A critical role of a plant-specific TFIIB-related protein, BRP1, in salicylic acid-mediated immune response DOI Creative Commons
Binjie Xu, Baofang Fan, Zhixiang Chen

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: July 30, 2024

An integral part of plant immunity is transcription reprogramming by concerted action specific factors that activate or repress genes through recruitment release RNA polymerase II (Pol II). Pol assembled into holoenzyme at the promoters association with a group general including factor IIB (TFIIB) to transcription. Unlike other eukaryotic organisms, plants have large family TFIIB-related proteins 15 members in Arabidopsis several plant-specific (BRPs). Molecular genetic analysis has revealed important roles some BRPs reproductive processes. In this study, we report knockout mutants for BRP1, founding member BRP protein family, were normal growth and development, but hypersusceptible bacterial pathogen

Language: Английский

Regulation of Gene Expression DOI
Carolyn M. Klinge, Barbara J. Clark

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

4
Systematic characterization of the bZIP transcription factor family of Japanese plum (Prunus salicina Lindl.) and their potential role in phenolic compound biosynthesis DOI Creative Commons
J. Ibañez,

Benjamín Battistoni,

Arnau Fiol

et al.

Scientia Horticulturae, Journal Year: 2025, Volume and Issue: 341, P. 113962 - 113962

Published: Feb. 1, 2025

Language: Английский

Citations

0
Harnessing promoter elements to enhance gene editing in plants: perspectives and advances DOI Creative Commons
Nikita Gondalia, Luis Felipe Quiroz, Linyi Lai

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Summary Genome‐edited plants, endowed with climate‐smart traits, have been promoted as tools for strengthening resilience against climate change. Successful plant gene editing (GE) requires precise regulation of the GE machinery, a process controlled by promoters, which drives its transcription through interactions factors (TFs) and RNA polymerase. While constitutive promoters are extensively used in constructs, their limitations highlight need alternative approaches. This review emphasizes promise tissue/organ specific well inducible enable targeted spatiotemporal manner no effects on other tissues. Advances synthetic biology paved way creation offering refined control over expression augmenting potential GE. The integration these novel systems presents significant opportunities conditional genome editing. Moreover, advent bioinformatic artificial intelligence is revolutionizing characterization regulatory elements, enhancing our understanding roles plants. Thus, this provides insights into strategic use promoter to enhance precision, efficiency specificity GE, setting stage innovative crop improvement strategies.

Language: Английский

Citations

0
Post‐Transcriptional Regulation of Gene Expression and the Intricate Life of Eukaryotic mRNAs DOI Creative Commons
Carly L. Lancaster, Kenneth H. Moberg, Anita H. Corbett

et al.

Wiley Interdisciplinary Reviews - RNA, Journal Year: 2025, Volume and Issue: 16(2)

Published: March 1, 2025

In recent years, there has been a growing appreciation for how regulatory events that occur either co- or post-transcriptionally contribute to the control of gene expression. Messenger RNAs (mRNAs) are extensively regulated throughout their metabolism in precise spatiotemporal manner requires sophisticated molecular mechanisms cell-type-specific expression, which dictates cell function. Moreover, dysfunction at any these steps can result variety human diseases, including cancers, muscular atrophies, and neurological diseases. This review summarizes central dogma biology, focusing on post-transcriptional regulation

Language: Английский

Citations

0
Drosophila architectural proteins M1BP and Opbp cooperatively form the active promoter of a ribosomal protein gene DOI Creative Commons

I. S. Osadchiy,

Anastasia Umnova,

Galina V. Pokholkova

et al.

Epigenetics & Chromatin, Journal Year: 2025, Volume and Issue: 18(1)

Published: April 16, 2025

In Drosophila, architectural proteins are frequently found in promoters, including those of genes with extremely high expression levels, such as ribosomal protein (RPGs). The involvement several these gene regulation Drosophila has been shown, but the exact mechanisms their possible cooperative action have not fully elucidated. this study we dissected contribution Opbp and M1BP, which co-localized at RPG promoters near transcription start site, to promoter functioning. We that two domains directly interact CP190, Putzig (Pzg), Chromator (Chro) proteins, cofactors required for activation housekeeping (hk) promoters. These redundant functions vivo can tether forming open chromatin regions when artificially recruited "closed" chromatin. Additionally, observed interactions between M1BP same cofactors. transgene assay, driven by 192-bp part Rpl27A is dependent on presence least one or binding site it sufficient very activity integrated into hk cluster moderate outside cluster, while both sites even more facilitates transcription. This demonstrates different work independently cooperation fulfill partially

Language: Английский

Citations

0
Single-molecule microscopy reveals that TFIIE subunits dynamically interact with preinitiation complexes in a manner controlled by TFIIH DOI
Stephen R. Archuleta, C. Ryan Miller,

Julia A. Mirita

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 20, 2025

Abstract Transcription by RNA polymerase II (Pol II) requires general transcription factors that bind with Pol at the promoters of protein-coding genes to form preinitiation complexes (PICs). Among these is TFIIE, which recruits TFIIH PIC and stimulates kinase translocase activities TFIIH, thereby regulating fate formed PICs. In this study, we used a purified reconstituted human system single molecule total internal reflection fluorescence (smTIRF) microscopy monitor TFIIE binding dynamics in PICs under different conditions real time. We observed highly dynamic interactions two subunits (TFIIEα TFIIEβ) Measurement rate constants for on/off each subunit suggest they behave asynchronously. exclusion increased rates association dissociation both subunits, strongest effect on TFIIEα. Despite stabilization remain Additionally, disease-related TFIIEβ point mutations destabilized altered its kinetic behaviors within Our results contribute an emerging model are not static assemblies highlight important connections between structural arrangement GTFs

Language: Английский

Citations

0
Assembly of the Xrn2/Rat1–Rai1–Rtt103 termination complexes in mesophilic and thermophilic organisms DOI Creative Commons

Alzbeta Dikunova,

N. V. Noskova,

Jan H. Overbeck

et al.

Structure, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

1
A critical role of a plant-specific TFIIB-related protein, BRP1, in salicylic acid-mediated immune response DOI Creative Commons
Binjie Xu, Baofang Fan, Zhixiang Chen

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: July 30, 2024

An integral part of plant immunity is transcription reprogramming by concerted action specific factors that activate or repress genes through recruitment release RNA polymerase II (Pol II). Pol assembled into holoenzyme at the promoters association with a group general including factor IIB (TFIIB) to transcription. Unlike other eukaryotic organisms, plants have large family TFIIB-related proteins 15 members in Arabidopsis several plant-specific (BRPs). Molecular genetic analysis has revealed important roles some BRPs reproductive processes. In this study, we report knockout mutants for BRP1, founding member BRP protein family, were normal growth and development, but hypersusceptible bacterial pathogen

Language: Английский

Citations

0