Ageing Research Reviews, Journal Year: 2024, Volume and Issue: unknown, P. 102631 - 102631
Published: Dec. 1, 2024
Language: Английский
Nature Reviews Bioengineering, Journal Year: 2025, Volume and Issue: unknown
Published: March 20, 2025
Language: Английский
Citations
0
Renal Failure, Journal Year: 2025, Volume and Issue: 47(1)
Published: April 22, 2025
Renal ischemia-reperfusion injury (RIRI) is a common cause of acute renal injury. Studies have shown that sodium aescinate (SA) may serve as potential therapeutic agent, although its exact mechanism remains unclear. This study first evaluated the efficacy SA using mouse model. Subsequently, was elucidated through systematic bioinformatics, and finally validated in vitro vivo experiments. The results demonstrated has protective effect on function mice with RIRI. Bioinformatic analysis indicated pyroptosis pathway significantly activated during injury, immunohistochemistry showed level upregulated Administration able to reduce expression pyroptosis-related proteins (GSDMD, NLRP3, IL-1β) In experiments further confirmed exerts an anti-pyroptotic by inhibiting AKT/NLRP3 signaling pathway. Ultimately, mitigates kidney IRI suppressing failure inhibition
Language: Английский
Citations
0
Renal Failure, Journal Year: 2025, Volume and Issue: 47(1)
Published: April 23, 2025
Limb ischemia-reperfusion (I/R) results in both localized tissue harm and injury to distant organs, particularly affecting the kidneys leading acute kidney injury. This study evaluates renoprotective effect of dapagliflozin, a drug frequently prescribed for type 2 diabetes management, relation caused by limb I/R. The extent was detected through serum marker testing rat model. Oxidative stress indicators inflammatory factors were evaluated cellular models. Histological changes examined using HE staining electron microscopy. Cell pyroptosis quantified TUNEL flow cytometry. Cellular mitochondrial function analyzed with JC-1 staining. AMPK/SIRT1/NLRP3 pathway-related proteins their mRNAs assessed via western blotting RT-qPCR techniques. We showed that dapagliflozin reduced CRE, BUN, NGAL KIM-1 levels improved renal pathology rat. Additionally, significantly raised concentrations GSH-Px SOD, concurrently MDA ROS vivo vitro. It also lowered IL-6 TNF-α cell pyroptosis. Furthermore, it observed elevated AMPK SIRT1 expressions, while decreasing NLRP3, ASC, GSDMD, IL-1β, caspase-1 expressions. Notably, these effects diminished presence siRNA. Taken together, exhibits significant protective against resulting from operates inhibition activating signaling pathway.
Language: Английский
Citations
0
Frontiers in Transplantation, Journal Year: 2025, Volume and Issue: 4
Published: April 24, 2025
This Part 1 of a bipartite review commences with succinct exposition innate alloimmunity in light the danger/injury hypothesis Immunology. The model posits that an alloimmune response, along presentation alloantigens, is driven by DAMPs released from various forms regulated cell death (RCD) induced any severe injury to donor or organ, respectively. To provide strong foundation for this review, which examines RCD and as biomarkers therapeutic targets normothermic regional perfusion (NRP) machine (NMP) improve outcomes organ transplantation, key insights are presented on nature, classification, functions DAMPs, well signaling mechanisms pathways, including ferroptosis, necroptosis, pyroptosis, NETosis. Subsequently, comprehensive discussion provided major periods injuries organs associated induction precede onset response recipients. These include conditions donation after brain (DBD) circulatory (DCD). Particular emphasis placed different origins RCD-associated DBD DCD routes they use within system reach potential allografts. ends addressing another particularly critical period organs: their postischemic reperfusion following implantation into recipient—a decisive factor determining transplantation outcome. Here, focuses ischemia-induced oxidative causes generates initiate robust response.
