Sugar Highs: Recent Notable Breakthroughs in Glycobiology DOI

Jimin Hu,

Duc T. Huynh, Michael Boyce

et al.

Biochemistry, Journal Year: 2024, Volume and Issue: 63(22), P. 2937 - 2947

Published: Oct. 30, 2024

Glycosylation is biochemically complex and functionally critical to a wide range of processes disease states, making it vibrant area contemporary research. Here, we highlight selection notable recent advances in the glycobiology SARS-CoV-2 infection immunity, cancer biology immunotherapy, newly discovered glycosylated RNAs. Together, these studies illustrate significance glycosylation normal great promise manipulating for therapeutic benefit disease.

Language: Английский

Multi Omics Applications in Biological Systems DOI Creative Commons
Cristian D. Gutierrez Reyes, Gerardo Alejo‐Jacuinde, Benjamin Perez Sanchez

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(6), P. 5777 - 5793

Published: June 11, 2024

Traditional methodologies often fall short in addressing the complexity of biological systems. In this regard, system biology omics have brought invaluable tools for conducting comprehensive analysis. Current sequencing capabilities revolutionized genetics and genomics studies, as well characterization transcriptional profiling dynamics several species sample types. Biological systems experience complex biochemical processes involving thousands molecules. These occur at different levels that can be studied using mass spectrometry-based (MS-based) analysis, enabling high-throughput proteomics, glycoproteomics, glycomics, metabolomics, lipidomics Here, we present most up-to-date techniques utilized completion Additionally, include some interesting examples applicability multi to a variety

Language: Английский

Citations

13
Metabolomic Changes in Rat Serum after Chronic Exposure to Glyphosate-Based Herbicide DOI Creative Commons
Oluwatosin Daramola, Cristian D. Gutierrez Reyes, Jesús Chávez‐Reyes

et al.

Metabolites, Journal Year: 2024, Volume and Issue: 14(1), P. 50 - 50

Published: Jan. 13, 2024

Glyphosate-based herbicides (GBHs) have gained extensive popularity in recent decades. For many years, glyphosate has been regarded as harmless or minimally toxic to mammals due the absence of its primary target, shikimic acid pathway humans. Nonetheless, mounting evidence suggests that may cause adverse health effects humans via other mechanisms. In this study, we described metabolomic changes serum experimental rats exposed chronic GBH using highly sensitive LC-MS/MS technique. We investigated possible relationship between exposure and neurological disorders. Our findings suggest can alter spatial learning memory expression some important metabolites are linked neurophysiological disorders young rats, with female showing higher susceptibility compared males. This indicates more likely show early symptoms disorder on male rats. observed four (paraxanthine, epinephrine, L-(+)-arginine, D-arginine) showed significant involvement suggested by ingenuity analysis. conclusion, our results indicate increase risk developing

Language: Английский

Citations

7
Comparison of N- and O-Glycosylation on Spike Glycoprotein 1 of SARS-CoV-1 and MERS-CoV DOI
Yuan Tian, John F. Cipollo

Journal of Proteome Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

SARS-CoV-1 and MERS-CoV were the infective agents of 2002 2012 coronavirus outbreaks, respectively. Here, we report a comparative liquid chromatography/mass spectrometry (LC/MS) Orbitrap N- O-glycosylation glycoproteomics study recombinant S1 spike derived from these two viruses. The former was produced in HEK293 cells latter both insect cells. Both proteins highly glycosylated, with having 13 12 N-glycosites. Nearly all occupied at 85% or more. Between 2 113 unique N-glycan compositions detected each N-glycosite across three proteins. Complex N-glycans dominated cell-derived While glycosylation differs between cells, extent glycan processing glycosites similar for forms. HEK293-derived more sialylated fucosylated compared to MERS S1, while had high-mannose glycosides, particularly N-terminus near RBD. Seven 8 O-glycosites identified Mapping predicted antigenic sites reveals colocalization consistent role immune system avoidance. Glycosylation patterns differ those other reported forms such as trimeric virus-propagated forms, which has implications virus research, including vaccine development, plays function epitope structure.

Language: Английский

Citations

0
Identification of a Novel Antiviral Lectin against SARS-CoV-2 Omicron Variant from Shiitake-Mushroom-Derived Vesicle-like Nanoparticles DOI Creative Commons
Joshua Wiggins,

Shazeed-Ul Karim,

Baolong Liu

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(10), P. 1546 - 1546

Published: Sept. 30, 2024

Lectins are a class of carbohydrate-binding proteins that may have antiviral activity by binding to the glycans on virion surface interfere with viral entry. We identified novel lectin (named Shictin) from Shiitake mushroom (

Language: Английский

Citations

3
LC-MS/MS of isomeric N-and O-glycopeptides on mesoporous graphitized carbon column DOI
Oluwatosin Daramola, Sakshi Gautam, Cristian D. Gutierrez Reyes

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1317, P. 342907 - 342907

Published: June 25, 2024

Language: Английский

Citations

2
In vitro cytotoxicity and antiviral activity of aminocaproic acid against SARS-CoV-2 DOI
Timur Saliev, Shynar Tanabayeva,

Neilya Ussebayeva

et al.

Journal Of Advanced Pharmacy Education And Research, Journal Year: 2024, Volume and Issue: 14(3), P. 1 - 8

Published: Jan. 1, 2024

Language: Английский

Citations

1
Neuroglycome alterations of hippocampus and prefrontal cortex of juvenile rats chronically exposed to glyphosate-based herbicide DOI Creative Commons
Joy Solomon, Cristian D. Gutierrez Reyes, Jesús Chávez‐Reyes

et al.

Frontiers in Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Aug. 21, 2024

Introduction Glyphosate-based herbicides (GBHs) have been shown to significant neurotoxic effects, affecting both the structure and function of brain, potentially contributing development neurodegenerative disorders. Despite known importance glycosylation in disease progression, glycome profile systems exposed GBH has not thoroughly investigated. Methods In this study, we conducted a comprehensive glycomic profiling using LC-MS/MS, on hippocampus prefrontal cortex (PFC) juvenile rats orally, aiming identify glyco-signature aberrations after herbicide exposure. Results We observed changes profile, particularly fucosylated, high mannose, sialofucosylated N-glycans, which may be triggered by Moreover, found major differences N-glycan profiles between GBH-exposed group control when analyzing each gender independently, contrast analysis that included genders. Notably, behavioral test object recognition showed decreased performance female animals compared controls ( p < 0.05), while normal behavior was recorded male > 0.05). Conclusion These findings suggest glycans play role effect caused GBH. The result suggests variation influence response exposure, with potential implications for progression specifically effects GBHs. Understanding these gender-specific responses could enhance knowledge mechanisms underlying GBH-induced toxicity its impact brain health. Overall, our study represents first detailed N-glycome PFC chronically alterations expression structures associated chronic highlighting further research area.

Language: Английский

Citations

0
Sugar Highs: Recent Notable Breakthroughs in Glycobiology DOI

Jimin Hu,

Duc T. Huynh, Michael Boyce

et al.

Biochemistry, Journal Year: 2024, Volume and Issue: 63(22), P. 2937 - 2947

Published: Oct. 30, 2024

Glycosylation is biochemically complex and functionally critical to a wide range of processes disease states, making it vibrant area contemporary research. Here, we highlight selection notable recent advances in the glycobiology SARS-CoV-2 infection immunity, cancer biology immunotherapy, newly discovered glycosylated RNAs. Together, these studies illustrate significance glycosylation normal great promise manipulating for therapeutic benefit disease.

Language: Английский

Citations

0