Mesenchymal Stem Cell Secretome: Potential Applications in Human Infertility Caused by Hormonal Imbalance, External Damage, or Immune Factors

Biomedicines, Journal Year: 2025, Volume and Issue: 13(3), P. 586 - 586

Published: Feb. 27, 2025

Mesenchymal stem cells (MSCs) are a source of wide range soluble factors, including different proteins, growth cytokines, chemokines, and DNA RNA molecules, in addition to numerous secondary metabolites byproducts their metabolism. MSC secretome can be formally divided into secretory vesicular parts, both which very important for intercellular communication involved processes such as angiogenesis, proliferation, immunomodulation. Exosomes thought have the same content function MSCs from they derived, but also number advantages over cells, low immunogenicity, unaltered functional activity during freezing thawing, lack tumor formation. In addition, pre-treatment with various inflammatory factors or hypoxia alter secretomes so that it modified more effective treatment. Paracrine secreted by improve survival other cell populations several mechanisms, immunomodulatory (mostly anti-inflammatory) anti-apoptotic partly based on Hsp27 upregulation. Reproductive medicine is one fields this cell-free approach has been extensively researched. This review presents possible applications challenges using treatment infertility. secretions shown beneficial effects models female male infertility resulting toxic damage, endocrine disorders, trauma, infectious agents, autoimmune origin.

Language: Английский

Revolutionizing Implantation Studies: Uterine-Specific Models and Advanced Technologies

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 450 - 450

Published: March 20, 2025

Implantation is a complex and tightly regulated process essential for the establishment of pregnancy. It involves dynamic interactions between receptive uterus competent embryo, orchestrated by ovarian hormones such as estrogen progesterone. These regulate proliferation, differentiation, gene expression within three primary uterine tissue types: myometrium, stroma, epithelium. Advances in genetic manipulation, particularly Cre/loxP system, have enabled vivo investigation role genes compartmental cell type-specific manner, providing valuable insights into biology during pregnancy disease. The development endometrial organoids has further revolutionized implantation research. They mimic native structure function, offering powerful platform studying hormonal responses, implantation, maternal-fetal interactions. Combined with omics technologies, these models uncovered molecular mechanisms signaling pathways that implantation. This review provides comprehensive overview uterine-specific tools, organoids, omics. We explore how advancements enhance our understanding biology, receptivity, decidualization reproductive

Language: Английский