Reviewer #1 (Public Review): SERBP1 interacts with PARP1 and is present in PARylation-dependent protein complexes regulating splicing, cell division, and ribosome biogenesis DOI Open Access

Published: July 2, 2024

RNA binding proteins (RBPs) containing intrinsically disordered regions (IDRs) are present in diverse molecular complexes where they function as dynamic regulators. Their characteristics promote liquid-liquid phase separation (LLPS) and the formation of membraneless organelles such stress granules nucleoli. IDR-RBPs particularly relevant nervous system their dysfunction is associated with neurodegenerative diseases brain tumor development. SERBP1 a unique member this group, being mostly lacking canonical RNA-binding domains. Using proteomics approach followed by functional analysis, we defined SERBP1's interactome. We uncovered novel roles splicing, cell division, ribosomal biogenesis showed its participation pathological Tau aggregates Alzheimer's disease brains. preferentially interacts other G-quadruplex (G4) binders, implicated different stages gene expression, suggesting that G4 critical component settings. Similarly, identified important associations between PARP1/polyADP-ribosylation (PARylation). PARP1 factors influences PARylation. Moreover, protein which participates contain PARylated PAR binders. Based on these results, propose feedback regulatory model PARylation, while PARylation modulates functions complexes.

Language: Английский

PARP1 at the crossroad of cellular senescence and nucleolar processes DOI

Kinga Kołacz,

Agnieszka Robaszkiewicz

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 94, P. 102206 - 102206

Published: Jan. 24, 2024

Language: Английский

Citations

8
Drug addiction and treatment: An epigenetic perspective DOI Open Access
Arunkumar Singh Koijam,

Kabrambam Dasanta Singh,

Bunindro Nameirakpam

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 170, P. 115951 - 115951

Published: Dec. 3, 2023

Drug addiction is a complex disease affected by numerous genetic and environmental factors. Brain regions in reward pathway, neuronal adaptations, epigenetic interactions causing transcriptional enhancement or repression of multiple genes induce different phenotypes for varying duration. Addictive drug use causes alterations similarly changes induced environment can promote addiction. Epigenetic mechanisms include DNA methylation post-translational modifications like methylation, acetylation, phosphorylation, ubiquitylation, sumoylation, dopaminylation crotonylation histones, ADP-ribosylation. Non-coding RNAs also changes. This review discusses these above areas stresses the need exploring epidrugs as treatment alternative adjunct, considering limited success current strategies. Epigenome editing complexes have lately been effective eukaryotic systems. Targeted cleavage techniques such CRISPR-Cas9 system, CRISPR-dCas9 complexes, transcription activator-like effector nucleases (TALENs) zinc-finger (ZFNs) exploited targeted recognition anchoring platforms, fused with writer eraser proteins delivered transfection transduction methods. Efficacy seen various neuropsychiatric conditions initial results involving model organisms are remarkable. Epidrugs present promising

Language: Английский

Citations

11
SERBP1 interacts with PARP1 and is present in PARylation-dependent protein complexes regulating splicing, cell division, and ribosome biogenesis DOI Open Access

Kira Breunig,

Xiufen Lei,

Mauro Montalbano

et al.

Published: Jan. 6, 2025

RNA binding proteins (RBPs) containing intrinsically disordered regions (IDRs) are present in diverse molecular complexes where they function as dynamic regulators. Their characteristics promote liquid-liquid phase separation (LLPS) and the formation of membraneless organelles such stress granules nucleoli. IDR-RBPs particularly relevant nervous system their dysfunction is associated with neurodegenerative diseases brain tumor development. Serpine1 mRNA-binding protein 1 (SERBP1) a unique member this group, being mostly lacking canonical RNA-binding domains. We defined SERBP1’s interactome, uncovered novel roles splicing, cell division ribosomal biogenesis, showed its participation pathological Tau aggregates Alzheimer’s brains. SERBP1 preferentially interacts other G-quadruplex (G4) binders, implicated different stages gene expression, suggesting that G4 critical component settings. Similarly, we identified important associations between PARP1/polyADP-ribosylation (PARylation). PARP1 factors influences PARylation. Moreover, which participates contain PARylated PAR binders. Based on these results, propose feedback regulatory model PARylation, while PARylation modulates functions complexes.

