Serine Hydroxymethyltransferase Modulates Midgut Physiology in Aedes aegypti Through miRNA Regulation: Insights from Small RNA Sequencing and Gene Expression Analysis DOI Creative Commons
Qian Pu,

Yujiao Han,

Zhen Su

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(5), P. 644 - 644

Published: April 30, 2025

Aedes aegypti mosquitoes are critical vectors of arboviruses, responsible for transmitting pathogens that pose significant public health challenges. Serine hydroxymethyltransferase (SHMT), a key enzyme in one-carbon metabolism, plays vital role various biological processes, including DNA synthesis, energy and cell proliferation. Although SHMT is expressed at low levels the midgut aegypti, its silencing has been shown to inhibit blood meal digestion. The precise mechanisms by which regulates physiology remain poorly understood. In this study, we employed small RNA sequencing quantitative PCR identify differentially miRNAs (DEMs) following downregulation. We focused on subset DEMs—miR-2940-5p, miR-2940-3p, miR-2941, miR-306-5p—to explore their potential functions. To further elucidate molecular underlying miRNA response downregulation, analyzed expression genes involved biogenesis pathway. Our results demonstrated several enzymes, Drosha, Dicer1, AGO1, exhibited changes upon silencing. This study provides new insights into through influences functions nutritional metabolism mosquito midgut. By linking activity regulation, our findings highlight pathway modulates physiology, offering foundation future research biology vector control strategies.

Language: Английский

Comparative analysis of the of red seaweed polysaccharides on hypertension–associated biomarkers in cadmium chloride-induced Wistar rats DOI
Chhanda Bose,

Penchala Udaya Bhanu,

Kajal Chakraborty

et al.

Algal Research, Journal Year: 2025, Volume and Issue: unknown, P. 104021 - 104021

Published: March 1, 2025

Language: Английский

Citations

0
PDGF-BB overexpressing dental pulp stem cells improve angiogenesis in dental pulp regeneration DOI Creative Commons
Wentao Jiang,

Shuhan Duan,

Weiping Li

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 24, 2025

Introduction Angiogenesis represents a critical challenge in dental pulp regeneration due to the tissue’s restricted nutrient supply through 0.5-mm apical foramen. While stem cells (DPSCs) hold regenerative potential, their limited vascularization capacity impedes clinical applications. Through Single-cell RNA sequencing (scRNA-seq) analysis of human pulp, we discovered PDGF (+) mesenchymal subset exhibiting enhanced angiogenic signatures, suggesting targeted cell selection could overcome this bottleneck. Methods ScRNA-seq identified subpopulation samples, validated multiplex immunohistochemical localization PDGF/CD73/CD31. PDGF-BB-overexpressing DPSCs were engineered via lentiviral vectors. Functional assessments included: 1) CCK-8/Edu/cell cycle/transwell assays for proliferation and migration ability 2) HUVECs co-culture models analyzing chemotaxis tube formation 3) Vascularized tissue rat kidney capsule transplants. Results Discussion The CD73 demonstrated spatial correlation with CD31 vasculature. PDGF-BB overexpression DPSCs' proliferative capacity. Co-cultured exhibited increased group. In vivo transplants generated more vascular structures containing endothelia. These findings establish engineering as an effective strategy amplify while emphasizing therapeutic value functionally-defined subpopulations regeneration.

Language: Английский

Citations

0
Serine Hydroxymethyltransferase Modulates Midgut Physiology in Aedes aegypti Through miRNA Regulation: Insights from Small RNA Sequencing and Gene Expression Analysis DOI Creative Commons
Qian Pu,

Yujiao Han,

Zhen Su

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(5), P. 644 - 644

Published: April 30, 2025

Aedes aegypti mosquitoes are critical vectors of arboviruses, responsible for transmitting pathogens that pose significant public health challenges. Serine hydroxymethyltransferase (SHMT), a key enzyme in one-carbon metabolism, plays vital role various biological processes, including DNA synthesis, energy and cell proliferation. Although SHMT is expressed at low levels the midgut aegypti, its silencing has been shown to inhibit blood meal digestion. The precise mechanisms by which regulates physiology remain poorly understood. In this study, we employed small RNA sequencing quantitative PCR identify differentially miRNAs (DEMs) following downregulation. We focused on subset DEMs—miR-2940-5p, miR-2940-3p, miR-2941, miR-306-5p—to explore their potential functions. To further elucidate molecular underlying miRNA response downregulation, analyzed expression genes involved biogenesis pathway. Our results demonstrated several enzymes, Drosha, Dicer1, AGO1, exhibited changes upon silencing. This study provides new insights into through influences functions nutritional metabolism mosquito midgut. By linking activity regulation, our findings highlight pathway modulates physiology, offering foundation future research biology vector control strategies.

Language: Английский

Citations

0