Squaraine-Peptide Conjugates as Efficient Reporters of Neutrophil Extracellular Traps-Mediated Chronic Inflammation

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

The excessive and uncontrolled release of neutrophil extracellular traps (NETs) is increasingly linked to the pathogenesis various inflammatory diseases, cardiovascular disorders, cancers. Real-time, non-invasive detection NETs crucial for understanding their role in disease progression developing targeted therapies. Current methods often lack necessary specificity resolution, particularly vivo ex settings. To address this, we have developed novel near-infrared squaraine-peptide conjugates by rational molecular design as reporters NETosis targeting protease activity elastase (NE). These self-quenching, cell-impermeable probes enable precise real-time imaging NETs. Förster resonance energy transfer (FRET)-based probe, Hetero-APA, demonstrated high detecting vitro vivo, generating strong fluorescence NETs-rich environments. overcome limitations FRET-based imaging, designed SQ-215-NETP, a non-FRET-based probe that covalently binds NE. SQ-215-NETP achieved an unprecedented resolution 90 nm/pixel human coronary thrombi, marking first report such with low weight probe. Additionally, effectively detected flow cytometry. results highlight potential these detection, offering promising tools enhanced diagnostics therapeutic strategies managing NET-mediated diseases

Language: Английский

---

Development of an enzyme-linked immunosorbent assay (ELISA) for determining neutrophil elastase (NE) – a potential useful marker of multi-organ damage observed in COVID-19 and post-Covid-19 (PCS)

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 25, 2025

The ongoing post-COVID-19 syndrome (PCS) epidemic, causing complications of diverse etiology, necessitates the search for new diagnostic markers and development widely accessible methods their detection. This would enable prognosis PCS progression faster implementation targeted treatments. One potential marker is neutrophil elastase (NE), whose elevated levels in blood during may result from organ damage caused by increased secretion severe inflammatory mediators or amyloidosis resulting interaction NE with SARS-CoV-2. aim this publication to present a step-by-step method designing an enzymatic ELISA test, enabling quantitative assessment serum patients. was measured using designed test. study outlines all steps necessary optimizing including selection standards, primary secondary antibodies, dilutions. Using were demonstrated patients advanced-stage diabetic nephropathy after symptomatic COVID-19, compared relative group sampled before COVID-19. undertaken efforts enabled test high performance parameters (initially set sensitivity: ≥40 pg/μL; intra-assay precision: 7%; inter-assay precision <20%). No significant cross-reactivity other tested proteins observed. Serial dilution plasma samples resulted proportional decrease signal intensity.

Language: Английский

---

Challenges and Opportunities for Post-COVID Pulmonary Disease: A Focused Review of Immunomodulation

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3850 - 3850

Published: April 18, 2025

The resolution of the recent COVID-19 pandemic still requires attention, since consequences having suffered infection, even in mild cases, are associated with several acute and chronic pathological conditions referred to as post-COVID syndrome (PCS). PCS often manifests pulmonary disease and, up 9% a more serious complication known post-COVID-19 fibrosis (PC19-PF), which has similar clinical course idiopathic (IPF). Generating knowledge provide robust evidence about benefits different therapeutic strategies treat effects can new insights amplify options for these patients. We present found after scoping review, following extended PRIMSA guidelines, use immunomodulators PCS. start brief description immunomodulatory properties relevant drugs, their clinically proven efficacy viral infections inflammatory conditions, during pandemic. emphasize need well-designed trials improve our understanding physiopathology PC19-PF also determine safety candidate treatments.

Language: Английский

---

Nets in fibrosis: Bridging innate immunity and tissue remodeling

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 137, P. 112516 - 112516

Published: June 20, 2024

Language: Английский

---

Anetoderma in the Setting of a Hematologic Abnormality and COVID-19 Infection

American Journal of Dermatopathology, Journal Year: 2024, Volume and Issue: 46(11), P. e104 - e105

Published: Aug. 14, 2024

Abstract: Anetoderma is an uncommonly reported dermatosis clinically characterized by discrete skin lesions resulting from elastic fiber loss within the dermis. Although primary anetoderma has been considered idiopathic, a range of associated disorders, particularly prothrombotic abnormalities, have reported. We present 79-year-old woman with heterozygous Factor V Leiden mutation who developed shortly after COVID-19 infection complicated thromboembolic event. Our patient adds to literature on occurrence in setting and/or disorder.

Language: Английский

---