Integrated Transcriptomic and Proteomic Analyses of Low-Nitrogen-Stress Tolerance and Function Analysis of ZmGST42 Gene in Maize

Antioxidants, Journal Year: 2023, Volume and Issue: 12(10), P. 1831 - 1831

Published: Oct. 5, 2023

Maize (Zea mays L.) is one of the major staple crops providing human food, animal feed, and raw material support for biofuel production. For its growth development, maize requires essential macronutrients. In particular, nitrogen (N) plays an important role in determining final yield quality a crop. However, excessive application N fertilizer causing serious pollution land area water bodies. Therefore, cultivating high-yield low-N-tolerant varieties crucial minimizing nitrate Here, based on analysis leaf transcriptome proteome at grain filling stage, we identified 3957 differentially expressed genes (DEGs) 329 abundant proteins (DAPs) from two hybrids contrasting stress tolerance (low-N-tolerant XY335 low-N-sensitive HN138) screened four sets low-N-responsive through Venn diagram analysis. We 761 DEGs (253 up- 508 down-regulated) specific to XY335, whereas 259 (198 61 were HN138, 59 (41 18 shared between cultivars under low-N-stress conditions. Meanwhile, among DAPs, thirty unique three DAPs low-N treatment. Key those genes/proteins leucine-rich repeat protein, DEAD-box ATP-dependent RNA helicase family proteins, copper transport photosynthesis-related proteins. These involved MAPK signaling pathway, regulating membrane lipid peroxidation, photosynthesis. Our results may suggest that better tolerates than possibly robust sensing signaling, amplified protein phosphorylation response, increased photosynthesis efficiency, as well down-regulation 'lavish' or redundant minimize demand. Additionally, glutathione transferase 42 (ZmGST42) performed physiological biochemical characterizations wild-type (B73) gst42 mutant seedling stage. Resultantly, exhibited stronger low line. findings provide understanding molecular mechanisms underlying during stage reveal key candidate low-N-tolerance breeding maize.

Language: Английский

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In‐silico identification of putatively functional intergenic small open reading frames in the cucumber genome and their predicted response to biotic and abiotic stresses

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: 47(12), P. 5330 - 5342

Published: Aug. 27, 2024

The availability of high-throughput sequencing technologies increased our understanding different genomes. However, the genomes all living organisms still have many unidentified coding sequences. number missing small open reading frames (sORFs) is due to length threshold used in most gene identification tools, which true genic and, more importantly and surprisingly, intergenic regions. Scanning cucumber genome regions revealed 420 723 sORF. We excluded 3850 sORF with similarities annotated proteins. To propose functionality remaining 416 873 sORF, we calculated their codon adaptation index (CAI). found 398 937 novel (nsORF) CAI ≥ 0.7 that were further for downstream analysis. Searching against Rfam database 109 nsORFs similar multiple RNA families. Using SignalP-5.0 NLS, identified 11 592 signal peptides. Five predicted proteins interacting Meloidogyne incognita Powdery mildew selected using published transcriptome data host-pathogen interactions. Gene ontology enrichment interpreted function those proteins, illustrating nsORFs' expression could contribute cucumber's response biotic abiotic stresses. This research highlights importance previously overlooked provides insights into potential functions.

Language: Английский

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Transcriptional responses of durum wheat to chronic chromium exposure reveal candidate proteins involved in metal detoxification and compartmentalization

Environmental and Experimental Botany, Journal Year: 2024, Volume and Issue: 228, P. 105953 - 105953

Published: Sept. 3, 2024

Chromium phytotoxicity results in relevant alterations to plant physiology, gene expression, and genomic DNA methylation at a transgenerational level. Herein, transcriptional responses of durum wheat (Triticum turgidum L.) chronic chromium exposure were explored roots leaves by RNA-seq approach. Plants grown all the time hydroponic system supplemented with 2.5 10 µM compared unstressful control plants, assessing biomass seed yield analyses after senescence. Then, transcriptomic analysis was performed these plants kept under 50 days onset exposure. The concentrations used considered lowest dose sufficient alter expression without impeding development, while sampling reflected effects pre-harvest phase long-lasting defense mechanisms. Root leaf samples from stress analyzed, generating 12 libraries. In total, 965 810 transcripts found be differentially expressed, respectively, root response stress. roots, changes noted primary secondary metabolism, redox homeostasis, protein modification, solute transport, nutrient uptake, external stimuli responses. Meanwhile, primarily hormone-related pathways, chromatin modifications, cell division, modification uptake. particular, metal uptake translocation pathways studied greater emphasis identify key proteins involved transport compartmentalization. Furthermore, several genes malate-derived organic acid biosynthesis, trace transport/detoxification/chelation, vacuolar compartmentalization liked responses, some them also associated two putative clusters. Therefore, clusters are suggested as valuable biotechnological targets for future proof-of-concept studies aimed genetic engineering improve tolerance

Language: Английский

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Integration of Transcriptomics and WGCNA to Characterize Trichoderma harzianum-Induced Systemic Resistance in Astragalus mongholicus for Defense against Fusarium solani

Genes, Journal Year: 2024, Volume and Issue: 15(9), P. 1180 - 1180

Published: Sept. 8, 2024

Beneficial fungi of the genus Trichoderma are among most widespread biocontrol agents that induce a plant’s defense response against pathogens. Fusarium solani is one main pathogens can negatively affect Astragalus mongholicus production and quality. To investigate impact harzianum on responses to solani, A. roots under T. + F. (T F) treatment (F) were sampled subjected transcriptomic analysis. A differential expression analysis revealed 6361 differentially expressed genes (DEGs) responded induction. The Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment DEGs significantly clustered into resistance-related pathways, such as plant–pathogen interaction pathway, phenylpropanoid biosynthesis flavonoid isoflavonoid mitogen-activated protein kinase (MAPK) signaling plant hormone signal transduction pathway. Pathway PR1, formononetin biosynthesis, biochanin CHIB, ROS production, HSP90 may be upregulated by play important roles in disease resistance. Our study further H2O2 content was increased Formononetin had potential suppress solani. Weighted gene coexpression network (WGCNA) module, including 58 associated with One core hub gene, RPS25, found harzianum, SA (salicylic acid) ETH (ethephon). Overall, our data indicate induced systemic resistance (ISR) acquired (SAR) mongholicus. results this lay foundation for understanding molecular mechanism which induces

Language: Английский

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Proteome and metabolome of Annona crassiflora Mart. fruit and their interaction during development

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 339, P. 113809 - 113809

Published: Nov. 28, 2024

Language: Английский

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Assembly and comparative analysis of the first complete mitochondrial genome of Salix psammophila, a good windbreak and sand fixation shrub

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 2, 2024

, commonly known as the sandlive willow, is a vital shrub species within Salicaceae family, particularly significant for its ecological role in regions susceptible to desertification and sandy soils. In this study, we assembled complete

Language: Английский

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Silicon might mitigate nickel toxicity in maize roots via chelation, detoxification, and membrane transport

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 288, P. 117334 - 117334

Published: Nov. 16, 2024

Language: Английский

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