Difference in vaginal microbiota composition between infertile and fertile patients: A prospective study DOI Creative Commons

Pei-Chen Chen,

Shih‐Fen Chen,

Hsiu‐Jung Tung

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Abstract Background Dysbiosis of the vaginal microbiota, characterized by loss Lactobacillus spp. (classified into five community state types: CSTs, I-V) dominance, is associated with female infertility. In this research, while role CST I, II, III, and V has gained considerable attention, studies focusing on IV are limited. This prospective study aimed to analyze differences in composition a particular focus IVA IVB, between fertile infertile females. Results The microbiota 15 females seven were analyzed using 16S rRNA gene sequence method. differed from that Particularly, percentages several bacterial species, including Oscillospira spp., significantly lower than 31.54% 42.32%, respectively. Analyzing abundance two groups revealed percentage was higher (29.75%) (21.61%). Further analysis demonstrated more prevalent females, constituting 7% total count, compared only 0.94% growth rate Prevotella (95.18%) (69.77%). However, no IVB observed groups. Conclusions potentially creating an unfavorable environment for sperm resulting unexplained Oscillospira abundant indicating healthy microbiota.

Language: Английский

Chronic endometritis and the endometrial microbiota: implications for reproductive success in patients with recurrent implantation failure DOI Creative Commons

Hong Zhang,

Heng Zou,

Chanyu Zhang

et al.

Annals of Clinical Microbiology and Antimicrobials, Journal Year: 2024, Volume and Issue: 23(1)

Published: May 30, 2024

Abstract Background Chronic endometritis (CE) is associated with poor reproductive outcomes, yet the role of endometrial microbiota in patients recurrent implantation failure (RIF) and CE remains unclear. This study aims to characterize RIF assess its implications for outcomes. Methods In this prospective study, we enrolled both without CE. Endometrial cervical samples were collected 16 S rRNA gene sequencing. Microbiota composition was compared between groups using diversity indices, phylum, genus-level analysis. Canonical correlation analysis (CCA) Spearman’s coefficients used relationships CE, microbiota. Predictive functional profiling performed evaluate metabolic pathways Results exhibited greater evenness non-CE patients. Principal coordinates (PCoA) revealed distinct clustering groups. Linear discriminant (LDA) identified Proteobacteria , Aminicenantales Chloroflexaceae as characteristic while Lactobacillus Acinetobacter Herbaspirillum Ralstonia Shewanela Micrococcaceae non-CE. CCA demonstrated associations adverse specific bacterial taxa. Microbial significantly differed groups, enrichment related cofactors, vitamins, secondary metabolites, immune system Conclusion exhibit compositions The increased microbial altered suggest a potential although further studies are necessary elucidate causal relationship alterations fertility. Modulating microbiome may represent novel therapeutic strategy improve IVF outcomes

Language: Английский

Citations

9
Endometrial factors and pregnancy loss frequency in recurrent pregnancy loss patients: comparing RT-PCR microbiology, microbial cultures, and immunohistochemistry of endometrium biopsy DOI Creative Commons
Katarzyna Klimaszyk, Przemysław Wirstlein,

Katarzyna Bednarek-Rajewska

et al.

Journal of Applied Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Abstract The objective of this study is to investigate the presence bacteria in endometrial samples from patients with recurrent pregnancy loss (RPL) and explore potential correlations between bacterial presence, chronic endometritis, previous history. Endometrial 90 RPL were analysed using RT-PCR detect 10 specific species. A subgroup 65 underwent additional microbial culture immunohistochemistry for plasma cell identification. Correlations number losses evaluated. We detected at least one out chosen DNA by 24.4% (22/90) samples. Patients PCR-identified had a significantly higher (median 3 vs 2, p = 0.01). No correlation was observed endometritis diagnosis. significant found detection PCR ( 0.03), though methods fewer positive cases. In patients, detecting selected species correlates losses. However, does not correlate diagnosis based on CD138 immunohistochemistry-identified count. These findings suggest role that may be independent classical inflammatory response associated endometritis.

