Advancing environmental sustainability through microbial reprogramming in growth improvement, stress alleviation, and phytoremediation

Plant Stress, Journal Year: 2023, Volume and Issue: 10, P. 100283 - 100283

Published: Nov. 7, 2023

The substantial influence of microbes on crop growth, stress resilience, and ecological restoration has generated considerable interest due to the intricate interplay between these microorganisms plants. This study comprehensively examines diverse mechanisms through which contribute plant well-being, mitigate stress, facilitate phytoremediation processes. Microorganisms encompassing bacteria, fungi, archaea, viruses, have demonstrated their knack for stirring up growth-enabling hormones, activating pathways tuned ameliorating availability nutrients by means fixation solubilization Furthermore, such also display immense potential in field strategies aiding plants extraction, alteration, detoxification contaminants found both soil water. Complementing this, enable phytoextraction, rhizofiltration, phytostabilization, rhizodegradation, owing harmonious interaction with purification tainted environments. However, it is critical address legal issues, moral dilemmas, unintended consequences as are increasingly incorporated into sustainable agriculture methods. Optimizing microbial therapies ensuring appropriate use offers promising insights when leveraging cutting-edge technologies like omics genetic engineering. Coordination among academics, practitioners, policymakers still crucial quest a more robust peaceful coexistence microbes, plants, ecosystems. In nutshell, this work highlights pivotal role that poised assume, guiding trajectory agriculture, alleviating fostering environmental sustainability far-reaching implications.

Language: Английский

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Chalcone synthase (CHS) family genes regulate the growth and response of cucumber (Cucumis sativus L.) to Botrytis cinerea and abiotic stresses

Plant Stress, Journal Year: 2023, Volume and Issue: 8, P. 100159 - 100159

Published: April 24, 2023

The chalcone synthase (CHS) gene family is instrumental not only regulating plant growth and development but fine-tuning response to environmental hazards. Despite the growing body of literature, role CHS genes in cucumber (Cucumis sativus L.) remains elusive. Herein, we identified 4 CsCHS from genome database. evolutionary relationship, structure, conserved motifs, proteins interaction chemical properties highlight key aspects cucumbers. Evolutionary tree analysis reveals that divided into four clades. Moreover, Gene Ontology (GO) showed regulate various stimuli. Microarray expression data were expressed almost all tested tissues. Additionally, differential patterns found under salinity stress hormonal treatments. qRT-PCR aphid infestation revealed they are components defense against insect pests. Under waterlogging stress, CsCHS3 displayed high naphthalene acetic acid (NAA) ethylene (ETH). dual luciferase assay indicated CsCHS2 effectively binds promoters markers CsERF1 CsERF3. Our results have provided detailed knowledge regarding gene's influential also providing resistive plants. We believe our study will facilitate breeding stress-resilient cultivars, particularly pests stress.

Language: Английский

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TALE gene family: identification, evolutionary and expression analysis under various exogenous hormones and waterlogging stress in Cucumis sativus L.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: June 15, 2024

Abstract Background Three Amino acid Loop Extension (TALE) belongs to the homeobox group of genes that are important constituents plant systems. The TALE gene family is instrumental not only in growth and development but also plays an essential role regulating response environmental adversaries. Results In present study, we isolated 21 CsTALE from cucumber ( Cucumis sativus L.) genome database. Bioinformatics tools were put place understand structural functional components family. evolutionary analysis dissected them into seven subclades (KNOX-I, KNOX-II, BELL-I BELL-V). c - acting elements promoter region disclosed they key regulators hormonal stress-related processes. Additionally, STRING database advocated concerting proteins with other transcription factors potent developmental biology. CsmiR319 CsmiR167a-3p targeting CsTALE15 CsTALE16 , respectively, further assert importance posttranscriptional-related Tissue-specific expression unfolded fundamental involvement as expressed throughout stages. Under waterlogging stress, CsTALE17 significantly higher values WL, WL-NAA, WL-ETH WL-MeJA-treated samples. Conclusions study reveals evolution functions cucumber. Our work will provide a platform help future researchers address issue stress Yangtze River Delta.

Language: Английский

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GmNAC3 acts as a key regulator in soybean against drought stress

Current Plant Biology, Journal Year: 2024, Volume and Issue: 38, P. 100346 - 100346

Published: May 3, 2024

The NAC (NAM, ATAF and CUC) family is one of the largest transcription factor (TF) families in plant that are involved regulatory mechanisms growth development as well responses to abiotic stresses. However, underlying molecular mechanism drought-responsive members soybean still remains inexplicit. In this study, a total 179 GmNAC genes were identified genome. We discovered majority have more than three exons share gene motif structure mostly conserved at N-terminus. Phylogenetic analysis suggested proteins divided into 10 separate groups. cis-elements highlighted potential role various hormonal defense related activities. addition, most showed notable expression roots leaves, suggesting their likely stress adaptation. overexpression GmNAC3-OE Arabidopsis increased tolerance drought stress. Similarly, plants displayed better survival rates, root length antioxidant Enhanced specific was also recorded. Our findings revealed GmNAC3 regulating response could be used marker generate resilient plants.

