Antidepressant Effect of Enzymatic Porcine Placenta Hydrolysate in Repeated Immobilization Stress-Induced Ovariectomized Female Mice DOI Creative Commons
Minsook Ye,

Sharon Nguyen,

Min Kim

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(6), P. 6121 - 6138

Published: June 17, 2024

When postmenopausal women are under stress conditions, this exacerbates mood disorders and issues with neuroimmune systems. The porcine placenta is known to relieve menopausal depression in clinical trials, but its underlying mechanisms for anti-inflammatory functions remain poorly defined. present study was designed examine the effects of enzymatic hydrolysate (EPPH) on LPS-induced levels nitric oxide (NO), prostaglandin E2 (PGE2), corticosterone (CORT), pro-inflammatory cytokine interleukin-1 beta (IL-1β) RAW 264.7 macrophage cells. In addition, neurite outgrowth PC12 cells evaluated EPPH growth. To mimic symptoms menopause-related depression, a stressed ovariectomized (OVX) female mouse model used evaluate antidepressant EPPH. mice were randomly divided into five groups: (1) sham-operated (Sham) group, (2) OVX + repeated saline-treated (OVX ST) (3) estradiol (0.2 mg/kg)-treated (positive control) (4) (300 (300) (5) (1500 (1500) group. Female repeatedly immobilization-stressed 2 weeks (2 h/day). A tail suspension test conducted 13th day, followed by forced swimming 14th day assess After behavioral tests, CORT, PGE2, IL-1β evaluated. c-Fos expression paraventricular nucleus (PVN) using immunohistochemistry. concentrations NO, stimulated LPS significantly reduced via addition promoted compared that controls. test, duration immobility treated 1500 ST group had decreased c-Fos-positive neurons PVN CORT serum Sham These results suggested high dose administration induced antidepressant-like effect downregulating IL-1β, PGE2 through reducing regions.

Language: Английский

Preconditioning of Mesenchymal Stem Cells Enhances the Neuroprotective Effects of Their Conditioned Medium in an Alzheimer’s Disease In Vitro Model DOI Creative Commons
Tatiana Tolstova, Ekaterina Dotsenko,

Natalia G. Luzgina

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(10), P. 2243 - 2243

Published: Oct. 2, 2024

Alzheimer's disease (AD) develops as a result of oxidative damage to neurons and chronic inflammation microglia. These processes can be influenced by the use conditioned medium (CM) derived from mesenchymal stem cells (MSCs). The CM contains wide range factors that have neurotrophic, antioxidant, anti-inflammatory effects. In addition, therapeutic potential further enhanced pretreating MSCs increase their paracrine activity. current study aimed investigate neuroprotective effects MSCs, which were either activated TLR3 ligand or exposed CoCl

Language: Английский

Citations

1
The Role and Mechanism of Ambra1-Mediated Mitophagy in TDCPP-Exposed Mouse Hippocampal Neurons DOI
Xiaowei Zhang,

Chuzhi Lin,

Hengfang Hu

et al.

Neurochemical Research, Journal Year: 2024, Volume and Issue: 49(9), P. 2453 - 2468

Published: June 8, 2024

Language: Английский

Citations

0
Antidepressant Effect of Enzymatic Porcine Placenta Hydrolysate in Repeated Immobilization Stress-Induced Ovariectomized Female Mice DOI Creative Commons
Minsook Ye,

Sharon Nguyen,

Min Kim

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(6), P. 6121 - 6138

Published: June 17, 2024

When postmenopausal women are under stress conditions, this exacerbates mood disorders and issues with neuroimmune systems. The porcine placenta is known to relieve menopausal depression in clinical trials, but its underlying mechanisms for anti-inflammatory functions remain poorly defined. present study was designed examine the effects of enzymatic hydrolysate (EPPH) on LPS-induced levels nitric oxide (NO), prostaglandin E2 (PGE2), corticosterone (CORT), pro-inflammatory cytokine interleukin-1 beta (IL-1β) RAW 264.7 macrophage cells. In addition, neurite outgrowth PC12 cells evaluated EPPH growth. To mimic symptoms menopause-related depression, a stressed ovariectomized (OVX) female mouse model used evaluate antidepressant EPPH. mice were randomly divided into five groups: (1) sham-operated (Sham) group, (2) OVX + repeated saline-treated (OVX ST) (3) estradiol (0.2 mg/kg)-treated (positive control) (4) (300 (300) (5) (1500 (1500) group. Female repeatedly immobilization-stressed 2 weeks (2 h/day). A tail suspension test conducted 13th day, followed by forced swimming 14th day assess After behavioral tests, CORT, PGE2, IL-1β evaluated. c-Fos expression paraventricular nucleus (PVN) using immunohistochemistry. concentrations NO, stimulated LPS significantly reduced via addition promoted compared that controls. test, duration immobility treated 1500 ST group had decreased c-Fos-positive neurons PVN CORT serum Sham These results suggested high dose administration induced antidepressant-like effect downregulating IL-1β, PGE2 through reducing regions.

Language: Английский

Citations

0