Language: Английский

Citations

0
A Standard Pipeline for Analyzing the Endometrial Microbiome DOI

Mychelle de Medeiros Garcia Torres,

Daniel Carlos Ferreira Lanza

Reproductive Sciences, Journal Year: 2024, Volume and Issue: 31(8), P. 2163 - 2173

Published: May 8, 2024

Language: Английский

Citations

3
Uterine Endometrium Microbiome in Women with Repeated Implantation Failure Complicated by Endometriosis DOI Open Access
Yosuke Ono, Yuta Kobayashi, Shigeki Shimada

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(16), P. 4605 - 4605

Published: Aug. 7, 2024

Objectives: This prospective study evaluated whether endometriosis is associated with chronic endometritis (CE) and affects the uterine endometrium microbiome (UEM) in women repeated implantation failure (RIF). Methods: Forty-three RIF were divided into 12 (EM) 31 without (non-EM). The UEM was examined by 16S ribosomal RNA (rRNA) sequencing, CE determined CD 138 staining (plasma cells > 5.15/10 mm2) simultaneously. Results: EM group had a higher bacterial number (EM vs. non-EM; median [range], 6.5 3 [3–11, 1–16], p = 0.009), while frequency of Lactobacillus species did not change. rates presence Dialister (41.7% [5/12] 3.3% [1/31], 0.004) Streptococcus (58.3% [7/12] 16.1% [5/31], 0.017) group. prevalence differ between two groups. Multivariable logistic regression analysis revealed that (odds ratio, 10.97, 95% confidence interval, 1.17–249.37, 0.036) endometriosis. In group, five (10 5 [6–11, 3–7], 0.021) Shannon diversity index (0.50 0.20 [0.19–1.39, 0.03–0.46], 0.026) than seven species. Conclusions: species, increased may be related to pathogenesis complicated

Language: Английский

Citations

3
Bridging the Diagnostic Gap between Histopathologic and Hysteroscopic Chronic Endometritis with Deep Learning Models DOI Creative Commons
Kotaro Kitaya,

Tadahiro Yasuo,

Takeshi Yamaguchi

et al.

Medicina, Journal Year: 2024, Volume and Issue: 60(6), P. 972 - 972

Published: June 12, 2024

Chronic endometritis (CE) is an inflammatory pathologic condition of the uterine mucosa characterized by unusual infiltration CD138(+) endometrial stromal plasmacytes (ESPCs). CE often identified in infertile women with unexplained etiology, tubal factors, endometriosis, repeated implantation failure, and recurrent pregnancy loss. Diagnosis has traditionally relied on biopsy histopathologic/immunohistochemistrical detection ESPCs. Endometrial biopsy, however, a somewhat painful procedure for subjects does not allow us to grasp whole picture this mucosal tissue. Meanwhile, fluid hysteroscopy been recently adopted as less-invasive diagnostic modality CE. We launched ARCHIPELAGO (ARChival Hysteroscopic Image-based Prediction histopathologic chronic Endometritis using deep LeArninG mOdel) study construct hysteroscopic finding-based prediction tools The development these learning-based novel models computer-aided detection/diagnosis systems potentially benefits suffering from elusive disease.

Language: Английский

Citations

2
Microbiota and Recurrent Pregnancy Loss (RPL); More than a Simple Connection DOI Open Access
Jenny Garmendia, Claudia Valentina De Sanctis, Marián Hajdúch

et al.

Published: July 9, 2024

Recurrent Pregnancy Loss (RPL) affects 1-2% of women, and its triggering factors are unclear. Several studies have shown the vaginal, endometrial, gut microbiota may play a role in RPL. A decrease quantity Lactobacillus crispatus local has been associated with an increase (vaginal endometrial) inflammatory response immune cell activation that leads to pregnancy loss. The be triggered by gram-negative bacteria, lipopolysaccharides (LPS), viral infections, mycosis, or atypia (tumor growth). Bacterial structures metabolites produced could involved modulation responsible for molecular mimicry. Gut metabolic products amount circulating pro-inflammatory lymphocytes, which, turn, will migrate into vaginal endometrial tissues. Local proinflammatory Th1 Th17 subpopulations along Treg tolerogenic NK cells accountable modulate increasing success. Analyzing necessary characterize some RPL patients. Although oral supplementation probiotics not modify microbiota, it benefit transplantation vagina enhance required achieve normal pregnancy. effect hormone stimulation progesterone maintain early on adequately studied, more research is needed this area. Well-designed clinical trials ascertain

