Preliminary Insights into the Antigenotoxic Potential of Lemon Essential Oil and Olive Oil in Human Peripheral Blood Mononuclear Cells

Plants, Journal Year: 2024, Volume and Issue: 13(12), P. 1623 - 1623

Published: June 12, 2024

Lemon essential oil, derived from Citrus limon, possesses diverse health-promoting properties, including antioxidant, antimicrobial, and mood-enhancing effects. Despite its traditional use in aromatherapy complementary medicine, there is a need for comprehensive investigations into therapeutic potential, particularly mitigating DNA damage supporting health palliative care settings. This study aimed to evaluate the antigenotoxic effects of lemon oil human peripheral blood mononuclear cells explore potential applications care. Treatment with significantly reduced damage, 1% w/v 3.13% tail demonstrating greater efficacy. Furthermore, attenuated streptonigrin-induced suggesting protective effect against oxidative stress, especially at 3% w/v, 11.81% tail. Compared olive treatment, was lower streptonigrin treatment alone, which had 47.06% tail, while resulted 36.88% These results can be attributed main constituents: limonene oleic acid oil. suggest role stress genomic stability. Further research warranted elucidate mechanisms action clinical

Language: Английский

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Positive Correlation Between Serum Limonene Levels and Muscle Health in a Representative Adult Population in the United States

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(1), P. 74 - 74

Published: Jan. 10, 2025

Background/Objectives: Monoterpenes, a class of organic compounds with the molecular formula C10H16, have garnered significant attention for their potential medicinal benefits. Emerging evidence suggests they may positively influence skeletal muscle function. However, impact monoterpene exposure on strength and mass in humans remains unclear. Methods: To explore this relationship, we analyzed data from 1202 adults (aged ≥ 18 years) who participated 2013–2014 National Health Nutrition Examination Survey (NHANES), focusing serum levels three specific monoterpenes—α-pinene, β-pinene, limonene—and association hand grip lean mass. Results: Our analysis revealed that, except test 2 1, all measures showed positive correlation ln-limonene levels. The β coefficient combined was 2.409 (S.E. = 0.891, p 0.015). Positive associations were also found between limonene Appendicular Skeletal Muscle Mass Index (ASMI) 0.138 0.041, 0.004). Furthermore, ASMI significantly increased across quintiles (p trend 0.005 0.006, respectively). none clinically defined low or strength. Conclusions: findings suggest plausible to limonene, strength, among United States. Further investigation is needed fully understand underlying mechanisms medical significance association.

Language: Английский

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Chemical and Biological Study of the Essential Oil Isolated from Fruits of Citrus x limonia

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 705 - 705

Published: Feb. 25, 2025

Citrus x limonia is an aromatic species, known locally in Ecuador as limón mandarina or chino. In the present study, chemical composition and biological activity of essential oil isolated from this species were determined. The was extracted through hydrodistillation. enantiomeric distribution determined by gas chromatography. Antimicrobial using broth microdilution method against tree Gram-positive cocci, a bacillus, four Gram-negative bacilli, fungus, yeast. antioxidant ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) DPPH (2,2-diphenyl-1-picrylhydrazyl) methods. spectrophotometric used to determine anticholinesterase activity. oil, thirty-nine compounds identified, which represented 99.35% total composition. Monoterpene hydrocarbons most representative group terms number (thirteen) relative abundance (91.39%). main constituents found be limonene (57.38 ± 1.09%), γ-terpinene (13.01 0.37%), β-pinene (12.04 0.63%). Five pairs enantiomers identified fruits limonia. presented minimum inhibitory concentration 4000 μg/mL Aspergillus niger. weak per method, with SC50 1.26 mg/mL. Additionally, exhibited moderate activity, IC50 203.9 1.03 µg/mL. This study provides first comprehensive analysis activities

Language: Английский

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Indirubin attenuates sepsis by targeting the EGFR/SRC/PI3K and NF-κB/MAPK signaling pathways in macrophages

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 12, 2025

Background Isatidis Folium , a botanical drug widely used in traditional medicine, is known for its anti-inflammatory properties, including heat-clearing, detoxifying, and blood-cooling effects. Although potential sepsis treatment has been suggested, the bioactive metabolites underlying mechanisms remain poorly understood. Methods Network pharmacology molecular docking were employed to identify therapeutic effects of Indirubin, major metabolite treatment. In vivo cecal ligation puncture (CLP)-induced mouse model was evaluate protective Indirubin through histopathological analysis, ELISA, biochemical assays. vitro RAW264.7 cells stimulated with LPS treated varying concentrations Indirubin. The assessed using apoptosis assays, Western blotting. Results analysis identified as EGFR SRC key targets. Experimental validation demonstrated that significantly improved survival rates, alleviated tissue injury, suppressed inflammatory responses models. Mechanistically, inhibited LPS-induced activation EGFR/SRC/PI3K NF-κB/MAPK pathways macrophages, reducing cell death inflammation cells. Conclusion primary exerts against by targeting signaling macrophages. These findings provide mechanistic basis development multi-target agent

Language: Английский

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Study on the Protective Effect of Lianmei Decoction on LPS-Induced Sepsis Mice

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(03), P. 1035 - 1043

Published: Jan. 1, 2025

Language: Английский

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Potential roles of inhalation aromatherapy on stress-induced depression by inhibiting inflammation in the peripheral olfactory system

Neurochemistry International, Journal Year: 2025, Volume and Issue: unknown, P. 105967 - 105967

Published: March 1, 2025

Language: Английский

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Phycochemistry and pharmacological significance of filamentous cyanobacterium Spirulina sp.

