Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep

Animal Reproduction Science, Journal Year: 2021, Volume and Issue: 246, P. 106904 - 106904

Published: Dec. 3, 2021

Sperm cryopreservation is one of the most important procedures in development biotechnologies for assisted reproduction. In some farm animals, use cryopreserved sperm has so many benefits which relevance become more evident recent decades. Values post-thaw quality, however, are variable among species and within individuals same species. There no standardized methodology each stages procedure (andrological examination, semen collection, dilution, centrifugation, resuspension pellet with freezing medium, packaging, evaluation), also contributes to differences studies. Cryotolerance markers seminal plasma (SP) have been evaluated prediction ejaculate freezability. addition, previous research, there a focus on supplementing media different substances, such as enzymatic non-enzymatic antioxidants. studies, inclusion these substances led improved quality fertilizing capacity result minimizing adverse effects structure function. Another approach cryoprotectants. The aim this review article provide an update animals. main detrimental described, including negative repercussion reproductive performance. Furthermore, potential molecular biomarkers predict cryotolerance discussed, well addition that can mitigate harmful impact thawing sperm.

Language: Английский

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Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly

Biology of Reproduction, Journal Year: 2024, Volume and Issue: 110(6), P. 1135 - 1156

Published: April 19, 2024

Abstract Conventional, brightfield-microscopic semen analysis provides important baseline information about sperm quality of an individual; however, it falls short identifying subtle subcellular and molecular defects in cohorts “bad,” defective human animal spermatozoa with seemingly normal phenotypes. To bridge this gap, is desirable to increase the precision andrological evaluation humans livestock animals by pursuing advanced biomarker-based imaging methods. This review, spiced up occasional classic movie references but seriously scholastic at same time, focuses mainly on biomarkers altered male germ cell proteostasis resulting post-testicular carryovers proteins associated ubiquitin-proteasome system. Also addressed are redox homeostasis, epididymal maturation, sperm–seminal plasma interactions, surface glycosylation. Zinc ion homeostasis-associated sperm-borne components, including elements neurodegenerative pathways such as Huntington Alzheimer disease, discussed. Such spectrum biomarkers, imaged highly specific vital fluorescent probes, lectins, antibodies, reveals both obvious chromatin, deoxyribonucleic acid, accessory structures head tail. Introduction next-generation image-based flow cytometry into research clinical andrology will soon enable incorporation machine deep learning algorithms end point developing simple, label-free methods for diagnostics high-throughput phenotyping economically animals.

Language: Английский

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Factors affecting the success of laparoscopic artificial insemination in sheep

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 264, P. 107453 - 107453

Published: March 14, 2024

Successful artificial breeding underpins rapid genetic and production gains in animal agriculture. In sheep, insemination with frozen semen is performed via intrauterine laparoscopy as frozen-thawed spermatozoa do not traverse the cervix sufficient numbers for high fertility transcervical anatomically impossible most ewes. Historically, laparoscopic has always been considered reasonably successful, but recent anecdotal reports of poor place it at risk warning adoption. Understanding male, female environmental factors that influence sheep warranted if success to be improved progress maximised industry. This review details current practice AI sheep. It explores effects quantity quality, ewe, her preparation, conditions, on obtained following insemination.

Language: Английский

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ProAKAP4 Semen Concentrations as a Valuable Marker Protein of Post-Thawed Semen Quality and Bull Fertility: A Retrospective Study

Veterinary Sciences, Journal Year: 2022, Volume and Issue: 9(5), P. 224 - 224

Published: May 6, 2022

Functional sperm quality markers to predict bull fertility have been actively investigated. Among them, proAKAP4, which is the precursor of AKAP4, main structural protein in fibrous sheath spermatozoa; appears be promising, especially since spermatozoa lacking AKAP4 expression were shown immotile, abnormal, and infertile. In this study, objective was evaluate proAKAP4 concentration values with classic motility descriptors outcomes (NRR at 90 days) post-thawed conditions 10 bulls’ semen. ProAKAP4 confirmed by Western blotting concentrations determined ELISA. Variations observed independently measured using computer-assisted semen analysis (CASA). A 38.67 ± 8.55 ng/10 million obtained as a statistical mean all samples. Threshold then between 19.96 96.95 spermatozoa. positively correlated progressive linearity coefficient. The showing lowest samples exhibiting below 20 Furthermore, significantly higher bulls NRR field. Our results demonstrate correlation NRR-90d (p = 0.05) semen, highlighting potential predictive marker fertility.

