Exploring the promising potential of induced pluripotent stem cells in cancer research and therapy

Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)

Published: Nov. 28, 2023

The advent of iPSCs has brought about a significant transformation in stem cell research, opening up promising avenues for advancing cancer treatment. formation is multifaceted process influenced by genetic, epigenetic, and environmental factors. offer distinctive platform investigating the origin cancer, paving way novel approaches to treatment, drug testing, tailored medical interventions. This review article will provide an overview science behind iPSCs, current limitations challenges iPSC-based therapy, ethical social implications, comparative analysis with other types also discuss applications tumorigenesis, future tumorigenesis highlight successful case studies utilizing research. conclusion summarize advancements made research importance continued investment iPSC unlock full potential these cells.

Language: Английский

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Organs-on-chips technologies – A guide from disease models to opportunities for drug development

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 231, P. 115271 - 115271

Published: March 31, 2023

Current in-vitro 2D cultures and animal models present severe limitations in recapitulating human physiopathology with striking discrepancies estimating drug efficacy side effects when compared to trials. For these reasons, microphysiological systems, organ-on-chip multiorgans microdevices attracted considerable attention as novel tools for high-throughput high-content research achieve an improved understanding of diseases accelerate the development process towards more precise eventually personalized standards. This review takes form a guide on this fast-growing field, providing useful introduction major themes indications further readings. We start analyzing Organs-on-chips (OOC) technologies testing administration routes: (1) oral/rectal route by intestine-on-a-chip, (2) inhalation lung-on-a-chip, (3) transdermal skin-on-a-chip (4) intravenous through vascularization models, considering how drugs penetrate bloodstream are conveyed their targets. Then, we focus OOC (other) specific organs diseases: neurodegenerative brain blood barriers, tumor including vascularization, organoids/spheroids, engineering screening antitumor drugs, liver/kidney chips multiorgan gastrointestinal metabolic assessment biomechanical systems heart, muscles bones structures related diseases. Successively, discuss materials organ chips, microfluidic organs-on-chips, sensor integration real-time monitoring, cell lines chips. (Nano)delivery approaches therapeutics chip also described. Finally, conclude critical discussion current significance/relevance, trends, limitations, challenges future prospects terms revolutionary impact biomedical research, preclinical development.

Language: Английский

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Recent advances for cancer detection and treatment by microfluidic technology, review and update

Biological Procedures Online, Journal Year: 2022, Volume and Issue: 24(1)

Published: April 28, 2022

Abstract Numerous cancer-associated deaths are owing to a lack of effective diagnostic and therapeutic approaches. Microfluidic systems for analyzing low volume samples offer precise, quick, user-friendly technique cancer diagnosis treatment. devices can detect many cancer-diagnostic factors from biological fluids also generate appropriate nanoparticles drug delivery. Thus, microfluidics may be valuable in the field due its high sensitivity, throughput, cost. In present article, we aim review recent achievements application microfluidic treatment various cancers. Although platforms not yet used clinic, they expected become main technology proving more sensitive accurate detection biomarkers strategies than common assays. lab-on-a-chip have shown remarkable potential designing novel procedures detection, therapy, disease follow-up as well development new delivery

Language: Английский

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3D cancer models: One step closer to in vitro human studies

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: April 11, 2023

Cancer immunotherapy is the great breakthrough in cancer treatment as it displayed prolonged progression-free survival over conventional therapies, yet, to date, only a minority of patients. In order broad clinical applicability some roadblocks need be overcome, first among all lack preclinical models that faithfully depict local tumor microenvironment (TME), which known dramatically affect disease onset, progression and response therapy. this review, we provide reader with detailed overview current 3D developed mimick complexity dynamics TME, focus on understanding why TME major target anticancer We highlight advantages translational potentials spheroids, organoids immune Tumor-on-a-Chip modeling therapeutic response, while outlining pending challenges limitations. Thinking forward, possibility integrate know-hows micro-engineers, immunologists, pharmaceutical researchers bioinformaticians meet needs clinicians interested using these platforms high fidelity for patient-tailored drug discovery.

Language: Английский

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3D bioprinting complex models of cancer

Biomaterials Science, Journal Year: 2023, Volume and Issue: 11(10), P. 3414 - 3430

Published: Jan. 1, 2023

Cancer is characterized by the uncontrolled division of cells, resulting in formation tumors. The personalized composition biomaterials and 3D techniques can be used as a modelling tool to understand complexity various cancers.

