Correlation of Functional and Structural Outcomes with Serum Antibody Profiles in Patients with Neovascular Age-Related Macular Degeneration Treated with Ranibizumab and Healthy Subjects: A Prospective, Controlled Monocenter Trial DOI Open Access
Christina A. Korb,

Eva Gerstenberger,

Katrin Lorenz

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(23), P. 7033 - 7033

Published: Nov. 21, 2024

Background: Age-related macular degeneration (AMD) is a multifactorial disorder, and there growing evidence of immunological involvement in its pathogenesis. To address this, we aimed to identify biomarker candidates related retinal antigens patients with neovascular AMD treated ranibizumab healthy subjects. Materials Methods: This study was designed as prospective, open, parallel-group, interventional, single-center phase IV trial. Fifty subjects twenty volunteers were enrolled. The primary objective assess the efficacy intravitreally (IVT) administered terms change best-corrected visual acuity all subtypes subgroup pretreated A secondary same central thickness (CRT) Another antibodies against last correlate functional structural parameters identified differentiate between initial deferred responders IVT ranibizumab. Serum analyzed using customized antigen microarrays containing 58 antigens. Results: After 12 weeks treatment, gained 4.02 letters on average. measured complete population significantly (p < 0.001) decreased at Week 24 compared baseline measurement, mean CRT dropped from 393.4 296.8 µm. significant increase following autoantibodies detected control group 24, well 24: targeting proteins serotransferrin, opioid growth factor receptor, 60 kDa chaperonin 2, neurotrophin-4, dermcidin, clusterin vascular endothelial factor. Conclusions: present trial able confirm treatment AMD, treatment-naïve benefitted most. Up- downregulations observed over course Some seemed have fair correlation classification responders.

Language: Английский

Stratification of the Extent of Visual Impairment Identifies Sex-Specific Degenerative Changes in Retinal Structure and Function during Aging DOI Creative Commons
Genea Edwards, Sean M. Riordan, Cornelia Buchholz

et al.

Journal of Integrative Neuroscience, Journal Year: 2025, Volume and Issue: 24(3)

Published: March 4, 2025

Background: Initial manifestations of neurodegenerative ocular conditions, including age-related macular degeneration (AMD) and glaucoma, often remain undetected in the early stages can begin after age 50 years with likelihood gradually increasing each year thereafter. This study aimed to explore variances visual retinal function anatomy among C57BL/6J mice, aiming pinpoint differences between biological sex factors that potentially lead onset vision impairment. Methods: A longitudinal evaluated acuity (VA) contrast sensitivity (CS) using optomotor reflex (OMR), function, encompassing scotopic photopic measurements, was recorded by electroretinogram (ERG) at 12 months age. Tissue subsequently harvested for histological analysis, complementing vivo findings. Disparities were observed individual male female necessitating categorization impairment levels investigate further sex-specific study’s aging population. Comparisons degree conducted ANOVA followed Tukey’s or Bonferroni’s post-hoc corrections unpaired t-tests. Pearson correlation analysis determined association factors. Results: Sex-related disparities found (n = 13) 18) mice aged 5–12 months. Eyes categorized impairment: normal vision, low, moderate, severe loss end study. Male differed mean sensitivity, indicating less fine detail moving stimuli (11–12 old, p < 0.001). Spectral-domain optical coherence tomography (SD-OCT) revealed a thinner outer nuclear layer (p 0.0001), although this did not vary across different ERG indicated slower responses 0.05), while histology showed significant reduction inner plexiform thickness 0.0001). Conversely, exhibited greater thinning photoreceptor when unimpaired 0.01). Conclusions: The shows extent influence health, layers differentially changing over time.

Language: Английский

Citations

0
Cell-specific localization of β-synuclein in the mouse retina DOI

Wenhui Zhong,

Qingwen Yang, Fenglan Wang

et al.

Brain Structure and Function, Journal Year: 2024, Volume and Issue: 229(5), P. 1279 - 1298

Published: May 4, 2024

Language: Английский

Citations

0
Retinal ganglion cell type-specific expression of synuclein family members revealed by scRNA-sequencing DOI Creative Commons
Qingwen Yang, Lin Liu, He Fang

et al.

International Journal of Medical Sciences, Journal Year: 2024, Volume and Issue: 21(8), P. 1472 - 1490

Published: Jan. 1, 2024

Synuclein family members (Snca, Sncb, and Scng) are expressed in the retina, but their precise locations roles poorly understood. We performed an extensive analysis of single-cell transcriptome healthy injured retinas to investigate expression patterns roles. observed all synuclein retinal ganglion cells (RGCs), which remained consistent across species (human, mouse, chicken). unveiled differential Snca distinct clusters (highly most), while Sncb Sncg displayed uniform clusters. Further, we a decreased RGCs following traumatic axonal injury. However, proportion α-Syn-positive intrinsically photosensitive (ipRGCs) ipRGCs unaltered. Lastly, identified changes communication preceding cell death, with particular significance pleiotrophin-nucleolin (Ptn-Ncl) neural adhesion molecule signaling pathways, where differences were pronounced between varying levels Snca. Our study employs innovative approach using scRNA-seq characterize health cells, specifically focusing on RGC subtypes, advances our knowledge physiology pathology.

Language: Английский

Citations

0
Correlation of Functional and Structural Outcomes with Serum Antibody Profiles in Patients with Neovascular Age-Related Macular Degeneration Treated with Ranibizumab and Healthy Subjects: A Prospective, Controlled Monocenter Trial DOI Open Access
Christina A. Korb,

Eva Gerstenberger,

Katrin Lorenz

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(23), P. 7033 - 7033

Published: Nov. 21, 2024

Background: Age-related macular degeneration (AMD) is a multifactorial disorder, and there growing evidence of immunological involvement in its pathogenesis. To address this, we aimed to identify biomarker candidates related retinal antigens patients with neovascular AMD treated ranibizumab healthy subjects. Materials Methods: This study was designed as prospective, open, parallel-group, interventional, single-center phase IV trial. Fifty subjects twenty volunteers were enrolled. The primary objective assess the efficacy intravitreally (IVT) administered terms change best-corrected visual acuity all subtypes subgroup pretreated A secondary same central thickness (CRT) Another antibodies against last correlate functional structural parameters identified differentiate between initial deferred responders IVT ranibizumab. Serum analyzed using customized antigen microarrays containing 58 antigens. Results: After 12 weeks treatment, gained 4.02 letters on average. measured complete population significantly (p < 0.001) decreased at Week 24 compared baseline measurement, mean CRT dropped from 393.4 296.8 µm. significant increase following autoantibodies detected control group 24, well 24: targeting proteins serotransferrin, opioid growth factor receptor, 60 kDa chaperonin 2, neurotrophin-4, dermcidin, clusterin vascular endothelial factor. Conclusions: present trial able confirm treatment AMD, treatment-naïve benefitted most. Up- downregulations observed over course Some seemed have fair correlation classification responders.

Language: Английский

Citations

0