Language: Английский

Citations

2
Chronic endometritis and recurrent reproductive failure: a systematic review and meta-analysis DOI Creative Commons
Carlo Ticconi, Annalisa Inversetti,

Serena Marraffa

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 16, 2024

Background The endometrium holds a crucial role in reproduction by supporting blastocyst adhesion, cytotrophoblast invasion and fetal development. Among the various uterine disorders, endometritis, particularly chronic endometritis (CE), has gained attention due to its association with adverse reproductive outcomes (recurrent pregnancy loss (RPL), recurrent implantation failure (RIF), infertility). between CE stresses necessity for comprehensive diagnostic therapeutic strategies optimize fertility support individuals their journey towards parenthood. Aim To explore relationship disorders. Methods Following PRISMA guidelines, systematic review meta-analysis using published data from 1990 2024 were carried out. Results A population of 1,038 women was included. Regarding CE-infertility association, positive correlation found, 19.46% rate infertile compared 7.7% controls (OR: 2.96, 95% CI 1.53-5.72, p 0.001). No significant observed RIF 1.10, 0.26-4.61, 0.90), rates both groups relatively comparable, 6.35% 5.8% controls. On opposite, strong RPL reporting 37.6% cases 16.4% 3.59, 2.46-5.24, < 0.00001). Conclusions appears be associated infertility RPL, while no noted RIF. Systematic registration https://www.crd.york.ac.uk/prospero/#recordDetails PROSPERO, identifier CRD42024541879.

Language: Английский

Citations

2
Management strategies following implantation failure of euploid embryos DOI Creative Commons
Keiji Kuroda

Reproductive Medicine and Biology, Journal Year: 2024, Volume and Issue: 23(1)

Published: Jan. 1, 2024

Euploid blastocyst implantation failure may result from embryonic factors undetectable by preimplantation genetic testing for aneuploidy (PGT-A); however, various nonembryonic can also intricately interfere with implantation. This review seeks to clarify evidence-based and treatments after euploid embryo transfer.

Language: Английский

Citations

1
Microbiota and Recurrent Pregnancy Loss (RPL); More than a Simple Connection DOI Creative Commons
Jenny Garmendia, Claudia Valentina De Sanctis, Marián Hajdúch

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(8), P. 1641 - 1641

Published: Aug. 10, 2024

Recurrent Pregnancy Loss (RPL) affects 1–2% of women, and its triggering factors are unclear. Several studies have shown that the vaginal, endometrial, gut microbiota may play a role in RPL. A decrease quantity Lactobacillus crispatus local has been associated with an increase (vaginal endometrial) inflammatory response immune cell activation leads to pregnancy loss. The be triggered by gram-negative bacteria, lipopolysaccharides (LPS), viral infections, mycosis, or atypia (tumor growth). Bacterial structures metabolites produced could involved modulation responsible for molecular mimicry. Gut metabolic products amount circulating pro-inflammatory lymphocytes, which, turn, will migrate into vaginal endometrial tissues. Local Th1 Th17 subpopulations Treg tolerogenic NK cells accountable modulate response, increasing success. Analyzing necessary characterize some RPL patients. Although oral supplementation probiotics not modify microbiota, it benefit transplantation vagina enhance required achieve normal pregnancy. effect hormone stimulation progesterone maintain early on adequately studied, more research is needed this area. Well-designed clinical trials ascertain

Language: Английский

Citations

1
Progress of Platelet-Rich Plasma in the Repair of Endometrial Injury DOI

若楠 汤

Advances in Clinical Medicine, Journal Year: 2024, Volume and Issue: 14(01), P. 285 - 291

Published: Jan. 1, 2024

Language: Английский

Citations

0