Lithium Induces Oxidative Stress, Apoptotic Cell Death, and G2/M Phase Cell Cycle Arrest in A549 Lung Cancer Cells DOI Creative Commons
Pearl Ramushu,

Dikgale D. Mangoakoane,

Raymond Tshepiso Makola

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(8), P. 1797 - 1797

Published: April 17, 2025

Lithium has been identified more than six decades ago as a preferred treatment option for manic depression. Due to its affordability, stability, minimal side effects, and immunomodulatory recent studies on lithium have focused potential anticancer properties possible mechanisms of action. Lung cancer ranks the highest main cause death in males high mortality rates with low survival rates. In this study, lung adenocarcinoma (A549) cells were treated various concentrations chloride evaluate inflammatory properties. The vitro cytotoxic effects assessed using MTT [3-(4, 5-dimethythiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay, Muse® cell death, cycle analysis. nitric oxide oxidative stress flow cytometry assays used monitor inflammation profiles lithium-treated cells. viability assay showed safe use LiCl noncancerous RAW 264.7 macrophage below concentration 40 mM. reduced viability, induced late apoptotic disrupted normal progression dose-dependent manner, leading arrest S G2/M phases A549 induction by is accompanied increased ROS production. This study shows that possesses some can be further investigated treatment.

Language: Английский

Lithium Induces Oxidative Stress, Apoptotic Cell Death, and G2/M Phase Cell Cycle Arrest in A549 Lung Cancer Cells DOI Creative Commons
Pearl Ramushu,

Dikgale D. Mangoakoane,

Raymond Tshepiso Makola

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(8), P. 1797 - 1797

Published: April 17, 2025

Lithium has been identified more than six decades ago as a preferred treatment option for manic depression. Due to its affordability, stability, minimal side effects, and immunomodulatory recent studies on lithium have focused potential anticancer properties possible mechanisms of action. Lung cancer ranks the highest main cause death in males high mortality rates with low survival rates. In this study, lung adenocarcinoma (A549) cells were treated various concentrations chloride evaluate inflammatory properties. The vitro cytotoxic effects assessed using MTT [3-(4, 5-dimethythiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay, Muse® cell death, cycle analysis. nitric oxide oxidative stress flow cytometry assays used monitor inflammation profiles lithium-treated cells. viability assay showed safe use LiCl noncancerous RAW 264.7 macrophage below concentration 40 mM. reduced viability, induced late apoptotic disrupted normal progression dose-dependent manner, leading arrest S G2/M phases A549 induction by is accompanied increased ROS production. This study shows that possesses some can be further investigated treatment.

Language: Английский

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