Understanding and measuring mechanical signals in the tumor stroma DOI Creative Commons
Fàtima de la Jara Ortiz,

Chiara Cimmino,

Maurizio Ventre

et al.

FEBS Open Bio, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 10, 2024

The tumor microenvironment (TME) is well known for its immune suppressive role, especially in solid tumors which are characterized by a thick, dense stroma. Apart from cell-cell interactions and biochemical signals, the stroma also distinct mechanical properties, dictated composition architecture of extracellular matrix (ECM). Cancer-associated fibroblasts (CAFs) main producers remodelers stromal ECM, their heterogeneity has recently become focus intense research. This review describes recent findings highlighting CAF subtypes specific functions, as development 3D models to study mechanics vitro. Finally, we discuss quantitative techniques used measure tissue properties at different scales. Given diagnostic prognostic value stiffness composition, anti-tumor therapeutic strategies targeting stroma, understanding measuring never been more timely or relevant.

Language: Английский

Method of Forearm Muscles 3D Modeling Using Robotic Ultrasound Scanning DOI Creative Commons
Vladislava Kapravchuk,

Albert R. Ishkildin,

Andrey Briko

et al.

Sensors, Journal Year: 2025, Volume and Issue: 25(7), P. 2298 - 2298

Published: April 4, 2025

The accurate assessment of muscle morphology and function is crucial for medical diagnostics, rehabilitation, biomechanical research. This study presents a novel methodology constructing volumetric models forearm muscles based on three-dimensional ultrasound imaging integrated with robotic system to ensure precise probe positioning controlled pressure application. proposed scanning approach combined collaborative six-degrees-of-freedom manipulator enabled reproducible high-resolution structures in both relaxed contracted states. A custom-built phantom, acoustically similar biological tissues, was developed validate the method. cross-sectional area coordinates center mass sections, as well volume gravity each muscle, were calculated cross-section reconstructed at contraction. method's feasibility confirmed by comparing volumes anatomical data phantom measurements. highlights advantages robotic-assisted non-invasive suggests its potential applications neuromuscular prosthetics design, rehabilitation monitoring.

Language: Английский

Citations

0
Lipid Microparticle‐Based Phantoms Modeling Hepatic Steatosis for the Validation of Quantitative Imaging Techniques DOI Creative Commons

Connor Endsley,

Shariq Ali, Karim Salhadar

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Abstract Metabolic dysfunction‐associated steatotic liver disease (MASLD) typically presents as “macrovesicular steatosis”, where each hepatocyte contains a large fat vacuole (30‐50 µm), indicating more indolent form. In about 20% of cases, “microvesicular steatosis” occurs, with smaller vacuoles (1‐15 µm) linked to steatohepatitis, cirrhosis progression, and increased risk cancer. Emerging quantitative ultrasound (QUS) quantification (QUS‐LFQ) tools measure various acoustic properties, but few methods compare techniques imaging modalities, the impact size remains unclear. This study introduces methodology create (US) phantoms that replicate vesicle in MASLD. While validate tools, no model currently links QUS‐LFQ measurements steatosis severity. Existing homogeneous assessing properties like attenuation, backscatter, speed sound overlook microstructure steatosis, despite known effect particle on interactions. Here, agar‐based simulate accumulation hepatocytes using stable peanut oil droplets analogs for lipid vacuoles. Microscopy sizing confirm stability at 4 °C, 23 50 °C. Both microscopy US uniform distribution, reflecting content. These hold promise validating methods, particularly US‐based MASLD screening tools.

Language: Английский

Citations

0
Implementation of a Breast Phantom with Acoustic Properties for Ultrasonic Thermometry DOI Creative Commons
Ruth Valeria Acero Mendoza, I. Bazán,

A. Ramírez-García

et al.

Applied Sciences, Journal Year: 2025, Volume and Issue: 15(10), P. 5275 - 5275

Published: May 9, 2025

Breast cancer remains one of the leading causes death among women globally. Early detection is critical for improving patient outcomes, making development accurate and efficient methods essential facilitating timely treatment enhancing patients’ quality life. Lesion sites are often associated with localized temperature increases, which can be identified by characterizing thermal gradients using thermometry tools. Ultrasound-based techniques preferred obtaining patterns due to their noninvasive, non-ionizing nature cost-effectiveness compared like magnetic resonance imaging. This study focuses on developing breast tissue models varying acoustic properties, specifically speed sound across temperatures ranging from 32 °C 36 in increments 0.5 ultrasonic inspection diagnostic applications. These simulate healthy tumorous tissue, including fat, gland, tumor layers. Signal variations were analyzed cross-correlation assess changes as a function temperature. The proposed methodology offers cost-effective, rapid, precise approach phantom production, intervals through analysis acquired signals.

Language: Английский

Citations

0
A Polyvinyl Alcohol (PVA)-Based Phantom for Prostate Cancer Detection Using Multiparametric Ultrasound: A Validation Study DOI Creative Commons

Adel Jawli,

Ghulam Nabi, Zhihong Huang

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(11), P. 1052 - 1052

Published: Oct. 22, 2024

Multiparametric ultrasound (mpUS) enhances prostate cancer (PCa) diagnosis by using multiple imaging modalities. Tissue-mimicking materials (TMM) phantoms, favoured over animal models for ethical and consistency reasons, were created polyvinyl alcohol (PVA) with varying molecular weights (Mw).

Language: Английский

Citations

3
Understanding and measuring mechanical signals in the tumor stroma DOI Creative Commons
Fàtima de la Jara Ortiz,

Chiara Cimmino,

Maurizio Ventre

et al.

FEBS Open Bio, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 10, 2024

The tumor microenvironment (TME) is well known for its immune suppressive role, especially in solid tumors which are characterized by a thick, dense stroma. Apart from cell-cell interactions and biochemical signals, the stroma also distinct mechanical properties, dictated composition architecture of extracellular matrix (ECM). Cancer-associated fibroblasts (CAFs) main producers remodelers stromal ECM, their heterogeneity has recently become focus intense research. This review describes recent findings highlighting CAF subtypes specific functions, as development 3D models to study mechanics vitro. Finally, we discuss quantitative techniques used measure tissue properties at different scales. Given diagnostic prognostic value stiffness composition, anti-tumor therapeutic strategies targeting stroma, understanding measuring never been more timely or relevant.

Language: Английский

Citations

0