Parecoxib Enhances Resveratrol against Human Colorectal Cancer Cells through Akt and TXNDC5 Inhibition and MAPK Regulation DOI Open Access

Wan‐Ling Chang,

Kai‐Chien Yang,

Jyun-Yu Peng

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(17), P. 3020 - 3020

Published: Sept. 6, 2024

In this study, we discovered the mechanisms underlying parecoxib and resveratrol combination’s anti-cancer characteristics against human colorectal cancer DLD-1 cells. We studied its anti-proliferation apoptosis-provoking effect by utilizing cell viability 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, fluorescence microscope, gene overexpression, Western blot, flow cytometry analyses. Parecoxib enhanced ability of to inhibit increase apoptosis. in combination with strongly apoptosis inhibiting expression thioredoxin domain containing 5 (TXNDC5) Akt phosphorylation. resveratrol-provoked c-Jun N-terminal kinase (JNK) p38 Overexpression TXNDC5 repression JNK pathways significantly reversed inhibition stimulation parecoxib/resveratrol combination. This study presents evidence that enhances power cells via signaling enhancement JNK/p38 MAPK pathways. may be provided as an efficient drug sensitize resveratrol.

Language: Английский

Parecoxib Enhances Resveratrol against Human Colorectal Cancer Cells through Akt and TXNDC5 Inhibition and MAPK Regulation DOI Open Access

Wan‐Ling Chang,

Kai‐Chien Yang,

Jyun-Yu Peng

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(17), P. 3020 - 3020

Published: Sept. 6, 2024

In this study, we discovered the mechanisms underlying parecoxib and resveratrol combination’s anti-cancer characteristics against human colorectal cancer DLD-1 cells. We studied its anti-proliferation apoptosis-provoking effect by utilizing cell viability 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, fluorescence microscope, gene overexpression, Western blot, flow cytometry analyses. Parecoxib enhanced ability of to inhibit increase apoptosis. in combination with strongly apoptosis inhibiting expression thioredoxin domain containing 5 (TXNDC5) Akt phosphorylation. resveratrol-provoked c-Jun N-terminal kinase (JNK) p38 Overexpression TXNDC5 repression JNK pathways significantly reversed inhibition stimulation parecoxib/resveratrol combination. This study presents evidence that enhances power cells via signaling enhancement JNK/p38 MAPK pathways. may be provided as an efficient drug sensitize resveratrol.

Language: Английский

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