatory state but led shift M0/M2 phenotype as seen for all tested formulas. Label-free proteomics identified total 387 proteins from three biological replicates respective designated groups. induced increased neo-vessels points vivo comparison PFP (each p < 0.001), indicating enhanced PRF. Overall, seems superior PRP, important representative Inclusion leucocytes compared suggested rather anti-inflammatory macrophages. These results are support versatile clinical applications regenerative medicine optimize healing.

Language: Английский

---

Effect of Liquid Blood Concentrates on Cell Proliferation and Cell Cycle- and Apoptosis-Related Gene Expressions in Nonmelanoma Skin Cancer Cells: A Comparative In Vitro Study

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12983 - 12983

Published: Dec. 3, 2024

Nonmelanoma skin cancer (NMSC) presents a significant challenge to global healthcare due its rising incidence, prompting the search for innovative treatments overcome limitations of current therapies. Our study aims explore potential effects liquid blood concentrate platelet-rich fibrin (PRF) on basal cell carcinoma cells (BCCs) and squamous (SCCs) in order obtain results that may lead new possible adjunctive therapies managing localized cancers, particularly NMSC. Basal (BCC) (SCC) were indirectly treated with PRF generated via different relative centrifugation forces, namely high low RCF PRF, 7 days. PRF-treated comparatively analyzed viability, proliferation cycle- apoptosis-related gene expression. Analysis MTS assay revealed decrease viability both BCC SCC following treatment Ki-67 staining showed decreased percentage Ki-67-positive after 2 days compared control group. The downregulation CCND1 expression types at along upregulation p21 p53 demonstrated effect inhibiting inducing cycle arrest, especially during initial phases treatment. Increased caspase-8 caspase-9 was observed, indicating activation extrinsic intrinsic apoptotic pathways by Although exact immunomodulatory properties require further investigation, our basic vitro studies are promising might provide basis future investigations as an therapy

Language: Английский

---

In vitro comparison of the effects of titanium-prepared platelet-rich fibrin and leukocyte platelet-rich fibrin on osteoblast behavior and their gen expression

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 26, 2024

To compare the effects of titanium-prepared platelet-rich fibrin (T-PRF) and leukocyte (L-PRF) on osteoblasts. Venous blood samples were collected from ten volunteer patients to obtain T-PRF L-PRF. The group was labelled as Group T, L-PRF L, control group, which includes only osteoblasts, K. PRF added cultured osteoblast cells cell proliferation assessed using an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. effect different groups observed for 72 h results analysed statistically. Additionally, real-time polymerase chain reaction (RT-PCR) conducted evaluate gene expression levels COL1A1, ALP, OCN, RUNX2 within 48 h. We found that at 24 significantly lower than those (p = 0.036 p < 0.001, respectively). showed increase followed by a decrease seen both 24–48 48–72-hour intervals. changes in COLA1, genes did not differ among > 0.05). In this study, we investigated over 72-hour period. Both improved proliferation, however consistent up h, contrast group. No differences found. However, osteoblastic marker can be expressed longer time periods. Therefore, long-term studies are needed.

Language: Английский

---

Evaluating the effectiveness of advanced platelet-rich fibrin, photobiomodulation, pentoxifylline, and Alveogyl in the treatment of alveolar osteitis: a randomized controlled clinical trial

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 26, 2024

Alveolar osteitis is a type of small-scale osteomyelitis the alveolar bone that occurs after tooth extraction, etiology which remains unknown, and alternative methods are being investigated for its treatment. The aim this study was to compare effectiveness advanced platelet-rich fibrin (A-PRF), photobiomodulation (PBM), Alveogyl (butamben, idoform, eugenol), have shown success in treatment osteitis, with pentoxifylline (PTX) determine whether PTX could be an osteitis. This included 80 healthy volunteers diagnosed extraction sockets their mandibular first, second, third molars. patients were divided into four groups, 20 each group: A-PRF, PBM, PTX, Alveogyl. After physiological saline irrigation, treated according respective group. followed up on 2nd, 4th, 7th, 14th days. Primary outcomes pain assessment evaluation soft tissue healing. Pain assessed via visual analog scale (VAS), healing evaluated Landry Healing Index (LHI), granulation measured. Age sex used as variables. data analyzed ANOVA post hoc tests groups. There no statistically significant difference mean VAS score between groups (p > 0.05). However, degree reduction increased following order: In LHI data, there scores favored preoperatively PBM at 2nd week < Statistically differences observed measurements preoperative day, well Days 4 7, favor Day 7 greatest increase A-PRF group, whereas change did not produce healing, although periods when it showed results similar those PBM. score, terms age within time followed. findings indicate has efficacy wound analgesic properties used, does offer advantages. Compared other methods, provided better particularly regarding retrospectively registered clinical trial registry number TCTR20231014003 14.10.2023.

Language: Английский

---