Language: Английский

Citations

0
SERBP1 interacts with PARP1 and is present in PARylation-dependent protein complexes regulating splicing, cell division, and ribosome biogenesis DOI Creative Commons

Kira Breunig,

X. Lei,

Mauro Montalbano

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 12, 2025

RNA binding proteins (RBPs) containing intrinsically disordered regions (IDRs) are present in diverse molecular complexes where they function as dynamic regulators. Their characteristics promote liquid-liquid phase separation (LLPS) and the formation of membraneless organelles such stress granules nucleoli. IDR-RBPs particularly relevant nervous system their dysfunction is associated with neurodegenerative diseases brain tumor development. Serpine1 mRNA-binding protein 1 (SERBP1) a unique member this group, being mostly lacking canonical RNA-binding domains. We defined SERBP1’s interactome, uncovered novel roles splicing, cell division ribosomal biogenesis, showed its participation pathological Tau aggregates Alzheimer’s brains. SERBP1 preferentially interacts other G-quadruplex (G4) binders, implicated different stages gene expression, suggesting that G4 critical component settings. Similarly, we identified important associations between PARP1/polyADP-ribosylation (PARylation). PARP1 factors influences PARylation. Moreover, which participates contain PARylated PAR binders. Based on these results, propose feedback regulatory model PARylation, while PARylation modulates functions complexes.

Language: Английский

Citations

0
Development of Ferroptosis-Associated ceRNA Network in Periodontitis DOI Creative Commons
Churen Zhang,

Pengxin Xue,

Jianguo Ke

et al.

International Dental Journal, Journal Year: 2022, Volume and Issue: 73(2), P. 186 - 194

Published: July 7, 2022

Periodontitis is a chronic inflammatory illness that may lead to tooth loosening and even loss, its pathogenesis not fully understood. Ferroptosis an iron-dependent, regulated cell death. The present study aims find the key ferroptosis-related genes (FRGs) in periodontitis develop mRNA-miRNA-lncRNA network deeply explore of periodontitis.Data from Gene Expression Omnibus (GEO) database FerrDb were downloaded discover differentially expressed mRNA, miRNA, FRGs. Functional enrichment analysis was conducted for FRGs (DE-FRGs), including gene ontology, Kyoto Encyclopedia Genes Genomes (KEGG) pathway, protein-protein interaction (PPI) analysis. Targetscan miRtarbase used estimate miRNAs DE-FRGs interact with, whilst StarBase v3.0 lncRNA-miRNA interaction.Seven identified through differential expression Interleukin 1 beta (IL1B) interacted with XBP1 MMP13 PPI network. After taking intersection between DE-miRNAs predicted miRNAs, ceRNA containing IL1B, has-miR-185, has-miR-204, has-miR-211, has-miR-4306, 28 lncRNAs established.Seven identified, which might promote deeper understanding ferroptosis periodontitis.

Language: Английский

Citations

15
The DarT/DarG Toxin–Antitoxin ADP-Ribosylation System as a Novel Target for a Rational Design of Innovative Antimicrobial Strategies DOI Creative Commons
Giuliana Catara, Rocco Caggiano, Luca Palazzo

et al.

Pathogens, Journal Year: 2023, Volume and Issue: 12(2), P. 240 - 240

Published: Feb. 2, 2023

The chemical modification of cellular macromolecules by the transfer ADP-ribose unit(s), known as ADP-ribosylation, is an ancient homeostatic and stress response control system. Highly conserved across evolution, ADP-ribosyltransferases ADP-ribosylhydrolases ADP-ribosylation signalling responses. In addition to proteins, both prokaryotic eukaryotic transferases can covalently link different conformations nucleic acids, thus highlighting evolutionary conservation archaic mechanisms. Here, we report several structural functional aspects DNA controlled prototype DarT DarG pair, which show ADP-ribosyltransferase hydrolase activity, respectively. DarT/DarG a toxin-antitoxin system in many bacterial pathogens, for example Mycobacterium tuberculosis, regulates two clinically important processes human health, namely, growth anti-phage response. modulation selective inhibitors may represent exciting strategy tackle resistance current antimicrobial therapies.

Language: Английский

Citations

6
Roles of DNA damage repair and precise targeted therapy in renal cancer (Review) DOI Creative Commons
Yongchang Lai, Zhibiao Li, Zechao Lu

et al.