Language: Английский
Citations
0
Chinese Journal of Integrative Medicine, Journal Year: 2024, Volume and Issue: 30(11), P. 1027 - 1034
Published: Feb. 6, 2024
Language: Английский
Citations
2
Journal of Biochemical and Molecular Toxicology, Journal Year: 2024, Volume and Issue: 38(4)
Published: April 1, 2024
Abstract Myocardial ischemia‒reperfusion injury (MI/RI) is closely related to pyroptosis. alkB homolog 5 (ALKBH5) abnormally expressed in the MI/RI models. However, detailed molecular mechanism of ALKBH5 has not been elucidated. In this study, rats and H9C2 cells served as experimental subjects received MI/R induction H/R induction, respectively. The abundance targeted molecules was evaluated using RT‐qPCR, Western blotting, immunohistochemistry, immunofluorescence, enzyme‐linked immunosorbent assay. heart functions were echocardiography, evaluated. Cell viability pyroptosis determined cell counting Kit‐8 flow cytometry, Total m6A modification measured a commercial kit, pri‐miR‐199a‐5p detected by Me‐RNA immunoprecipitation (RIP) interactions among validated RIP luciferase experiments. highly H/R‐induced rats. silencing improved inhibited reduced methylation block miR‐199a‐5p maturation inhibit its expression. TNF receptor‐associated Factor 3 (TRAF3) downstream gene miR‐199a‐5p. Furthermore, cells, inhibitor‐mediated promotion reversed silencing, TRAF3 overexpression‐mediated offset upregulation. expression enhanced promote enhance expression, thereby suppressing alleviate through decreasing
Language: Английский
Citations
2
The Anatolian Journal of Cardiology, Journal Year: 2024, Volume and Issue: unknown, P. 318 - 328
Published: Jan. 1, 2024
The incidence and mortality of cardiovascular diseases, which coronary heart disease (CHD) is a significant burden, are on the rise. Pyroptosis as an incipient programmed cell death mediated by inflammasomes can sense cytoplasmic contamination or interference typically marked intracellular swelling, plasma membrane blistering intense inflammatory cytokine release. As research pyroptosis continues to progress, there mounting evidence that vital participant in pathophysiological basis CHD. Atherosclerosis major CHD involves endothelial cells, macrophages, vascular smooth muscle other immune often association with release pro-inflammatory factors. When cardiomyocytes damaged, it will eventually lead failure. Previous studies have covered plays critical role In this review, we describe properties pyroptosis, summarize its contribution related targets diseases involving angina pectoris, myocardial infarction, ischemia perfusion injury failure, highlight potential drugs for different diseases.
Language: Английский
Citations
2
The Chinese Journal of Physiology, Journal Year: 2023, Volume and Issue: 66(5), P. 351 - 358
Published: Sept. 1, 2023
Aging, a crucial risk factor for ischemic heart disease, has negative impacts on cardioprotective mechanisms. As such, there is still an unmet requirement to explore potential therapies improving the outcomes of myocardial ischemia/reperfusion (IR) injury in elderly subjects. Here, we aimed confirm function irisin/Dendrobium nobile Lindl (DNL) combination therapy against IR aged rats, with focus involvement pyroptosis and mitophagy. Male Wistar rats (22-24 months old, 400-450 g; n = 54) underwent or sham surgery. Before operation, were pretreated irisin (0.5 mg/kg, intraperitoneally) and/or DNL (80 orally) 1 4 weeks, respectively, at corresponding groups. Cardiac function, lactate dehydrogenase (LDH) cardiac-specific isoform troponin-I (cTn-I) levels, expression proteins involved (nod-like receptor protein-3 (NLRP3), apoptosis-associated speck-like protein, c-caspase-1, GSDMD-N) mitophagy (PINK1 Parkin), pro-inflammatory cytokines levels evaluated after 24 h reperfusion. Irisin/DNL combined significantly restored cardiac decreased LDH cTn-I levels. It also downregulated pyroptosis-related proteins, upregulated PINK1 Parkin, secretion. Pretreatment Mdivi-1, as inhibitor, abolished action dual therapy. This study revealed effects irisin/DNL IR-induced showed that mechanism might be associated suppression NLRP3-related through enhancing activity PINK1/Parkin worthy further detailed studies due its alleviate upon aging.
Language: Английский
Citations
6
International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 257, P. 128558 - 128558
Published: Dec. 2, 2023
Language: Английский
Citations
6
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: Feb. 2, 2024
Abstract Background: Stroke is the second-leading global cause of death. The immune storm triggered by ischemia-reperfusion injury after stroke a crucial damaging factor. This study analyzed expression key pyroptosis genes in and their correlation with infiltration. Methods: Middle Cerebral Artery Occlusion datasets were obtained pyroptosis-related identified. Differential functional analyses performed. Differences enrichment between high-risk low-risk groups determined. After selecting differential expression, MCAO diagnostic model was constructed validated. High for cell analysis hub genes. A regulatory network miRNA built, protein domains predicted. validated rat model. Results: Twenty-five showed including four ( WISP2 , MELK SDF2L1 AURKB ). high- significant differences . In infiltration analysis, 12 cells exhibited samples. Further demonstrated positive correlations gene cell-activated dendritic group natural killer group. Conclusion: identified genes, elevated closely associated Analysis inflammatory types provides theoretical basis predicting ischemic risk levels treatment.
Language: Английский
Citations
1