Photobiomodulation improves functional recovery after mild traumatic brain injury DOI Creative Commons
Andrew R. Stevens, Mohammed A. Hadis, Abhinav Thareja

et al.

Bioengineering & Translational Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

Mild traumatic brain injury (mTBI) is a common consequence of head but there are no recognized interventions to promote recovery the brain. We previously showed that photobiomodulation (PBM) significantly reduced number apoptotic cells in adult rat hippocampal organotypic slice cultures. In this study, we first optimized PBM delivery parameters for use mTBI, conducting cadaveric studies calibrate 660 and 810 nm lasers transcutaneous cortical surface. then used an vivo weight drop mTBI model rats delivered daily doses 660, nm, or combined 660/810 PBM. Functional was assessed using novel object recognition (NOR) beam balance tests, whilst histology immunohistochemistry were assess neuropathology. found at 810, 810/660 all improved both NOR performance, with having greatest effects. Histology demonstrated overt structural damage after however, sections activation CD11b+ microglia glial fibrillary acidic protein (GFAP)+ astrocytes 3 days post-injury. Significantly localization apoptosis marker, cleaved caspase-3, modest reductions extracellular matrix deposition treatment, limited choroid plexus periventricular areas also observed. Our results demonstrate optimally functional outcomes markers associated astrocyte/microglial activation, thus may be useful as potential regenerative therapy.

Language: Английский

Applications of Stem Cell-Derived Extracellular Vesicles in Nerve Regeneration DOI Open Access
Burçak Yavuz, Esra Cansever Mutlu, Zubair Ahmed

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5863 - 5863

Published: May 28, 2024

Extracellular vesicles (EVs), including exosomes, microvesicles, and other lipid derived from cells, play a pivotal role in intercellular communication by transferring information between cells. EVs secreted progenitor stem cells have been associated with the therapeutic effects observed cell-based therapies, they also contribute to tissue regeneration following injury, such as orthopaedic surgery cases. This review explores involvement of nerve regeneration, their potential drug carriers, significance cell research cell-free therapies. It underscores importance bioengineers comprehending manipulating EV activity optimize efficacy engineering regenerative

Language: Английский

Citations

8
GFAPβ and GFAPδ Isoforms Expression in Mesenchymal Stem Cells, MSCs Differentiated Towards Schwann-like, and Olfactory Ensheathing Cells DOI Creative Commons
N. González, Gabriela Stefania Reyes Gutierrez, Tania Campos-Ordoñez

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(1), P. 35 - 35

Published: Jan. 9, 2025

Olfactory ensheathing cells (OECs) and mesenchymal stem (MSCs) differentiated towards Schwann-like have plasticity properties. These express the Glial fibrillary acidic protein (GFAP), a type of cytoskeletal that significantly regulates many cellular functions, including those promote needed for regeneration. However, expression GFAP isoforms (α, β, δ) in these has not been characterized. We evaluated by Polymerase Chain Reaction (PCR) assay three conditions: (1) OECs, (2) exposed to OECs-conditioned medium (dBM-MSCs), (3) MSC cell culture from rat bone marrow undifferentiated (uBM-MSCs). First, characterization phenotyping was verified morphology immunocytochemistry, using p75, CD90, antibodies. Then, we found conditions; GFAPα (10.95%AUC) GFAPβ (9.17%AUC) predominantly followed dBM-MSCs (α: 3.99%AUC, β: 5.66%AUC) uBM-MSCs 2.47%AUC, 2.97%AUC). GFAPδ isoform similar groups (OEC: 9.21%AUC, dBM-MSCs: 11.10%AUC, uBM-MSCs: 9.21%AUC). findings suggest different may regulate properties, potentially contributing tissue remodeling processes dBM-MSCs, uBM-MSCs.

Language: Английский

Citations

0
Evaluation of transcriptomic changes after photobiomodulation in spinal cord injury DOI Creative Commons
Andrew R. Stevens, Mohammed A. Hadis,

Hannah Alldrit

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 25, 2025

Abstract Spinal cord injury (SCI) is a significant cause of lifelong disability, with no available disease-modifying treatments to promote neuroprotection and axon regeneration after injury. Photobiomodulation (PBM) promising therapy which has proven effective at restoring lost function SCI in pre-clinical models. However, the precise mechanism action yet be determined. Here, we used an in-vivo model adult rats that received daily PBM (660 nm, 24 mW/cm 2 , 1 min) three days post-injury, injured spinal segment was harvested subjected whole transcriptome sequencing subsequent pathway analysis (generally applicable gene-set enrichment (GAGE)). Pathway demonstrated 1275 differentially expressed genes (DEGs) treatment, 397 were upregulated 878 downregulated. Key pathways significantly enriched, including 8.6-fold “neuron projection morphogenesis” (adjusted p = 8.10 × 10 − 14 ), upregulation Notch3, Slit1/Robo2 Sema3g pathways. Ribosomal oxidative phosphorylation NADH dehydrogenase downregulated, there ATP-dependent activity, cAMP calcium signalling apoptotic as S100 cyclo-oxygenase components. Together, our study supports favourable effects promoting neuroregeneration suppressing apoptosis neurological Further findings from suggest downregulation metabolism-associated by acute post-injury mitochondrial dysfunction may averted therapy.

