Host-Mediated Antimicrobial Effects and NLRP3 Inflammasome Modulation by Caulerpin and Its Derivatives in Macrophage Models of Mycobacterial Infections DOI Creative Commons

Maria Gabriella S. Sidrônio,

Maria Eugênia G. de Freitas,

Daniel Magalhães

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 561 - 561

Published: March 1, 2025

Caulerpin, a bis-indole alkaloid isolated from Caulerpa racemosa, has several documented pharmacological activities, including antineoplastic and antiviral properties. This study aimed to evaluate the anti-inflammatory anti-tubercular potentials of caulerpin its analogues in RAW 264.7 macrophages infected with Mycobacterium spp. Additionally, we evaluated cytokine production NLRP3 expression this infection model. Toxicity tests were performed using Vero E6 HepG2 cell lines Artemia salina. Pre-incubation cells decreased internalized M. smegmatis tuberculosis H37Ra. Furthermore, treatment smegmatis-infected reduced bacterial loads. Caulerpin CFU count bacilli H37Ra In addition, diethyl derivative notably found modulate IL-1β TNF-α model after quantifying pro-inflammatory cytokines NLRP3. derivates did not affect viability or nauplii survival toxicity studies. These findings demonstrate that exhibit activity against show promising potential for further efficacy safety evaluation.

Language: Английский

Host-Mediated Antimicrobial Effects and NLRP3 Inflammasome Modulation by Caulerpin and Its Derivatives in Macrophage Models of Mycobacterial Infections DOI Creative Commons

Maria Gabriella S. Sidrônio,

Maria Eugênia G. de Freitas,

Daniel Magalhães

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 561 - 561

Published: March 1, 2025

Caulerpin, a bis-indole alkaloid isolated from Caulerpa racemosa, has several documented pharmacological activities, including antineoplastic and antiviral properties. This study aimed to evaluate the anti-inflammatory anti-tubercular potentials of caulerpin its analogues in RAW 264.7 macrophages infected with Mycobacterium spp. Additionally, we evaluated cytokine production NLRP3 expression this infection model. Toxicity tests were performed using Vero E6 HepG2 cell lines Artemia salina. Pre-incubation cells decreased internalized M. smegmatis tuberculosis H37Ra. Furthermore, treatment smegmatis-infected reduced bacterial loads. Caulerpin CFU count bacilli H37Ra In addition, diethyl derivative notably found modulate IL-1β TNF-α model after quantifying pro-inflammatory cytokines NLRP3. derivates did not affect viability or nauplii survival toxicity studies. These findings demonstrate that exhibit activity against show promising potential for further efficacy safety evaluation.

Language: Английский

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