Language: Английский

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Genome-wide identification, characterization, and expression analysis of the Chalcone Synthase gene family in Oryza sativa under Abiotic Stresses

Plant Stress, Journal Year: 2023, Volume and Issue: 9, P. 100201 - 100201

Published: Aug. 9, 2023

Chalcone synthase (CHS) is the pivotal enzyme that catalyzes first committed step of phenylpropanoid pathway leading to flavonoids. Herein, we identified twenty-seven OsCHS genes from rice genome database. The bioinformatics analysis, including conserved motif, domain, interactive proteins, gene ontology (GO), target micro RNAs (miRNA) prediction, and physiochemical properties, highlight key aspects in Oryza sativa L. proteins' phylogeny was further clustered into 4 sub-groups (Group I- Group IV) based on structural similarities functional diversities. GO analysis proteins showed they are highly stress-responsive (biotic/abiotic) involved various growth developmental processes. microarray expression transcription twelve tissues, differential pattern revealed their possible involvement against salinity drought. higher OsCHS15 OsWRKY65-OE line indicates its potential hormonal-mediated stress response. predicted miRNA includes miR2260, miR167, miR11337, miR2232families engagement posttranslational regulation stress-responsive. Owing above-mentioned outcomes, speculate O. play an essential role not only development but also fine-tuning immune responses environmental stresses provide major indications for studies.

Language: Английский

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microRNA regulates cytokinin induced parthenocarpy in cucumber (Cucumis sativus L.)

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 212, P. 108681 - 108681

Published: May 13, 2024

Language: Английский

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Transcriptomic analysis reveals the crucial role of YABBY genes family in hormonal induced parthenocarpy in Cucumis sativus L.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 11, 2025

The plant-specific YABBY transcription factor family plays several activities, including responding to abiotic stress, establishing dorsoventral polarity, and developing lateral organs. Cucumis sativus L. commonly referred as cucumber one of the first vegetable crops with a fully sequenced genome. In this work, we examined application NAA, CPPU, GA4 + 7 inflict parthenocarpy in ZK line. expression pattern genes throughout fruit development performed genomic census (Cucumis L.). Based on peptide classification, discovered eight CsYABBY divided them into five subfamilies. Similarities motif composition exon-intron structure were also observed. cis-elements gene ontology (GO) analysis revealed involvement vegetative growth transition reproductive phase. differential response 7. particular, CsYABBY1 was induced sharply by NAA CPPU but not transient CsCRC disclosed that it is localized nucleus. These findings point possibility may positively affect could be utilized generate parthenocarpic fruits.

Language: Английский

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Methyl Jasmonate Alleviates the Deleterious Effects of Salinity Stress by Augmenting Antioxidant Enzyme Activity and Ion Homeostasis in Rice (Oryza sativa L.)

Agronomy, Journal Year: 2022, Volume and Issue: 12(10), P. 2343 - 2343

Published: Sept. 28, 2022

Methyl jasmonate (MeJA) is a potent player that fine-tunes growth and developmental activities under salinity stress. In this study, we investigated the influence of MeJA on two rice cultivars (NJ9108 XD22) subjected to different stresses. Following stress treatment, reduction in water use efficiency, relative contents, membrane stability index both were observed, whereas treatment partially alleviated negative effects. significantly increased maximum photochemical efficiency (Fv/Fm) electron transfer photosystem II (Fv/Fo). Under stress, triggered H2O2 APX accumulation, while POD SOD remained unchanged cultivars. Salt Na+ concentration roots leaves but decreased K+ K+/Na+ ratio However, MeJA-treated plants had accumulation saline conditions. The differential expression pattern OsHKT OsHAK genes implied ion homeostasis crucial salt These findings suggest application can be an alternative source reducing without compromising yield.

Language: Английский

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Identification and Molecular Characterization of RWP-RK Transcription Factors in Soybean

Genes, Journal Year: 2023, Volume and Issue: 14(2), P. 369 - 369

Published: Jan. 31, 2023

The RWP-RK is a small family of plant-specific transcription factors that are mainly involved in nitrate starvation responses, gametogenesis, and root nodulation. To date, the molecular mechanisms underpinning nitrate-regulated gene expression many plant species have been extensively studied. However, regulation nodulation-specific NIN proteins during nodulation rhizobial infection under nitrogen soybean still remain unclear. Here, we investigated genome-wide identification their essential role nitrate-inducible stress-responsive soybean. In total, 28 genes were identified from genome, which unevenly distributed on 20 chromosomes 5 distinct groups phylogeny classification. conserved topology protein motifs, cis-acting elements, functional annotation has led to potential as key regulators growth, development, diverse stress responses. RNA-seq data revealed up-regulation GmRWP-RK nodules indicated these might play crucial roles Furthermore, qRT-PCR analysis most Phytophthora sojae environmental conditions (such heat, nitrogen, salt) significantly induced, thus opening new window possibilities into regulatory adaptation allow tolerate biotic abiotic stress. addition, dual luciferase assay GmRWP-RK1 GmRWP-RK2 efficiently bind promoters GmYUC2, GmSPL9, GmNIN, highlighting possible involvement nodule formation. Together, our findings provide novel insights defense responses

Language: Английский

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Inherited endurance: deciphering genetic associations of transgenerational and intergenerational heat stress memory in barley

Plant Molecular Biology, Journal Year: 2025, Volume and Issue: 115(2)

Published: March 10, 2025

Language: Английский

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