Language: Английский

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Microfluidic systems for modeling digestive cancer: a review of recent progress

Biomedical Physics & Engineering Express, Journal Year: 2024, Volume and Issue: 10(5), P. 052002 - 052002

Published: Aug. 14, 2024

Abstract Purpose . This review aims to highlight current improvements in microfluidic devices designed for digestive cancer simulation. The emphasizes the use of multicellular 3D tissue engineering models understand complicated biology tumor microenvironment (TME) and progression. purpose is develop oncology research improve patients’ lives. Methods analyzes recent on mimicking cancer. It uses tissue-engineered devices, notably organs a chip (OOC), simulate human organ function lab. Cell cultivation modern three-dimensional hydrogel platforms allows precise geometry, biological components, physiological qualities. novel methodologies, key findings, technical progress explain this field’s advances. Results study discusses advances Micro systems with are emphasized. These capture complex biochemical gradients, niche variables, dynamic cell–cell interactions (TME). reveal stomach progression by duplicating TME. Recent discoveries technology have improved our understanding gut biology, as shown review. Conclusion Microfluidic play crucial role modeling furthering research. could transform drug development treatment revealing provides complete summary suggests future field professionals. review’s major goal further medical

Language: Английский

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Recent advances in 3D-printing-based organ-on-a-chip

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1), P. 100003 - 100003

Published: March 21, 2024

Organ-on-a-chip (OOC) facilitates precise manipulation of fluids in microfluidic chips and simulation the physiological, chemical, mechanical characteristics tissues, thus providing a promising tool for vitro drug screening physiological modeling. In recent decades, this technology has advanced rapidly because development various three-dimensional (3D) printing techniques. 3D can not only fabricate using materials such as resins polydimethylsiloxane but also construct biomimetic tissues bioinks cell-loaded hydrogels. review, advances 3D-printing-based OOC are systematically summarized based on used direct or indirect OOC, techniques construction applications models heart, blood vessels, intestines, liver, kidney. addition, future perspectives challenges area envisioned to inspire researchers employ accelerate development.

Language: Английский

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Cancer Metastasis‐on‐a‐Chip for Modeling Metastatic Cascade and Drug Screening

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(21)

Published: Jan. 15, 2024

Microfluidic chips are valuable tools for studying intricate cellular and cell-microenvironment interactions. Traditional in vitro cancer models lack accuracy mimicking the complexities of vivo tumor microenvironment. However, cancer-metastasis-on-a-chip (CMoC) combine advantages 3D cultures microfluidic technology, serving as powerful platforms exploring mechanisms facilitating drug screening. These able to compartmentalize metastatic cascade, deepening understanding its underlying mechanisms. This article provides an overview current CMoC models, focusing on distinctive that simulate invasion, intravasation, circulation, extravasation, colonization, their applications Furthermore, challenges faced by technologies discussed, while promising future directions research. The ongoing development integration these into studies expected drive transformative advancements field.

Language: Английский

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Advances, challenges and future applications of liver organoids in experimental regenerative medicine

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 24, 2025

The liver is a vital organ responsible for numerous metabolic processes in the human body, including metabolism of drugs and nutrients. After damage, can rapidly return to its original size if causative factor promptly eliminated. However, when harmful stimulus persists, liver’s regenerative capacity becomes compromised. Substantial theoretical feasibility has been demonstrated at levels gene expression, molecular interactions, intercellular dynamics, complemented by successful animal studies. robust model carrier that closely resemble physiology are still lacking translating these theories into practice. potential regeneration central focus ongoing research. Over past decade, advent organoid technology provided improved models materials advancing research efforts. Liver represents novel vitro culture system. several years refinement, organoids now accurately replicate morphological structure, nutrient drug metabolism, secretory functions, providing disease Regenerative medicine aims or tissue functions repair replace damaged tissues, restore their structure function, stimulate tissues organs within body. possess same function as tissue, offering serve viable replacement liver, aligning with goals medicine. This review examines role

Language: Английский

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The Variety of 3D Breast Cancer Models for the Study of Tumor Physiology and Drug Screening

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7116 - 7116

Published: April 12, 2023

Breast cancer is the most common in women and responsible for multiple deaths worldwide. 3D models enable a better representation of tumor physiology than conventional 2D cultures. This review summarizes important components physiologically relevant describes spectrum breast models, e.g., spheroids, organoids, on chip bioprinted tissues. The generation spheroids relatively standardized easy to perform. Microfluidic systems allow control over environment inclusion sensors can be combined with or models. strength bioprinting relies spatial cells modulation extracellular matrix. Except predominant use cell lines, differ stromal composition, matrices fluid flow. Organoids are appropriate personalized treatment, but all technologies mimic aspects physiology. Fetal bovine serum as culture supplement Matrigel scaffold limit reproducibility standardization listed integration adipocytes needed because they possess an role cancer.

Language: Английский

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