Language: Английский

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Multiparametric Study of Antioxidant Effect on Ram Sperm Cryopreservation—From Field Trials to Research Bench

Animals, Journal Year: 2021, Volume and Issue: 11(2), P. 283 - 283

Published: Jan. 23, 2021

The optimization of sperm cryopreservation protocols in ram is a feasible tool to reinforce artificial insemination technologies considering the desirable application by vaginal/cervical or transcervical deposition. Cryopreservation provokes different types damage on spermatozoa and many these detrimental effects are triggered redox deregulation. For this reason, antioxidant supplementation decrease reactive oxygen species (ROS) levels equilibrate status has been widely employed species. Despite this, more fertility trials necessary provide definitive ensure effectiveness quality. work, we performed multiparametric analysis some previously tested antioxidants (crocin, GSH Trolox) from field quality analyses focused new strategies measure balance. Attending trial, Trolox registered an improvement concerning (when considered high males) multiple lambing frequency other complementary descriptive data related performance such as prolificacy fecundity. This positive effect was evident when low males than global male analysis. In vitro confirmed vivo registering viability study, provided evidence that role trolox balance maintenance direct parameters, prolificacy. treatments tested, for first time ovine species, using integrative approach combining novel approaches, RedoxSYS. These should be applied improve conservation methods optimize AI upgrading correlation between analyses.

Language: Английский

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ProAKAP4 as a motility long-lasting marker in Catalan donkey spermatozoa

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 262, P. 107427 - 107427

Published: Feb. 7, 2024

ProAKAP4 is identified within the flagellum of spermatozoa in various mammalian species, serving as a structural protein associated with motility parameters. This investigation focuses on presence proAKAP4 donkey sperm, elucidating its localization, molecular characteristics, and correlation descriptors mitochondrial membrane potential. Twelve ejaculates from Catalan donkeys were analyzed this study. The initial steps involved sequencing detection through Western blotting immunofluorescence. Post-thaw assessments conducted at 0, 1, 3 hours, encompassing levels, sperm via Computer-Assisted Sperm Analysis (CASA), potential determined by flow cytometry using JC-1 stain. findings reveal that exhibits characteristic localization principal piece flagellum, consistent observations other mammals. weight to be 100 kDa. Significantly, positive (p≤0.05) established between concentration both total progressive motility. cryoprotectant lower concentration. Notably, experiences substantial decrease during hour post-thawing. In conclusion, akin It motility, notably affected significant consumption observed following thawing. These contribute our understanding dynamics providing insights may have implications for semen preservation reproductive technologies equids.

Language: Английский

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An updated review on the application of proteomics to explore sperm cryoinjury mechanisms in livestock animals

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 263, P. 107441 - 107441

Published: Feb. 21, 2024

Language: Английский

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ProAKAP4 as Indicator of Post-thaw Motility in Stallion

Published: March 25, 2024

ProAKAP4, a precursor of AKAP4 (A-kinase anchor protein) found in the flagellum mammalian and non-mammalian spermatozoa, serves as structural protein with established correlations to motility parameters across diverse species. This study aimed determine proAKAP4 levels evolution thawed stallion semen over 3-hour period, examining its correlation descriptors mitochondrial membrane potential. Utilizing sixteen ejaculates from four French warmblood stallions, involved maintaining samples at 37°C for 3 hours, conducting enzyme-linked immunosorbent assay (ELISA), computer-assisted sperm analysis (CASA), potential by JC-1 probe flow cytometry 0, 1, hours post-thawing. The findings indicate significant positive (p≤0.05) between total or progressive all time points analyzed. Spermatozoa velocity (VAP, VCL, VSL) spermatozoa lateral head displacement (ALH) display ProAKAP4 0-hour concentration exhibits no discernible difference batches without cryoprotectant. Notably, consumption remains insignificant within initial hour after thawing but becomes 1 In summary, demonstrates up thawing, albeit showing noticeable decrease starting first post-thawing, indicating result motile activity.

Language: Английский

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Deciphering the mechanism of sperm cell aging - functional, structural, and kinematic characteristics in fresh semen of mature and aged dogs

Animal Reproduction Science, Journal Year: 2025, Volume and Issue: 274, P. 107798 - 107798

Published: Feb. 12, 2025

Language: Английский

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A‐Kinase Anchor Protein 4 (proAKAP4): Protein Molecule–Based Fertility Marker of Indonesian Dairy Bull and Its Correlation With Frozen‐Thawed Sperm Quality

Veterinary Medicine International, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

The precursor protein of A-kinase anchor 4 (proAKAP4), which is abundant in the fibrous sheath sperm, plays an essential role sperm motility and has been developed into one candidate molecular-based fertility markers. This study aimed to identify proAKAP4 Indonesian dairy bulls investigate its correlation with various quality characteristics. findings are expected be applied optimize use superior bulls. Frozen semen from 20 were used, had previously classified based on their levels two groups: high (HF) low (LF). Analysis frozen-thawed quality, such as progressive total motility, kinematics, membrane integrity, mitochondrial potential, acrosome capacitation status, was carried out both groups. ProAKAP4 analyzed using Bull 4MID Kit ELISA technique. Each bull showed varying abundance (p < 0.05) another. Frozen-thawed HF higher than LF bulls, especially noncapacitated sperm. parameters capacitated acrosome-reacted opposite results, where results identification that Correlation analysis confirmed a strong association between rate. great potential marker for determining selecting

Language: Английский

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