Biomolecules, Journal Year: 2023, Volume and Issue: 13(8), P. 1177 - 1177
Published: July 28, 2023
Teeth are unique and multifaceted tissues that necessary for routine functions, such as crushing food constructing articulated sounds, well esthetic symbols [...].
Language: Английский
Pflügers Archiv - European Journal of Physiology, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 12, 2024
Abstract In odontoblasts, intracellular Ca 2+ signaling plays key roles in reactionary dentin formation and generation of dentinal pain. Odontoblasts also express several G s protein-coupled receptors that promote production cyclic AMP (cAMP). However, the crosstalk between cAMP signaling, as well role cellular functions remains unclear. this study, we measured levels free concentration ([Ca ] i ). We investigated effect on mineralization by odontoblasts. presence extracellular , application forskolin (adenylyl cyclase activator) or isoproterenol (G beta-2 adrenergic receptor agonist) increased [Ca The increases could not be observed removing indicating is capable to activate entry. Forskolin-induced increase was inhibited a protein kinase A inhibitor sensitive Gd 3+ 2APB, Zn but verapamil, ML218, La . immunofluorescence analyses, odontoblasts were immunopositive for calcium homeostasis modulator 1 (CALHM1), which found close ionotropic ATP subtype, P2X 3 receptors. When CALHM1 knocked down, forskolin-induced suppressed. Alizarin red von Kossa staining showed decreased mineralization. These findings suggest activation adenylyl elicited level influx via Subsequent cAMP-dependent mediated addition, pathway negatively dentinogenesis.
Language: Английский
Citations
0
Biomolecules, Journal Year: 2023, Volume and Issue: 13(8), P. 1177 - 1177
Published: July 28, 2023
Teeth are unique and multifaceted tissues that necessary for routine functions, such as crushing food constructing articulated sounds, well esthetic symbols [...].
Language: Английский
Citations
0