Oncology Reports, Journal Year: 2022, Volume and Issue: 48(6)

Published: Oct. 19, 2022

The primary subtypes of renal cell carcinoma (RCC) include clear cell, papillary and chromophobe RCC. RCC occurs often due to loss von Hippel‑Lindau (VHL) accumulation lipids glycogen, cells may exhibit sensitivity the disruption normal metabolism or homologous recombination gene defect. Although application molecular‑targeted drugs (tyrosine kinase inhibitors) immune checkpoint inhibitors has been recommended for treatment advanced RCC, more targets DNA damage repair (DDR) signaling pathway involved in synthetic lethal effect have investigated. However, although achievements made exploration roles DDR genes on progression, their association not systematically summarized. Poly (ADP‑ribose) polymerase (PARP) 1 are used tumors with BRCA1/2 repair‑associated mutations. PARP family enzymes perform post‑translational modification functions participate death. Inhibitors PARP, ataxia telangiectasia mutant θ serve key specific subtypes. PARP1 as an important biological marker predict therapeutic evaluate prognosis patients ccRCC polybromo mutation. Therefore, progression hold promises certain types

Language: Английский

Citations

10
Identification of Poly(ADP-ribose) Polymerase 9 (PARP9) as a Potent Suppressor for Mycobacterium tuberculosis Infection DOI
Zhenyu Zhu, Shufeng Weng, Fen Zheng

et al.

Phenomics, Journal Year: 2023, Volume and Issue: 4(2), P. 158 - 170

Published: Sept. 4, 2023

Language: Английский

Citations

5
SERBP1 interacts with PARP1 and is present in PARylation-dependent protein complexes regulating splicing, cell division, and ribosome biogenesis DOI Open Access

Kira Breunig,

Xiufen Lei,

Mauro Montalbano

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 25, 2024

ABSTRACT RNA binding proteins (RBPs) containing intrinsically disordered regions (IDRs) are present in diverse molecular complexes where they function as dynamic regulators. Their characteristics promote liquid-liquid phase separation (LLPS) and the formation of membraneless organelles such stress granules nucleoli. IDR-RBPs particularly relevant nervous system their dysfunction is associated with neurodegenerative diseases brain tumor development. Serpine1 mRNA-binding protein 1 (SERBP1) a unique member this group, being mostly lacking canonical RNA-binding domains. We defined SERBP1’s interactome, uncovered novel roles splicing, cell division ribosomal biogenesis, showed its participation pathological Tau aggregates Alzheimer’s brains. SERBP1 preferentially interacts other G-quadruplex (G4) binders, implicated different stages gene expression, suggesting that G4 critical component settings. Similarly, we identified important associations between PARP1/polyADP-ribosylation (PARylation). PARP1 factors influences PARylation. Moreover, which participates contain PARylated PAR binders. Based on these results, propose feedback regulatory model PARylation, while PARylation modulates functions complexes.

Language: Английский

Citations

1
SERBP1 interacts with PARP1 and is present in PARylation-dependent protein complexes regulating splicing, cell division, and ribosome biogenesis DOI Creative Commons

Kira Breunig,

Xiufen Lei,

Mauro Montalbano

et al.

eLife, Journal Year: 2024, Volume and Issue: unknown

Published: July 2, 2024

RNA binding proteins (RBPs) containing intrinsically disordered regions (IDRs) are present in diverse molecular complexes where they function as dynamic regulators. Their characteristics promote liquid-liquid phase separation (LLPS) and the formation of membraneless organelles such stress granules nucleoli. IDR-RBPs particularly relevant nervous system their dysfunction is associated with neurodegenerative diseases brain tumor development. Serpine1 mRNA-binding protein 1 (SERBP1) a unique member this group, being mostly lacking canonical RNA-binding domains. We defined SERBP1's interactome, uncovered novel roles splicing, cell division ribosomal biogenesis, showed its participation pathological Tau aggregates Alzheimer's brains. SERBP1 preferentially interacts other G-quadruplex (G4) binders, implicated different stages gene expression, suggesting that G4 critical component settings. Similarly, we identified important associations between PARP1/polyADP-ribosylation (PARylation). PARP1 factors influences PARylation. Moreover, which participates contain PARylated PAR binders. Based on these results, propose feedback regulatory model PARylation, while PARylation modulates functions complexes.

Language: Английский

Citations

1