Bioresources and Bioprocessing, Journal Year: 2025, Volume and Issue: 12(1)

Published: April 3, 2025

Abstract The cyanobacterium , Spirulina sp. is a photosynthetic blue-green alga with essential nutrients, vitamins nucleic acids, proteins, carbohydrates, fatty acids and pigments carotenes; phycocyanins are the significant components having immunomodulatory, anti-inflammatory properties, which used in food cosmetics industries. can play an important role human animal nutrition for potential health benefits due to their phycochemical pharmaceutical significance. This study highlights antibacterial, antifungal, antiviral, antioxidant, nephroprotective, cardioprotective, anticancer, neuroprotective, anti-aging, anti-inflammatory, immunomodulatory properties. It anti-anemic, antidiabetic, probiotic, anti-malarial, anti-obesity weight loss, anti-genotoxicity, anti-thrombic, radioprotective, detoxifying effects of Pharmaceutical studies indicate it may improve heart add treatment diabetes, obesity loss. major protecting environment by recycling wastewater providing humans animals. supply ingredients aquaculture agricultural feeds, pigments, antioxidants, omega-3 oils, among other wellness products. amino acid greatest qualititavely any plant, even higher than that soybean. Furthermore, could be future antimicrobial drug agent. Graphical

Language: Английский

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Mentha piperita Supplementation Promotes Growth, Immunity, and Disease Resistance in Nile tilapia Against Aeromonas hydrophila

Pathogens, Journal Year: 2025, Volume and Issue: 14(4), P. 378 - 378

Published: April 12, 2025

This study investigated the effects of dietary supplementation with Mentha piperita (MP) on growth, immune enhancement, and disease resistance in Nile tilapia (Oreochromis niloticus) over a 90-day period, particularly against Aeromonas hydrophila. MP was incorporated into diets at concentrations 0.0%, 0.2%, 0.4%, 0.6%. Analysis essential oil composition identified menthol derivatives as primary components, along other bioactive compounds. The results revealed that significantly enhanced growth performance, fish receiving 0.6% diet achieving highest weight gain, rate, feed efficiency. Additionally, fish’s to A. hydrophila infection, survival rate observed group. Further analyses positively influenced blood parameters, improving RBC WBC counts, hemoglobin levels, well serum immunoglobulin M phagocytic activity. also mitigated oxidative stress by increasing antioxidant enzyme activity reducing malondialdehyde levels. Moreover, concentration maintained intestinal integrity bacterial damage. Gene expression analysis showed upregulated insulin-like factor 1, suggesting potential mechanism for improved growth. Interestingly, downregulated inflammatory gene nuclear kappa B before challenge, while its remained more post-challenge compared control. These findings highlight an effective additive enhances rates tilapia, boosts immunity diseases, improves their overall health.

Language: Английский

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Volatiles in Feces of Rats Infected with the Tapeworm Hymenolepis diminuta

Journal of Parasitology, Journal Year: 2025, Volume and Issue: 111(2)

Published: April 21, 2025

It has been known for over 25 yr that feces from rats infected with the tapeworm Hymenolepis diminuta are more attractive to tenebrionid beetle intermediate hosts than uninfected rats. Although it demonstrated a volatile chemical (or chemicals) in rat attracts host Tribolium confusum, chemical(s) have never identified. This study aimed identify and compare volatiles of before after infection H. diminuta. Four 8-wk-old female Sprague Dawley were Fecal samples pre- postinfection collected, trapped by vacuum on polymer adsorbent identified gas chromatography-mass spectrometry analysis. Limonene was detected preinfection but not feces. Five carboxylic acids isolated only Butanoic acid found greatest relative amount, also 3-methylbutanoic acid, 2-methylbutanoic pentanoic hexanoic found. is first report identifying

Language: Английский

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Chemical Profiling and Assessment of Analgesic and Anti-Inflammatory Activity of Ammoides verticillata Essential Oil: In Vitro, In Vivo, and In Silico Studies

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(5), P. 635 - 635

Published: April 27, 2025

Background/Objectives: Essential oils are increasingly recognized for their therapeutic potential, yet Ammoides verticillata essential oil (AVEO) remains relatively unexplored, particularly its anti-inflammatory and analgesic properties. This study aimed to profile AVEO’s chemical composition evaluate antioxidant, anti-inflammatory, effects, with a focus on novel pharmacological actions. Methods: The of AVEO was determined using GC-MS analysis, antioxidant capacity assessed through in vitro assays. Furthermore, the potential investigated carrageenan-induced paw edema model rats, complemented by inhibition assays cyclooxygenase (COX) enzymes. effects were evaluated acetic acid-induced writhing tail immersion tests. Additionally, computational performed explore binding affinity major constituents COX-2. Results: analysis revealed rich monoterpene dominated carvacrol (32.51%). It found that exhibited significant activity. Similarly, vivo, showed achieving percentage 52.23% at 200 mg/kg, comparable diclofenac, along potent COX-2 observed (IC50 = 1.51 ± 0.20, SI 5.56). Moreover, tests demonstrated dose-dependent pain relief, which dose mg/kg significantly prolonged latency 14.00 1.45 s markedly reduced abdominal constriction 21.17 1.62. Computational further corroborated high thymol (−7.381 −6.939 Kcal/mol, respectively). Conclusions: These findings underscore as promising agent managing inflammation pain.

Language: Английский

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