Language: Английский

Citations

0
Inhibiting Cell Inspection Points Intervention Via Injectable Short Fibers for Reversing Neural Cell Senescence DOI
Qianyi Li, Liang Chen, Jie Yu

et al.

Advanced Fiber Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Language: Английский

Citations

0
Methylphenidate for the cognitive and neurobehavioural sequelae of traumatic brain injury in adults: a systematic review and meta-analysis DOI Creative Commons
Jonathan Rees, Rachel Saunders, Carl R. Krynicki

et al.

Frontiers in Neurology, Journal Year: 2025, Volume and Issue: 16

Published: March 5, 2025

Traumatic brain injury (TBI) is a leading cause of death and disability globally associated with long-term cognitive neurobehavioural deficits. Methylphenidate has been proposed to address these lasting symptoms, however comprehensive evidence lacking. This systematic review aimed assess the effects methylphenidate on multiple domains in adults TBI. The search conducted across five databases yielded 1,019 results, which 25 were relevant this review. Meta-analyses where homogenous data was available. Significant results favouring recorded by meta-analyses for one cognition outcome measures (Trail Making Test A) (p = 0.005, CI [-5.19, -0.91]), as well depression domain < 0.00001, [-0.78, -0.39]) fatigue [-0.98, -0.67]). Insufficient available aggression, apathy, agitation, memory, motor function, post-concussion syndrome sleep inclusion meta-analysis. Qualitative found limited mixed efficacy methylphenidate, though significant benefits have demonstrated various small, randomised studies. Eleven studies judged containing some high risk bias. However, identified supportive beneficial improve TBI, possible other symptoms. Heterogeneity bias variable studies, somewhat limiting credibility results. may enhance ongoing care TBI patients, addressing symptoms simultaneously. Further large-scale high-quality clinical trials evaluating range should be more conclusively elucidate potential

Language: Английский

Citations

0
Prolonged loss of nuclear HMGB1 in neurons following modeled TBI and implications for long-term genetic health DOI Creative Commons

Laura R Siracusa,

E. Park,

Elaine Liu

et al.

Brain Research, Journal Year: 2025, Volume and Issue: unknown, P. 149559 - 149559

Published: March 1, 2025

Under normal physiological conditions high mobility group box protein 1 (HMGB1) stabilizes chromatin, controls transcription, and contributes to DNA repair. Cellular stress or injury results in HMGB1 release from the nucleus acting as a proinflammatory cytokine. The objective of this study was characterize temporal progression nuclear loss up one week following modeled TBI 250 g male rats correlate these changes with response damage proteins. present cytoplasm absent neurons within 6 h injury. Quantitative immunohistochemistry Western blot analysis showed significant decrease expression at 24 post-injury compared controls. Approximately 20 % were lacking 7 days post-injury. Cells which negative for labelled positive HIF1α, PARP, γH2AX, indicators oxidative damage. Nuclear HIF1α detected after γH2AX observed post-injury, suggesting activation mechanisms repair pathways. translocation conjunction markers suggest relationship between injury-induced subsequent These highlight potential mechanism long-term implications relation genetic health surviving neurons.

Language: Английский

Citations

0
Photobiomodulation improves functional recovery after mild traumatic brain injury DOI Creative Commons
Andrew R. Stevens, Mohammed A. Hadis, Abhinav Thareja

et al.

Bioengineering & Translational Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

Mild traumatic brain injury (mTBI) is a common consequence of head but there are no recognized interventions to promote recovery the brain. We previously showed that photobiomodulation (PBM) significantly reduced number apoptotic cells in adult rat hippocampal organotypic slice cultures. In this study, we first optimized PBM delivery parameters for use mTBI, conducting cadaveric studies calibrate 660 and 810 nm lasers transcutaneous cortical surface. then used an vivo weight drop mTBI model rats delivered daily doses 660, nm, or combined 660/810 PBM. Functional was assessed using novel object recognition (NOR) beam balance tests, whilst histology immunohistochemistry were assess neuropathology. found at 810, 810/660 all improved both NOR performance, with having greatest effects. Histology demonstrated overt structural damage after however, sections activation CD11b+ microglia glial fibrillary acidic protein (GFAP)+ astrocytes 3 days post-injury. Significantly localization apoptosis marker, cleaved caspase-3, modest reductions extracellular matrix deposition treatment, limited choroid plexus periventricular areas also observed. Our results demonstrate optimally functional outcomes markers associated astrocyte/microglial activation, thus may be useful as potential regenerative therapy.

Language: Английский

Citations

0